Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,664,508 T→G 6.0% V124G (GTC→GGC)  acnA‑II → aconitate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,664,5080TG 6.0% 194.4 / 4.0 67V124G (GTC→GGC) acnA‑IIaconitate hydratase
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base G (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

GCCCGCGTGGTGTGCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGC  >  NC_002947/2664360‑2664654
                                                                                                                                                    |                                                                                                                                                  
gCCCGCGTGGTGTGCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGt                                                                                                                                                    <  6:134741/149‑1 (MQ=255)
  ccGCGTGGTGTGCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTc                                                                                                                                                   >  2:229000/1‑148 (MQ=255)
   cgcgTGGTGTGCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTc                                                                                                                                                   >  8:190002/1‑147 (MQ=255)
          tgtgCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCggt                                                                                                                                          >  5:51240/1‑149 (MQ=255)
            tgCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCggtgg                                                                                                                                        >  7:269404/1‑149 (MQ=255)
            tgCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCggtg                                                                                                                                         <  5:28303/148‑1 (MQ=255)
            tgCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGACGGCCAGCGAGTggtcga                                                                                                                                         <  5:195663/148‑6 (MQ=255)
              ccATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCGCAGGTCAACCCGGAGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGtt                                                                                                                                      >  3:261580/1‑149 (MQ=255)
              ccATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCggtggt                                                                                                                                       <  3:232557/148‑1 (MQ=255)
              ccATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGtt                                                                                                                                      <  7:277750/149‑1 (MQ=255)
                 tGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTc                                                                                                                                    >  8:276340/1‑148 (MQ=255)
                       ccTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCg                                                                                                                              >  8:54153/1‑148 (MQ=255)
                         ttGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAg                                                                                                                           >  1:165828/1‑149 (MQ=255)
                                  cTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCTACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGaa                                                                                                                  <  7:247596/149‑1 (MQ=255)
                                  cTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGaa                                                                                                                  <  6:69360/149‑1 (MQ=255)
                                             gACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACcgcg                                                                                                        <  6:51240/148‑1 (MQ=255)
                                               ccTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCa                                                                                                     >  8:35543/1‑149 (MQ=255)
                                                   gCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATc                                                                                                   <  8:269404/147‑1 (MQ=255)
                                                        ccTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCg                                                                                                  >  7:177524/1‑143 (MQ=255)
                                                        ccTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATc                                                                                            <  7:54153/149‑1 (MQ=255)
                                                        ccTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATc                                                                                            <  2:113901/149‑1 (MQ=255)
                                                                aCGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAAc                                                                                    >  7:262274/1‑149 (MQ=255)
                                                                  gCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGa                                                                                  <  7:276340/149‑1 (MQ=255)
                                                                   ccATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACg                                                                                   >  5:170345/1‑147 (MQ=255)
                                                                   ccATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACg                                                                                   >  2:169372/1‑147 (MQ=255)
                                                                   ccATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGaa                                                                                 <  7:138366/149‑1 (MQ=255)
                                                                    cATTGCCGACAAAGGCGGCGACCCAGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCATTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGaaaa                                                                                                                <  2:228453/117‑1 (MQ=255)
                                                                    cATTGCCGACAAAGGCGGCGACCCAGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCAATCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGaaaa                                                                                                                >  1:228453/1‑117 (MQ=255)
                                                                     aTTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGa                                                                               <  7:190002/149‑1 (MQ=255)
                                                                         ccGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGaa                                                                                                             >  4:191659/1‑115 (MQ=255)
                                                                         ccGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGaa                                                                                                             <  3:191659/115‑1 (MQ=255)
                                                                         ccGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGACGGCCAGCGAGTggtc                                                                                                                                           >  2:239352/1‑82 (MQ=38)
                                                                         ccGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGACGGCCAGCGAGTggtc                                                                                                                                           <  1:239352/85‑4 (MQ=38)
                                                                           gACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCtt                                                                         >  6:203378/1‑149 (MQ=255)
                                                                                     gcgACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTtcct                                                                >  8:79066/1‑146 (MQ=255)
                                                                                       gACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCaa                                                             >  5:202904/1‑149 (MQ=255)
                                                                                        aCCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAAc                                                            >  6:236145/1‑149 (MQ=255)
                                                                                                 aGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGAcc                                                      >  6:191870/1‑146 (MQ=255)
                                                                                                   gTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCaagaa                                                 <  8:177524/149‑1 (MQ=255)
                                                                                                    tCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCaagaag                                                <  5:203378/149‑1 (MQ=255)
                                                                                                        cccGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGt                                            >  3:244355/1‑149 (MQ=255)
                                                                                                         ccGGTGGTGCCGGTTCAAGTGATCGTCGACCACTGGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCAACAACTGGACCAAGAAGGCGtt                                           <  4:261580/149‑1 (MQ=255)
                                                                                                         ccGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGtt                                           >  1:4065/1‑149 (MQ=255)
                                                                                                           ggtggtGCCGGTGAAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTc                                          >  4:96267/1‑148 (MQ=255)
                                                                                                             tggtgCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAg                                       <  4:244355/149‑1 (MQ=255)
                                                                                                             tggtgCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACCTCATCAACTGGACCAAGAAGGCGTTCAAg                                       <  2:165828/149‑1 (MQ=255)
                                                                                                                  ccGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAAc                                    >  7:167871/1‑147 (MQ=255)
                                                                                                                  ccGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAAc                                    <  8:167871/147‑1 (MQ=255)
                                                                                                                     gTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTc                                                                        >  8:211541/1‑108 (MQ=255)
                                                                                                                     gTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTc                                                                        <  7:211541/108‑1 (MQ=255)
                                                                                                                         aaCTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGt                            >  7:194782/1‑148 (MQ=255)
                                                                                                                         aaCTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTg                           <  1:169372/149‑1 (MQ=255)
                                                                                                                          aCTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGCCCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATAGCCGCAACGAAGCGCGCTTCCACTTCATCAACTGGACGAAGAAGGCGTTCAAGCACGTCGACGt                            >  8:65084/1‑147 (MQ=255)
                                                                                                                          aCTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAAAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGa                                      <  3:277609/137‑1 (MQ=255)
                                                                                                                          aCTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAAAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGa                                      >  4:277609/1‑137 (MQ=255)
                                                                                                                            tGATCGTCGACCACTCGCTGGCCGTCGAGTGCTGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCaagaa                                                 <  1:283839/124‑1 (MQ=255)
                                                                                                                            tGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCaagaa                                                 >  2:283839/1‑124 (MQ=255)
                                                                                                                              aTCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCa                      <  3:96267/149‑1 (MQ=255)
                                                                                                                                  caccgcACTCGACGGCCAGCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCg                  >  5:230655/6‑149 (MQ=255)
                                                                                                                                     aCCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGAACCAGCCggg                >  1:259390/1‑148 (MQ=255)
                                                                                                                                        aCTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAAc            <  6:202904/149‑1 (MQ=255)
                                                                                                                                         cTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACg           <  7:35543/149‑1 (MQ=255)
                                                                                                                                          tCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACgg          >  1:174530/1‑149 (MQ=255)
                                                                                                                                           cGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACGGc         <  8:262274/149‑1 (MQ=255)
                                                                                                                                                gCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACGGcatcat    <  2:174530/149‑1 (MQ=255)
                                                                                                                                                 ccGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATAGCCGCAACGAACACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCATGAACGTCTACCTGATCAAGACGGGCAACGGcatcagg   >  7:247090/1‑147 (MQ=255)
                                                                                                                                                  cGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGc  <  2:4065/149‑1 (MQ=255)
                                                                                                                                                  cGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGc  <  4:236271/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCCGCGTGGTGTGCCATGACATCCTTGGCCAGACTGCGCTGGTCGACCTTGCCGGCCTGCGTGACGCCATTGCCGACAAAGGCGGCGACCCGGCCCAGGTCAACCCGGTGGTGCCGGTGCAACTGATCGTCGACCACTCGCTGGCCGTCGAGTGCGGTGGTTTCGACCCGCAGGCCTTCGAAAAGAACCGCGCCATCGAAGATCGCCGCAACGAAGACCGCTTCCACTTCATCAACTGGACCAAGAAGGCGTTCAAGAACGTCGACGTGATCCAGCCGGGCAACGGCATCATGC  >  NC_002947/2664360‑2664654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑