Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,763,795 A→T 12.8% V39E (GTG→GAG) ‡ PP_2417 ← iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,763,7950AT12.8% 118.0 / 8.9 47V39E (GTG→GAG) ‡PP_2417iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (24/17);  new base T (3/3);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

AACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGT  >  NC_002947/2763648‑2763939
                                                                                                                                                   |                                                                                                                                                
aaCATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCAc                                                                                                                                                 <  7:26600/149‑1 (MQ=255)
                tGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAc                                                                                                                                     >  1:136816/1‑145 (MQ=255)
                tGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAc                                                                                                                                     <  2:136816/145‑1 (MQ=255)
                 gACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTgg                                                                                                                                >  4:39373/1‑149 (MQ=255)
                      cacaGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGa                                                                                                                            >  1:2890/1‑148 (MQ=255)
                        caGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAg                                                                                                                         >  7:80197/1‑149 (MQ=255)
                                  gCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCg                                                                                                               <  3:39160/149‑1 (MQ=255)
                                       gcgcCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAAt                                                                                                          >  2:16120/1‑149 (MQ=255)
                                           cacaAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCt                                                                                                                                               <  3:280061/108‑1 (MQ=255)
                                           cacaAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCt                                                                                                                                               >  4:280061/1‑108 (MQ=255)
                                            acaAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCgg                                                                                                     >  3:5450/1‑149 (MQ=255)
                                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                   >  4:25876/1‑109 (MQ=38)
                                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                   >  4:21954/1‑109 (MQ=38)
                                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                   <  3:25876/111‑3 (MQ=38)
                                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                   <  3:21954/111‑3 (MQ=38)
                                                            aGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGggc                                                                                     >  5:145170/1‑149 (MQ=255)
                                                                     gTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCaa                                                                               >  4:264701/1‑146 (MQ=255)
                                                                         ggCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCg                                                                        >  4:155086/1‑149 (MQ=255)
                                                                          gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                                       >  8:172373/1‑149 (MQ=255)
                                                                          gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                                       >  7:32094/1‑149 (MQ=255)
                                                                          gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                                       >  2:286769/1‑149 (MQ=255)
                                                                             gTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTGCCGGCCAGCAGGGAGGCCAACAGAGCGGc                                                                      >  8:293038/1‑147 (MQ=255)
                                                                              tAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGcc                                                                                 <  2:42827/135‑1 (MQ=255)
                                                                              tAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGATGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGcc                                                                                 >  1:42827/1‑135 (MQ=255)
                                                                                gTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAggg                                                                 >  7:118245/1‑149 (MQ=255)
                                                                                gTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAgg                                                                  <  7:172373/148‑1 (MQ=255)
                                                                                      gACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACcg                                                           <  3:92667/149‑1 (MQ=255)
                                                                                        cGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACcgcg                                                         <  1:286769/149‑1 (MQ=255)
                                                                                               ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCaa                                                                                                           <  1:143849/92‑1 (MQ=255)
                                                                                               ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCaa                                                                                                           >  2:143849/1‑92 (MQ=255)
                                                                                               ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAgg                                                  <  6:145170/149‑1 (MQ=255)
                                                                                                 cGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGt                                                <  4:5450/149‑1 (MQ=255)
                                                                                                       tCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAgg                                          <  3:155086/149‑1 (MQ=255)
                                                                                                         gggTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAggag                                        >  8:9778/1‑147 (MQ=255)
                                                                                                          ggTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCgg                                       <  8:118245/149‑1 (MQ=255)
                                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                                   >  4:128915/1‑149 (MQ=255)
                                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                                   >  3:279114/1‑149 (MQ=255)
                                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                                   >  3:157527/1‑149 (MQ=255)
                                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatc                                    <  2:2890/148‑1 (MQ=255)
                                                                                                               aCCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatcat                                  >  7:265653/1‑149 (MQ=255)
                                                                                                                 cGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGAtcatca                                >  3:11592/1‑149 (MQ=255)
                                                                                                                        gcggcTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGgct                         >  6:55416/1‑149 (MQ=255)
                                                                                                                                ccACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTAc                  <  8:80197/148‑1 (MQ=255)
                                                                                                                                 cacaGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCg                >  1:85736/1‑149 (MQ=255)
                                                                                                                                      gtggtTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGc           <  3:39373/149‑1 (MQ=255)
                                                                                                                                       tggtTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCg          <  8:265653/149‑1 (MQ=255)
                                                                                                                                               cAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGt  <  4:279114/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AACATCGCCTGCAGGATGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGT  >  NC_002947/2763648‑2763939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑