Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,802,588 C→T 8.7% G324G (GGC→GGT)  rbsA‑I → ribose ABC transporter ‑ ATP‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,802,5880CT 8.7% 130.6 / 2.4 46G324G (GGC→GGT) rbsA‑Iribose ABC transporter ‑ ATP‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (22/20);  new base T (2/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTTGGGCAACCTGGGCGCGGTAT  >  NC_002947/2802441‑2802736
                                                                                                                                                   |                                                                                                                                                    
ccGGGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGCGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGcc                                                                                                                                                     <  8:112614/149‑1 (MQ=255)
   tGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAgc                                                                                                                                                  >  1:158356/1‑149 (MQ=255)
      cGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAgccgc                                                                                                                                               <  2:95841/149‑1 (MQ=255)
         cAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCcac                                                                                                                                            <  8:85156/149‑1 (MQ=255)
                   gAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTAAGGTGACGATCGCGCCGACAGCGGCGGTATCCCGCTCGGCCAGCCGCCACAGGCGGTCAg                                                                                                                                  >  5:187649/1‑149 (MQ=255)
                        gtCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATc                                                                                                                              >  5:312760/1‑148 (MQ=255)
                         tCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAg                                                                                                                                  <  2:141690/143‑1 (MQ=255)
                         tCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAg                                                                                                                                  >  1:141690/1‑143 (MQ=255)
                           gTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACt                                                                                                                          >  8:637/1‑149 (MQ=255)
                               gAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGc                                                                                                                       <  2:158356/148‑1 (MQ=255)
                                aaGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGTCAGCCGCCACAGGCGGTCAGCATCGACTCGcc                                                                                                                      >  6:22838/1‑148 (MQ=255)
                                aaGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGTCAGCCGCCACAGGCGGTCAGCATCGACTCGcc                                                                                                                      >  6:18321/1‑148 (MQ=255)
                                       ggcagggGAGTTTTTTGGGATCTCGGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCTCTCGGCCATCCGCCACAGGCGGTCAGCATCGACTCGCCCAAgg                                                                                                                 <  6:184900/146‑1 (MQ=255)
                                           ggggAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAgt                                                                                                          >  1:205354/1‑149 (MQ=255)
                                              gagaTTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAgtgcg                                                                                                       >  8:141327/1‑149 (MQ=255)
                                                 aTTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAgtgcgtgc                                                                                                    >  1:78271/1‑149 (MQ=255)
                                                   ttttGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACCGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGGCg                                                                                                  >  5:89099/1‑149 (MQ=255)
                                                    tttGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCg                                                                                                  <  5:279193/148‑1 (MQ=255)
                                                          aTCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATcgc                                                                                           >  8:186709/1‑149 (MQ=255)
                                                                ggCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGAt                                                                                     <  7:141327/149‑1 (MQ=255)
                                                                     gATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAAcc                                                                                 >  3:30042/1‑148 (MQ=255)
                                                                            gccggccgTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCg                                                                         >  7:52831/1‑149 (MQ=255)
                                                                                 ccgTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAg                                                                    <  4:5399/149‑1 (MQ=255)
                                                                                 ccgTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAg                                                                    >  7:149754/1‑149 (MQ=255)
                                                                                  cgTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGTCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAgg                                                                   <  5:22838/149‑1 (MQ=255)
                                                                                  cgTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGTCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAgg                                                                   <  5:18321/149‑1 (MQ=255)
                                                                                            tgctgcGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCctg                                                         <  8:149754/149‑1 (MQ=255)
                                                                                              ctgcGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCctgct                                                       >  5:125992/1‑149 (MQ=255)
                                                                                              ctgcGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCctgct                                                       <  4:30042/149‑1 (MQ=255)
                                                                                                 cgcCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCctgctg                                                      <  6:187649/147‑1 (MQ=255)
                                                                                                 cgcCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGtt                                                    <  2:78271/149‑1 (MQ=255)
                                                                                                   ccTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGa                                                  <  6:312760/149‑1 (MQ=255)
                                                                                                    cTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGAc                                                 <  6:125992/149‑1 (MQ=255)
                                                                                                    cTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGAc                                                 <  8:188984/149‑1 (MQ=255)
                                                                                                       aTCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACg                                                >  7:59541/1‑147 (MQ=255)
                                                                                                       aTCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACg                                                >  8:13185/1‑147 (MQ=255)
                                                                                                            cGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGt                                            <  2:205354/146‑1 (MQ=255)
                                                                                                              gtcccGATCGCGCCGACCGCGGCGGGATCGCGCTCGGCCAGCCGCCACAGGCGGGCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATc                                       <  3:249767/146‑1 (MQ=255)
                                                                                                               tGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                                        <  8:55399/99‑1 (MQ=255)
                                                                                                               tGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCt                                                                                        >  7:55399/1‑99 (MQ=255)
                                                                                                               tGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGAt                                        >  8:7280/1‑147 (MQ=255)
                                                                                                                 ccGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCgcgc                                                                                         >  6:13526/1‑96 (MQ=255)
                                                                                                                 ccGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCgcgc                                                                                         <  5:13526/96‑1 (MQ=255)
                                                                                                                    aTCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGcc                                 <  7:13185/149‑1 (MQ=255)
                                                                                                                                            cgcTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTTGGGCAACCTGGgc         >  2:28182/1‑149 (MQ=255)
                                                                                                                                               tCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTTGGGCAACCTGGGcgcg      >  8:170778/1‑149 (MQ=255)
                                                                                                                                                 ggCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTTGGGCAACCTGGGCGCgg     >  8:176207/1‑148 (MQ=255)
                                                                                                                                                   ccAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGtt                                                    <  1:79211/99‑1 (MQ=255)
                                                                                                                                                   ccAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGtt                                                    >  2:79211/1‑99 (MQ=255)
                                                                                                                                                   ccAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTTGGGCAACCTGGGCGCGGTAt  >  4:106697/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGTGGCGACAAGGTGCGCGAGGTGTCGTTCGAAGTCAGGGCAGGGGAGATTTTTGGCATCTCCGGCCTGATCGGCGCCGGCCGTACCGAGCTGCTGCGCCTGATCTACGGTGCCGATCGCGCCGACAGCGGCGGTATCGCGCTCGGCCAGCCGCCACAGGCGGTCAGCATCGACTCGCCCAAGGCTGCAGTGCGTGCCGGTATCGCGCTGATAACCGAAGACCGCAAAGGCGAGGGCCTGCTGTTGACGCAGTCGATCAGCGCCAACATTGCGTTGGGCAACCTGGGCGCGGTAT  >  NC_002947/2802441‑2802736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑