Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,566,154 A→T 6.9% P266P (CCT→CCA)  pctB ← methyl‑accepting chemotaxis protein PctB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,566,1540AT 6.9% 175.6 / 3.0 58P266P (CCT→CCA) pctBmethyl‑accepting chemotaxis protein PctB
Reads supporting (aligned to +/- strand):  ref base A (31/23);  new base T (2/2);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

ATCAGCACCGGAATCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGTCTTTATTGGG  >  NC_002947/2566008‑2566291
                                                                                                                                                  |                                                                                                                                         
atcaGCACCGGAATCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAagg                                                                                                                                         <  1:157273/149‑1 (MQ=255)
   aGCACCGGAATCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAaggcag                                                                                                                                      <  5:29953/149‑1 (MQ=255)
   aGCACCGGAATCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAaggca                                                                                                                                       <  6:220466/148‑1 (MQ=255)
   aGCACCGGAATCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAaggca                                                                                                                                       <  4:114043/148‑1 (MQ=255)
         ggAATCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAggccggc                                                                                                                                >  6:31512/1‑149 (MQ=255)
             tCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGAAGGCCGGCCAcc                                                                                                                            >  7:33246/1‑149 (MQ=255)
                gcaggccgagcaggccgGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCggg                                                                                                                         >  4:70155/1‑149 (MQ=255)
                        agcaggccgGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAAc                                                                                                                   >  5:265903/1‑147 (MQ=255)
                                 gCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTTGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCACGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCg                                                                                                        <  2:262997/149‑1 (MQ=255)
                                 gCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCg                                                                                                        >  1:215409/1‑149 (MQ=255)
                                  cGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCgg                                                                                                       >  3:173443/1‑149 (MQ=255)
                                            cACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGt                                                                                             >  3:76289/1‑149 (MQ=255)
                                               ggCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTc                                                                                            >  2:260678/1‑147 (MQ=255)
                                               ggCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTc                                                                                            >  7:78433/1‑147 (MQ=255)
                                                  aaTCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAgg                                                                                        <  8:33246/148‑1 (MQ=255)
                                                    tCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACgaga                                                                                                               <  2:57361/123‑1 (MQ=255)
                                                    tCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACgaga                                                                                                               >  1:57361/1‑123 (MQ=255)
                                                       gCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCTCTGCGCCGATGGCAGGCCGGCCACCGGGGCGAACGAgatg                                                                                                             <  5:7959/122‑1 (MQ=255)
                                                       gCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCTCTGCGCCGATGGCAGGCCGGCCACCGGGGCGAACGAgatg                                                                                                             >  6:7959/1‑122 (MQ=255)
                                                       gCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGATGGCAGGCCGGCCACCGGGGCGAACGAgatg                                                                                                             <  5:3640/122‑1 (MQ=255)
                                                       gCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGATGGCAGGCCGGCCACCGGGGCGAACGAgatg                                                                                                             >  6:3640/1‑122 (MQ=255)
                                                       gCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACa                                                                                  >  8:176470/1‑149 (MQ=255)
                                                        cATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTg                                                                                                  <  7:291806/132‑1 (MQ=255)
                                                        cATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTg                                                                                                  >  8:291806/1‑132 (MQ=255)
                                                         aTGGCAATGATCGCCGAGTTGCGGAACTGGATGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGTTGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCTAACGAGATGATGCGGTCCTGGCCGTCGAgg                                                                                        <  7:209131/141‑1 (MQ=255)
                                                         aTGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCCGTCCTGGCCGTCGATGgagatcg                                                                                 >  8:209131/1‑145 (MQ=255)
                                                            gCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCt                                                                              <  4:173443/148‑1 (MQ=255)
                                                                    cGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCcgg                                                                     >  4:170332/1‑149 (MQ=255)
                                                                      ccGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCcggcg                                                                   <  8:78433/149‑1 (MQ=255)
                                                                       cGAGGTGCGGATCTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCcggcgg                                                                  <  1:260678/149‑1 (MQ=255)
                                                                                gAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCg                                                           >  7:82523/1‑147 (MQ=255)
                                                                                   cTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGt                                                      <  8:51137/149‑1 (MQ=255)
                                                                                   cTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGt                                                      >  7:51137/1‑149 (MQ=255)
                                                                                     ggCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTtg                                                    >  5:203578/1‑149 (MQ=255)
                                                                                      gCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGtt                                                     <  7:116620/147‑1 (MQ=255)
                                                                                       cTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTtgct                                                  <  5:31512/149‑1 (MQ=255)
                                                                                           gcgcGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCt                                              >  7:3471/1‑149 (MQ=255)
                                                                                           gcgcGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCt                                              <  8:82523/149‑1 (MQ=255)
                                                                                             gcgGCATAGGCCTTGTCCCTGTCGATAGACAGACCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTgg                                            >  7:37676/1‑149 (MQ=255)
                                                                                              cgGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCTGCGGCCACCCGCAGGTTGCTGCCTggg                                           <  6:265903/149‑1 (MQ=255)
                                                                                               ggCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAgg                                                                                        >  1:163681/1‑103 (MQ=255)
                                                                                               ggCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAgg                                                                                        <  2:163681/103‑1 (MQ=255)
                                                                                                      gCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGAt                                      <  6:42569/146‑1 (MQ=255)
                                                                                                      gCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGAt                                      >  5:42569/1‑146 (MQ=255)
                                                                                                      gCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTc                                   >  3:297543/1‑149 (MQ=255)
                                                                                                      gCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTc                                   >  5:137081/1‑149 (MQ=255)
                                                                                                            tCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGa                              <  3:170332/148‑1 (MQ=255)
                                                                                                              ccTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGt                           >  6:35529/1‑149 (MQ=255)
                                                                                                              ccTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATCCCGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGATGCAGCCTGGGTAGATGTCCTTGAGGt                           >  4:115180/1‑149 (MQ=255)
                                                                                                               cTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGtt                          <  7:176470/149‑1 (MQ=255)
                                                                                                                  tcgatcgaCAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGttct                        <  6:203578/148‑1 (MQ=255)
                                                                                                                    gatcgaCAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGttcttc                      <  3:70155/148‑1 (MQ=255)
                                                                                                                     atcgaCAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTgg                                            >  5:255151/1‑125 (MQ=255)
                                                                                                                     atcgaCAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTgg                                            <  6:255151/125‑1 (MQ=255)
                                                                                                                         aCAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCAcc                >  2:102904/1‑149 (MQ=255)
                                                                                                                             cccGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGt            >  5:190209/1‑149 (MQ=255)
                                                                                                                             cccGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGt            <  8:3471/149‑1 (MQ=255)
                                                                                                                                    tACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGTCTTTAtt     <  2:215409/149‑1 (MQ=255)
                                                                                                                                     aCCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGTCTTTATTg    >  8:229570/1‑149 (MQ=255)
                                                                                                                                       cACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGTCTTTATTggg  >  5:307494/1‑149 (MQ=255)
                                                                                                                                         cTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTTACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGTCt          <  3:219753/139‑1 (MQ=255)
                                                                                                                                         cTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGTCt          >  4:219753/1‑139 (MQ=255)
                                                                                                                                                  |                                                                                                                                         
ATCAGCACCGGAATCAGCAGGCCGAGCAGGCCGGCGATGGCGGCCACGGCAATCAGCATGGCAATGATCGCCGAGGTGCGGAACTGGCTGAGCGCGGCATAGGCCTTGTCCCTGTCGATCGACAGCCCGATGTACCACTGCGCCGAAGGCAGGCCGGCCACCGGGGCGAACGAGATGATGCGGTCCTGGCCGTCGAGGGTGACATCCTGCATGCCGGCGGCCACCCGCAGGTTGCTGCCTGGGTAGATGTCCTTGAGGTTCTTCATCACCTGGTCTTTATTGGG  >  NC_002947/2566008‑2566291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑