Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,763,790 C→T 12.7% A41T (GCC→ACC)  PP_2417 ← iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,763,7900CT12.7% 119.4 / 7.7 47A41T (GCC→ACC) PP_2417iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (24/17);  new base T (3/3);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TTGCGCAACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCG  >  NC_002947/2763642‑2763931
                                                                                                                                                    |                                                                                                                                             
ttGCGCAACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGc                                                                                                                                               <  7:147373/149‑1 (MQ=255)
ttGCGCAACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGc                                                                                                                                               <  3:169965/149‑1 (MQ=255)
  gcgcAACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGcc                                                                                                                                              <  4:252953/148‑1 (MQ=255)
   cgcAACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCa                                                                                                                                             <  6:259147/148‑1 (MQ=255)
      aaCATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCAc                                                                                                                                         <  7:26600/149‑1 (MQ=255)
                      tGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAc                                                                                                                             >  1:136816/1‑145 (MQ=255)
                      tGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAc                                                                                                                             <  2:136816/145‑1 (MQ=255)
                       gACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTgg                                                                                                                        >  4:39373/1‑149 (MQ=255)
                            cacaGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGa                                                                                                                    >  1:2890/1‑148 (MQ=255)
                              caGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAg                                                                                                                 >  7:80197/1‑149 (MQ=255)
                                        gCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCg                                                                                                       <  3:39160/149‑1 (MQ=255)
                                             gcgcCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAAt                                                                                                  >  2:16120/1‑149 (MQ=255)
                                                 cacaAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCt                                                                                                                                       >  4:280061/1‑108 (MQ=255)
                                                 cacaAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCt                                                                                                                                       <  3:280061/108‑1 (MQ=255)
                                                  acaAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCgg                                                                                             >  3:5450/1‑149 (MQ=255)
                                                          ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                           >  4:25876/1‑109 (MQ=38)
                                                          ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                           >  4:21954/1‑109 (MQ=38)
                                                          ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                           <  3:21954/111‑3 (MQ=38)
                                                          ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                           <  3:25876/111‑3 (MQ=38)
                                                                  aGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGggc                                                                             >  5:145170/1‑149 (MQ=255)
                                                                           gTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCaa                                                                       >  4:264701/1‑146 (MQ=255)
                                                                               ggCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCg                                                                >  4:155086/1‑149 (MQ=255)
                                                                                gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                               >  7:32094/1‑149 (MQ=255)
                                                                                gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                               >  2:286769/1‑149 (MQ=255)
                                                                                gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                               >  8:172373/1‑149 (MQ=255)
                                                                                   gTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTGCCGGCCAGCAGGGAGGCCAACAGAGCGGc                                                              >  8:293038/1‑147 (MQ=255)
                                                                                    tAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGcc                                                                         <  2:42827/135‑1 (MQ=255)
                                                                                    tAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGATGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGcc                                                                         >  1:42827/1‑135 (MQ=255)
                                                                                      gTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAggg                                                         >  7:118245/1‑149 (MQ=255)
                                                                                      gTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAgg                                                          <  7:172373/148‑1 (MQ=255)
                                                                                            gACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACcg                                                   <  3:92667/149‑1 (MQ=255)
                                                                                              cGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACcgcg                                                 <  1:286769/149‑1 (MQ=255)
                                                                                                     ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCaa                                                                                                   <  1:143849/92‑1 (MQ=255)
                                                                                                     ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCaa                                                                                                   >  2:143849/1‑92 (MQ=255)
                                                                                                     ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAgg                                          <  6:145170/149‑1 (MQ=255)
                                                                                                       cGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGt                                        <  4:5450/149‑1 (MQ=255)
                                                                                                             tCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAgg                                  <  3:155086/149‑1 (MQ=255)
                                                                                                               gggTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAggag                                >  8:9778/1‑147 (MQ=255)
                                                                                                                ggTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCgg                               <  8:118245/149‑1 (MQ=255)
                                                                                                                    cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                           >  3:279114/1‑149 (MQ=255)
                                                                                                                    cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                           >  3:157527/1‑149 (MQ=255)
                                                                                                                    cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                           >  4:128915/1‑149 (MQ=255)
                                                                                                                    cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatc                            <  2:2890/148‑1 (MQ=255)
                                                                                                                     aCCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatcat                          >  7:265653/1‑149 (MQ=255)
                                                                                                                       cGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGAtcatca                        >  3:11592/1‑149 (MQ=255)
                                                                                                                              gcggcTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGgct                 >  6:55416/1‑149 (MQ=255)
                                                                                                                                      ccACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTAc          <  8:80197/148‑1 (MQ=255)
                                                                                                                                       cacaGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCg        >  1:85736/1‑149 (MQ=255)
                                                                                                                                            gtggtTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGc   <  3:39373/149‑1 (MQ=255)
                                                                                                                                             tggtTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCg  <  8:265653/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TTGCGCAACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCG  >  NC_002947/2763642‑2763931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑