Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,650,160 A→T 9.6% intergenic (+99/+35) PP_2320 → / ← oprI hypothetical protein/major outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,650,1600AT 9.6% 145.8 / 5.3 52intergenic (+99/+35)PP_2320/oprIhypothetical protein/major outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base T (2/3);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTC  >  NC_002947/2650013‑2650308
                                                                                                                                                   |                                                                                                                                                    
ccccggggCCGCACCGATGGTGACGAGCATTCCGCCCGCACAGCAGGAACCGAATTTCAGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACaa                                                                                                                                                     <  6:159499/145‑1 (MQ=255)
 cAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAg                                                                                                                                                    >  5:81590/1‑149 (MQ=255)
  aGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGt                                                                                                                                                   <  5:257209/149‑1 (MQ=255)
        ccGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCg                                                                                                                                             <  1:108956/149‑1 (MQ=255)
         cGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCgg                                                                                                                                            <  5:269666/149‑1 (MQ=255)
         cGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCgg                                                                                                                                            <  6:135388/149‑1 (MQ=255)
          gCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCGACAAGTGGGTCGGc                                                                                                                                           <  6:92998/149‑1 (MQ=255)
              cGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttct                                                                                                                                       <  5:33319/149‑1 (MQ=255)
                   gTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGcttttttat                                                                                                                                   <  8:285030/148‑4 (MQ=255)
                        gAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCg                                                                                                                             >  5:64147/1‑149 (MQ=255)
                           cATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                                                                                          <  7:276150/149‑1 (MQ=255)
                                 gCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGatta                                                                                                                    >  4:118736/1‑149 (MQ=255)
                                        cTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTg                                                                                                             <  1:224711/149‑1 (MQ=255)
                                            aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggc                                                                                                         >  7:16014/1‑149 (MQ=255)
                                             gTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggct                                                                                                        <  6:81590/149‑1 (MQ=255)
                                                 ggTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTaa                                                                                                                                    >  4:255795/1‑117 (MQ=255)
                                                 ggTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctggct                                                                                                    <  3:206406/149‑1 (MQ=255)
                                                  gTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCtt                                                                                                   >  3:216959/1‑149 (MQ=255)
                                                     aTTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGt                                                                                                <  8:16014/149‑1 (MQ=255)
                                                          ggCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAg                                                                                            <  6:64147/148‑1 (MQ=255)
                                                             gTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATa                                                                                        <  3:118736/149‑1 (MQ=255)
                                                                       cTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcgc                                                                              >  5:63314/1‑149 (MQ=255)
                                                                         gCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcgcgc                                                                            >  6:115321/1‑149 (MQ=255)
                                                                            cTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCg                                                                         >  6:258224/1‑149 (MQ=255)
                                                                                  gCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGc                                                                    <  4:247442/148‑1 (MQ=255)
                                                                                           ttCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAg                                                            <  5:115321/147‑1 (MQ=255)
                                                                                             cGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGt                                                        >  6:22952/1‑149 (MQ=255)
                                                                                              gTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGt                                                        <  7:278562/148‑1 (MQ=255)
                                                                                                  ctgctgACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTctgct                                                   <  5:49560/149‑1 (MQ=255)
                                                                                                   tgctgACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTctgct                                                   >  5:130087/1‑148 (MQ=255)
                                                                                                   tgctgACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTctgct                                                   >  7:31642/1‑148 (MQ=255)
                                                                                                          cgcgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTc                                           >  1:305145/1‑149 (MQ=255)
                                                                                                            cgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTg                                         <  4:216959/149‑1 (MQ=255)
                                                                                                                 gCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCACGGAGCGCACGTTGGCTTCGTCAGCGGTCTGATGCGCCATCTGAGCgg                                    >  6:185964/1‑149 (MQ=255)
                                                                                                                  ccGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGTGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCgg                                    <  5:22952/148‑1 (MQ=255)
                                                                                                                  ccGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCgg                                    <  1:25609/148‑1 (MQ=255)
                                                                                                                   cGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCt                                  >  3:116161/1‑149 (MQ=255)
                                                                                                                   cGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCt                                  >  7:87742/1‑149 (MQ=255)
                                                                                                                   cGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCt                                  >  1:258083/1‑149 (MQ=255)
                                                                                                                          aaaGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTg                           >  6:198584/1‑149 (MQ=255)
                                                                                                                          aaaGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCAGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTg                           >  1:95973/1‑149 (MQ=255)
                                                                                                                           aaGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGc                          <  5:198584/149‑1 (MQ=255)
                                                                                                                              gCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGcgtc                       >  6:39521/1‑149 (MQ=255)
                                                                                                                                aaTAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCt                                          >  6:174297/1‑128 (MQ=255)
                                                                                                                                aaTAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCt                                          <  5:174297/128‑1 (MQ=255)
                                                                                                                                 aTAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCgtcgtc                    <  2:305145/149‑1 (MQ=255)
                                                                                                                                  tAAAAAAGCCGACCCACAAGTGGTACGGATTATTAAAAAACGGTGAGGATTTTTACTTGCGGCTGGCTTTGTCAAGAATACGCAGGGCGCGCAAGTTGGCTTCGACAGCGGACTGCTGAGCGGTCTGAGGGGCTGCCATTGcgtcgac                    >  7:194919/1‑146 (MQ=255)
                                                                                                                                      aaaGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCt               >  5:215071/1‑149 (MQ=255)
                                                                                                                                      aaaGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCt               >  1:180911/1‑149 (MQ=255)
                                                                                                                                         gCCGACCCACTTGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTAc            >  6:177126/1‑149 (MQ=255)
                                                                                                                                         gCCGACCCACTTGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTAc            >  1:53798/1‑149 (MQ=255)
                                                                                                                                         gCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTAc            >  2:289722/1‑149 (MQ=255)
                                                                                                                                           cGACCCACTTGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACgg          <  5:177126/149‑1 (MQ=255)
                                                                                                                                                   aaGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTc  >  6:148539/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTC  >  NC_002947/2650013‑2650308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑