Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,787,623 G→A 11.6% intergenic (+70/‑55) ahpC → / → ahpF peroxiredoxin/alkylhydroperoxide reductase small subunit/alkyl hydroperoxide reductase subunit F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,787,6230GA11.6% 108.2 / 5.3 43intergenic (+70/‑55)ahpC/ahpFperoxiredoxin/alkylhydroperoxide reductase small subunit/alkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base A (3/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.51e-01

GGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGC  >  NC_002947/2787476‑2787769
                                                                                                                                                   |                                                                                                                                                  
ggCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCgg                                                                                                                                                   <  4:299056/149‑1 (MQ=255)
ggCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCgg                                                                                                                                                   <  8:263753/149‑1 (MQ=255)
 gCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCggg                                                                                                                                                  >  4:205232/1‑149 (MQ=255)
  cGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGc                                                                                                                                                 <  2:74206/149‑1 (MQ=255)
              ggCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTgg                                                                                                                                     >  8:107980/1‑149 (MQ=255)
                ccAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGc                                                                                                                                   >  8:241863/1‑149 (MQ=255)
                  aaGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGCGt                                                                                                                                 <  6:293269/149‑1 (MQ=255)
                      ggAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGtttta                                                                                                                             >  8:285650/1‑148 (MQ=255)
                          agaagGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTAt                                                                                                                         >  3:272690/1‑149 (MQ=255)
                           gaagGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATc                                                                                                                        <  3:56263/149‑1 (MQ=255)
                            aagGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATACCGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGttttacggggc                                                                                                                       <  7:285650/149‑8 (MQ=255)
                               gCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGCGtt                                                                                                                                >  3:284582/1‑137 (MQ=255)
                               gCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGCGtt                                                                                                                                <  4:284582/137‑1 (MQ=255)
                                  aaGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGttt                                                                                                                 <  3:205232/149‑1 (MQ=255)
                                      cACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCAGGGCGTTTTTGTATCCGAGTTTTGa                                                                                                              >  6:194137/1‑148 (MQ=255)
                                       aCTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGaa                                                                                                             >  1:294807/1‑148 (MQ=255)
                                                  gTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGcc                                                                                                 <  7:107980/149‑1 (MQ=255)
                                                   tCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGccc                                                                                                <  5:206008/149‑1 (MQ=255)
                                                     gCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCg                                                                                               <  1:106729/148‑1 (MQ=255)
                                                         gACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGt                                                                                          >  1:220719/1‑149 (MQ=255)
                                                         gACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGt                                                                                          <  7:91585/149‑1 (MQ=255)
                                                          aCCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGtt                                                                                         <  2:220719/149‑1 (MQ=255)
                                                           ccTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTg                                                                                        >  8:281117/1‑149 (MQ=255)
                                                            cTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTgg                                                                                       <  2:6640/149‑1 (MQ=255)
                                                              cGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGCCCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGAc                                                                                     >  1:206954/1‑149 (MQ=255)
                                                               gTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGa                                                                                      <  2:199010/147‑1 (MQ=255)
                                                               gTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGa                                                                                      <  5:194137/147‑1 (MQ=255)
                                                                tAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGc                                                                                   >  5:173729/1‑149 (MQ=255)
                                                                           tAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTgg                                                                                                                                     <  7:145243/88‑1 (MQ=255)
                                                                           tAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTgg                                                                                                                                     >  8:145243/1‑88 (MQ=255)
                                                                                                   ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGc                                                <  7:281117/149‑1 (MQ=255)
                                                                                                     tAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCAc                                                                                >  7:188590/1‑115 (MQ=255)
                                                                                                     tAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCAc                                                                                <  8:188590/115‑1 (MQ=255)
                                                                                                       agcagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCggg                                             <  2:206954/148‑1 (MQ=255)
                                                                                                        gcagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTc                                           >  5:17025/1‑149 (MQ=255)
                                                                                                         cagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCa                                          >  4:69686/1‑149 (MQ=255)
                                                                                                               ccAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGCATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGc                                    >  6:199086/1‑149 (MQ=255)
                                                                                                                   aGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGACCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGAt                                >  4:256216/1‑149 (MQ=255)
                                                                                                                     cTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCg                              <  6:17025/149‑1 (MQ=255)
                                                                                                                          gTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCtt                                                                            <  4:74862/98‑1 (MQ=255)
                                                                                                                          gTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCtt                                                                            >  3:74862/1‑98 (MQ=255)
                                                                                                                          gTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATc                         <  2:294807/149‑1 (MQ=255)
                                                                                                                                       acaaaaaCGCCCAGGCGAGGTCGCCCGGGCGTTTTTGTATGCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTc             >  1:147753/5‑148 (MQ=255)
                                                                                                                                             aCGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGacgacg      <  7:241863/149‑1 (MQ=255)
                                                                                                                                               gCCCAGGCGAGGTCGCCCGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGgc    >  8:201518/1‑149 (MQ=255)
                                                                                                                                                 ccGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGgcgc  >  8:243703/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGC  >  NC_002947/2787476‑2787769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑