Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,651,276	G→C	9.1%	intergenic (+41/+29)	PP_2323 → / ← aroF‑I	hypothetical protein/tyr‑sensitive phospho‑2‑dehydro‑3‑deoxyheptonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,651,276	0	G	C	9.1%	129.5 / 4.5	44	intergenic (+41/+29)	PP_2323/aroF‑I	hypothetical protein/tyr‑sensitive phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base C (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GCTCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCATACTGC  >  NC_002947/2651134‑2651419                                                                                                                                               |                                                                                                                                                gCTCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAACGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCg                                                                                                                                           >  8:261679/1‑149 (MQ=255)       gAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAACACGCCGGGCAGTGCCCGGCGttt                                                                                                                                     <  2:159819/149‑1 (MQ=255)       gAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGttt                                                                                                                                     <  8:17296/149‑1 (MQ=255)          tACGCCGAGCAGATGGGGTACACGGTGATCCGGTCTTGCTCATATGTGTAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGttttt                                                                                                                                   <  6:195310/148‑1 (MQ=255)               cGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTcc                                                                                                                             >  6:75956/1‑149 (MQ=255)                      aTGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGttt                                                                                                                                     >  5:129574/1‑134 (MQ=255)                      aTGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGttt                                                                                                                                     <  6:129574/134‑1 (MQ=255)                                         cGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCg                                                                                                                           >  1:291393/1‑125 (MQ=255)                                         cGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCg                                                                                                                           <  2:291393/125‑1 (MQ=255)                                           tCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCg                                                                                                 <  6:291128/149‑1 (MQ=255)                                               gCTCATATGTGGAGCGTTACATGGAACGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGgca                                                                                              <  7:261679/148‑1 (MQ=255)                                                   atatGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGcac                                                                                          <  1:291346/148‑1 (MQ=255)                                                      tgtgGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTAACGACAGGCACCAGACACACCAAAAACCGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATc                                                                                       <  5:72295/148‑1 (MQ=255)                                                       gtgGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCt                                                                                     >  1:103070/1‑149 (MQ=255)                                                        tgGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCtt                                                                                    <  6:108233/149‑1 (MQ=255)                                                           aGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAg                                                                                 <  2:103070/149‑1 (MQ=255)                                                                     ggAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGcc                                                                                                               <  7:265976/109‑1 (MQ=255)                                                                     ggAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGcc                                                                                                               >  8:265976/1‑109 (MQ=255)                                                                           ccAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGACGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGAGGCAGCACATCCTTGAGCTTGGCATGCAtgctg                                                                 >  5:312048/1‑149 (MQ=255)                                                                                         cGACCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTtct                                                    <  8:267059/148‑1 (MQ=255)                                                                                          gAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCg                                                  >  1:211117/1‑149 (MQ=255)                                                                                          gAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCg                                                  >  1:8420/1‑149 (MQ=255)                                                                                            gCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGACAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTtctc                                                   <  8:260145/146‑1 (MQ=255)                                                                                            gCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGACAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTtctc                                                   >  7:260145/1‑146 (MQ=255)                                                                                                      aGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGAcc                                        <  2:8420/147‑1 (MQ=255)                                                                                                           acgacAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGAt                                 >  4:117387/1‑149 (MQ=255)                                                                                                                aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCa                              <  1:282781/147‑1 (MQ=255)                                                                                                                aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCa                              >  2:282781/1‑147 (MQ=255)                                                                                                                  gCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGAt                                 <  5:138611/142‑1 (MQ=255)                                                                                                                  gCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGAt                                 >  6:138611/1‑142 (MQ=255)                                                                                                                  gCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCg                          >  3:174849/1‑149 (MQ=255)                                                                                                                           acaacaAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATc                 >  1:51983/1‑149 (MQ=255)                                                                                                                             aacaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGgcatgca                                                                      <  8:265948/94‑1 (MQ=255)                                                                                                                             aacaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGgcatgca                                                                      >  7:265948/1‑94 (MQ=255)                                                                                                                             aacaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGcatgcatg                                                                    >  4:165319/1‑96 (MQ=255)                                                                                                                             aacaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGcatgcatg                                                                    <  3:165319/96‑1 (MQ=255)                                                                                                                             aacaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGCCCAGGCGATGCAGGCGTACGTGATCGa               >  8:285530/1‑149 (MQ=255)                                                                                                                                 aaaaaCGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGAt                                 <  7:195928/127‑1 (MQ=255)                                                                                                                                 aaaaaCGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGAt                                 >  8:195928/1‑127 (MQ=255)                                                                                                                                 aaaaaCGCCGGGCACTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGGGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGa               >  1:250756/1‑145 (MQ=255)                                                                                                                                 aaaaaCGCCGGGCACTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGa               <  2:250756/145‑1 (MQ=255)                                                                                                                                   aaaCGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGcc         <  3:117387/149‑1 (MQ=255)                                                                                                                                    aaCGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCa        >  1:38365/1‑149 (MQ=255)                                                                                                                                           ggCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCATACTGc  >  6:294786/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                GCTCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCATACTGC  >  NC_002947/2651134‑2651419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑