Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,636,659 A→C 7.3% intergenic (‑86/‑86) PP_2305 ← / → PP_2306 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,636,6590AC 7.3% 164.5 / 5.0 55intergenic (‑86/‑86)PP_2305/PP_2306hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/20);  new base C (2/2);  total (33/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGCGCATACCCTCGGGCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGCGCGGGCAAGAC  >  NC_002947/2636512‑2636806
                                                                                                                                                   |                                                                                                                                                   
tgCGCATACCCTCGGGCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAAc                                                                                                                                                    <  7:139394/149‑1 (MQ=255)
   gcATACCCTCGGGCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTTCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGTTCGTTCTGAGCTACTGTAATAAAACTgg                                                                                                                                                 <  2:143155/149‑1 (MQ=255)
     aTACCCTCGGGCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGc                                                                                                                                                >  3:205749/1‑148 (MQ=255)
         ccTCGGGCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGc                                                                                                                                           >  1:263280/1‑149 (MQ=255)
           tCGGGCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCCCCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCGACGGTAATCAAACTGGCCAGGCa                                                                                                                                          >  5:186331/1‑148 (MQ=255)
              ggCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCACGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCATGTGATTAAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGTCCAGGAAAAGc                                                                                                                                      >  2:282756/1‑149 (MQ=255)
                 tCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCgg                                                                                                                                   >  3:211721/1‑149 (MQ=255)
                        gATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCa                                                                                                                            >  8:185037/1‑149 (MQ=255)
                         aTCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCAt                                                                                                                           <  2:263280/149‑1 (MQ=255)
                            ccAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATgg                                                                                                                         >  7:152728/1‑148 (MQ=255)
                                  ccAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCGTCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACttt                                                                                                                   <  6:303417/148‑1 (MQ=255)
                                        ccACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTg                                                                                                            >  5:167890/1‑149 (MQ=255)
                                          acacGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTc                                                                                                          <  5:202718/149‑1 (MQ=255)
                                                  aCTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGt                                                                                                   <  5:130536/148‑1 (MQ=255)
                                                    tCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCg                                                                                                 >  2:79830/1‑148 (MQ=255)
                                                      aTGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAg                                                                                                      >  5:19862/1‑141 (MQ=255)
                                                      aTGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAg                                                                                                      <  6:19862/141‑1 (MQ=255)
                                                         aaCATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGc                                                                                           <  8:152728/149‑1 (MQ=255)
                                                          aCATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTgtc                                                                                                                                                <  1:40493/95‑3 (MQ=255)
                                                          aCATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGc                                                                                                                                                >  2:40493/1‑95 (MQ=255)
                                                              tCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCg                                                                                      >  8:262678/1‑149 (MQ=255)
                                                              tCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCg                                                                                      >  3:181523/1‑149 (MQ=255)
                                                                cccGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGtt                                                                                    >  2:203658/1‑149 (MQ=255)
                                                                 ccGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCCCCGttt                                                                                   >  4:36273/1‑149 (MQ=255)
                                                                    gCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTc                                                                                <  4:211721/149‑1 (MQ=255)
                                                                        gTGCCATCATGGTCCTACAATGCACCCATCGCTGCCAGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATa                                                                            <  7:169361/149‑1 (MQ=255)
                                                                          gCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGGTTTTCCATa                                                                            >  4:275664/1‑147 (MQ=255)
                                                                           ccATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATagag                                                                         <  7:185037/149‑1 (MQ=255)
                                                                                      cTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACtgat                                                              >  3:24207/1‑149 (MQ=255)
                                                                                      cTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACtga                                                               <  6:167890/148‑1 (MQ=255)
                                                                                      cTACAATGCACCCATCGCTGCCTGTGCTTCAATGTACATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACtgat                                                              <  1:282756/149‑1 (MQ=255)
                                                                                        aCAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACtcatg                                                             >  7:278062/1‑148 (MQ=255)
                                                                                            tGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACTGCGACCGTTTTTCCATAGAGGTATTACTGATGAAAGc                                                        <  1:79830/149‑1 (MQ=255)
                                                                                            tGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGc                                                        >  3:296761/1‑149 (MQ=255)
                                                                                                                cTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGa                                    >  2:28339/1‑149 (MQ=255)
                                                                                                                 ttCAATGTAGATTGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTg                                                     >  3:209820/1‑131 (MQ=255)
                                                                                                                 ttCAATGTAGATTGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTg                                                     <  4:209820/131‑1 (MQ=255)
                                                                                                                 ttCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGc                                              <  5:155687/138‑1 (MQ=255)
                                                                                                                 ttCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGc                                              >  6:155687/1‑138 (MQ=255)
                                                                                                                 ttCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGAt                                   >  7:121229/1‑149 (MQ=255)
                                                                                                                    aaTGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGc                                              <  3:911/135‑1 (MQ=255)
                                                                                                                    aaTGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGc                                              >  4:911/1‑135 (MQ=255)
                                                                                                                        tAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTg                            <  4:296761/149‑1 (MQ=255)
                                                                                                                           aTCGTGCTGAGCTACTGTAATAAACCTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGcc                         >  4:122533/1‑149 (MQ=255)
                                                                                                                           aTCGTGCTGAGCTACTGTAATAAACCTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGcc                         >  4:127240/1‑149 (MQ=255)
                                                                                                                           aTCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGcc                         >  7:74360/1‑149 (MQ=255)
                                                                                                                                    aGCTACTGTAATAAACCTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTgctgg                 <  3:122533/148‑1 (MQ=255)
                                                                                                                                    aGCTACTGTAATAAACCTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTgctgg                 <  3:127240/148‑1 (MQ=255)
                                                                                                                                    aGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACTGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTgctggc                >  4:190900/1‑149 (MQ=255)
                                                                                                                                       tACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTgc             >  2:277419/1‑149 (MQ=255)
                                                                                                                                          tGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGcgcg          >  1:240580/1‑149 (MQ=255)
                                                                                                                                           gTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGCGCgg         >  6:77671/1‑149 (MQ=255)
                                                                                                                                            gactaaCACTGGCCCTGCAAAGCCGGCTGGGCATGGCACTTTCCCGCGGTCGGAGCGGCGAACAGCCACCGGTTTTCCATCGAGGTATTACTGATGAAAGCCAGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGcgcg          <  6:186331/144‑1 (MQ=255)
                                                                                                                                             aataaATCTGGCCAGGCAAAGCCGGCTGGGGATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGCGCggg        >  1:23733/1‑148 (MQ=255)
                                                                                                                                               taaAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGCGCGGGc       >  6:44153/1‑147 (MQ=255)
                                                                                                                                                  aaCTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGCGCGGGCAAGAc  >  5:174065/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCGCATACCCTCGGGCTCCGCCCGATCCCAGTACCAGTGCCACACGTTTACTCATGAACATTCCCCGGCCAGTGCCATCATGGTCCTACAATGCACCCATCGCTGCCTGTGCTTCAATGTAGATCGTGCTGAGCTACTGTAATAAAACTGGCCAGGCAAAGCCGGCTGGGCATGGCACTTTCCAGCTGTCGGAGCGTCGAACAGCCACCGTTTTTCCATAGAGGTATTACTGATGAAAGCCTGGATTTGCCTTCCACTGATTGCCCTGGCCCTTGCTGGCTGCGCGGGCAAGAC  >  NC_002947/2636512‑2636806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑