Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,763,803 G→A 13.3% V36V (GTC→GTT)  PP_2417 ← iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,763,8030GA13.3% 111.8 / 7.8 45V36V (GTC→GTT) PP_2417iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (23/16);  new base A (3/3);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGTGGACAGCGTCTG  >  NC_002947/2763664‑2763951
                                                                                                                                           |                                                                                                                                                    
tGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAc                                                                                                                                                 >  1:136816/1‑145 (MQ=255)
tGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAc                                                                                                                                                 <  2:136816/145‑1 (MQ=255)
 gACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTgg                                                                                                                                            >  4:39373/1‑149 (MQ=255)
      cacaGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGa                                                                                                                                        >  1:2890/1‑148 (MQ=255)
        caGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAg                                                                                                                                     >  7:80197/1‑149 (MQ=255)
                  gCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCg                                                                                                                           <  3:39160/149‑1 (MQ=255)
                       gcgcCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAAt                                                                                                                      >  2:16120/1‑149 (MQ=255)
                            acaAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCgg                                                                                                                 >  3:5450/1‑149 (MQ=255)
                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                               >  4:21954/1‑109 (MQ=38)
                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                               >  4:25876/1‑109 (MQ=38)
                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                               <  3:25876/111‑3 (MQ=38)
                                    ccGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTCGGTCAGGTGCTGGCCAACCAcct                                                                                                                                               <  3:21954/111‑3 (MQ=38)
                                            aGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGggc                                                                                                 >  5:145170/1‑149 (MQ=255)
                                                     gTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCaa                                                                                           >  4:264701/1‑146 (MQ=255)
                                                         ggCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCg                                                                                    >  4:155086/1‑149 (MQ=255)
                                                          gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                                                   >  7:32094/1‑149 (MQ=255)
                                                          gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                                                   >  2:286769/1‑149 (MQ=255)
                                                          gCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCgg                                                                                   >  8:172373/1‑149 (MQ=255)
                                                             gTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTGCCGGCCAGCAGGGAGGCCAACAGAGCGGc                                                                                  >  8:293038/1‑147 (MQ=255)
                                                              tAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGcc                                                                                             <  2:42827/135‑1 (MQ=255)
                                                              tAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGATGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGcc                                                                                             >  1:42827/1‑135 (MQ=255)
                                                                gTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAggg                                                                             >  7:118245/1‑149 (MQ=255)
                                                                gTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAgg                                                                              <  7:172373/148‑1 (MQ=255)
                                                                      gACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACcg                                                                       <  3:92667/149‑1 (MQ=255)
                                                                        cGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACcgcg                                                                     <  1:286769/149‑1 (MQ=255)
                                                                               ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCaa                                                                                                                       <  1:143849/92‑1 (MQ=255)
                                                                               ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCaa                                                                                                                       >  2:143849/1‑92 (MQ=255)
                                                                               ggCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAgg                                                              <  6:145170/149‑1 (MQ=255)
                                                                                 cGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGt                                                            <  4:5450/149‑1 (MQ=255)
                                                                                       tCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAgg                                                      <  3:155086/149‑1 (MQ=255)
                                                                                         gggTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAggag                                                    >  8:9778/1‑147 (MQ=255)
                                                                                          ggTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCgg                                                   <  8:118245/149‑1 (MQ=255)
                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                                               >  4:128915/1‑149 (MQ=255)
                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                                               >  3:157527/1‑149 (MQ=255)
                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatca                                               >  3:279114/1‑149 (MQ=255)
                                                                                              cACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatc                                                <  2:2890/148‑1 (MQ=255)
                                                                                               aCCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGatcat                                              >  7:265653/1‑149 (MQ=255)
                                                                                                 cGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGAtcatca                                            >  3:11592/1‑149 (MQ=255)
                                                                                                        gcggcTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGgct                                     >  6:55416/1‑149 (MQ=255)
                                                                                                                ccACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTAc                              <  8:80197/148‑1 (MQ=255)
                                                                                                                 cacaGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCg                            >  1:85736/1‑149 (MQ=255)
                                                                                                                      gtggtTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGc                       <  3:39373/149‑1 (MQ=255)
                                                                                                                       tggtTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCg                      <  8:265653/149‑1 (MQ=255)
                                                                                                                               cAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGt              <  4:279114/149‑1 (MQ=255)
                                                                                                                                    ccTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGTGGACa         <  4:157527/149‑1 (MQ=255)
                                                                                                                                       gaccgaccACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGTGGACAGCg      >  6:136777/1‑149 (MQ=255)
                                                                                                                                          cgaccACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGTGGACAGCGTCt   <  1:16120/149‑1 (MQ=255)
                                                                                                                                           gaccACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGTGGACAGCGTCTg  >  7:28698/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
TGACACCACAGGTGGCCAGCCCGGCGCCACAAGCGGCCGCGACCAGGGTGCGGGTCAGGCGGTAGTTCCAGACGATGCCGGCGTCGATCGGGTCCACCGGGTAGGCGGCTTGCCACAGGTGGTTGGCCAGCACCTGACCGACCACGCTGGGCGACAGGTTGGTTTCGCCAATGGCGGTACCGGCCAGCAGGGCGGCCAACAGAGCGGCCAGGGCAACCGCGATGCAGGGTAACAGGCGGATCATCACGGGGCTGCTTTACCGCTGGCGAAGGCCGTGGACAGCGTCTG  >  NC_002947/2763664‑2763951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑