Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,810,387 G→A 8.1% W265* (TGG→TAG)  thrS → threonine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,810,3870GA 8.1% 138.5 / 2.3 49W265* (TGG→TAG) thrSthreonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01

CGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCC  >  NC_002947/2810239‑2810532
                                                                                                                                                    |                                                                                                                                                  
cgcgTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTg                                                                                                                                                    <  2:292/149‑1 (MQ=255)
  cgTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCTGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGc                                                                                                                                                  <  6:206670/149‑1 (MQ=255)
       acggcacggcCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGc                                                                                                                                              >  6:307018/1‑148 (MQ=255)
                ccTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTAGCACGCCAACGGC‑Tgg                                                                                                                                   >  7:25736/1‑149 (MQ=255)
                ccTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTAGCACGCCAACGGC‑Tg                                                                                                                                    >  7:30430/1‑148 (MQ=255)
                    ggCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCaa                                                                                                                                           <  3:141326/138‑1 (MQ=255)
                    ggCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCaa                                                                                                                                           >  4:141326/1‑138 (MQ=255)
                      cTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTc                                                                                                                             <  4:89204/149‑1 (MQ=255)
                             gaagCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGAAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAgg                                                                                                                      <  3:88034/149‑1 (MQ=255)
                                       gctgCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGc                                                                                                             <  5:307018/148‑1 (MQ=255)
                                           cGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTa                                                                                                         >  5:104109/1‑148 (MQ=255)
                                                  ccAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATgcgtg                                                                                                 >  6:8310/1‑149 (MQ=255)
                                                         aTCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTa                                                                                                         <  1:200742/134‑1 (MQ=255)
                                                         aTCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTa                                                                                                         >  2:200742/1‑134 (MQ=255)
                                                             aagaagCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAg                                                                                                              <  3:143555/125‑1 (MQ=255)
                                                             aagaagCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAg                                                                                                              >  4:143555/1‑125 (MQ=255)
                                                             aagaagCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAgcgc                                                                                      >  5:261324/1‑149 (MQ=255)
                                                                  gCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGaaaa                                                                                 >  2:236995/1‑149 (MQ=255)
                                                                    cGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAAc                                                                                <  7:187414/148‑1 (MQ=255)
                                                                      aaaaaCGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTAGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAATCGGc                                                                             <  8:30430/149‑1 (MQ=255)
                                                                      aaaaaCGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTAGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAATCGGc                                                                             <  8:25736/149‑1 (MQ=255)
                                                                            gcgACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGa                                                                                    <  3:193955/136‑1 (MQ=255)
                                                                            gcgACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGa                                                                                    >  4:193955/1‑136 (MQ=255)
                                                                                   ccGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTTGCGTGCAGCGCGAAAACGGCTACCAGGAAATc                                                                 >  5:21567/1‑148 (MQ=255)
                                                                                   ccGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATc                                                                 >  8:217010/1‑148 (MQ=255)
                                                                                    cGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATc                                                                 >  8:167067/1‑147 (MQ=255)
                                                                                    cGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCaa                                                               <  6:127436/149‑1 (MQ=255)
                                                                                      cAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGa                                                             >  7:7253/1‑149 (MQ=255)
                                                                                         gATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGAccc                                                          <  4:273295/149‑1 (MQ=255)
                                                                                             ggCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCg                                                        >  5:270175/1‑147 (MQ=255)
                                                                                                   cagcTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGt                                                >  4:265716/1‑149 (MQ=255)
                                                                                                    agcTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTc                                               <  7:283956/149‑1 (MQ=255)
                                                                                                      cTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTc                                                                                                                 <  5:266771/81‑1 (MQ=255)
                                                                                                      cTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTc                                                                                                                 >  6:266771/1‑81 (MQ=255)
                                                                                                       gcGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGGCGACCAGATACGCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAAGCCAGCCCCCGCAGGGTGTCgcc                                            >  6:244943/2‑147 (MQ=255)
                                                                                                             tGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATc                                      >  4:83686/1‑149 (MQ=255)
                                                                                                                   aCCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTgg                                <  2:191954/149‑1 (MQ=255)
                                                                                                                     cTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCCGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGa                              <  7:217010/149‑1 (MQ=255)
                                                                                                                        cAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGc                            <  5:8310/148‑1 (MQ=255)
                                                                                                                          ggAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGtt                         >  1:182865/1‑149 (MQ=255)
                                                                                                                           gaagaaGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTc                        >  3:202714/1‑149 (MQ=255)
                                                                                                                              gaagCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGc                                                       >  3:12217/1‑115 (MQ=255)
                                                                                                                              gaagCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGc                                                       <  4:12217/115‑1 (MQ=255)
                                                                                                                                  ccccGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCAc                 <  2:53036/149‑1 (MQ=255)
                                                                                                                                  ccccGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCAc                 <  4:168266/149‑1 (MQ=255)
                                                                                                                                    ccGGGCATGGTGTTCTGGCACGCCAACGGCTTGGACCGTCTACAAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAATACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGcaaat                >  1:184739/1‑145 (MQ=255)
                                                                                                                                           tGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAAc        <  4:202714/149‑1 (MQ=255)
                                                                                                                                                  cTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGcc  <  6:270175/148‑1 (MQ=255)
                                                                                                                                                  cTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGcc  <  6:261324/148‑1 (MQ=255)
                                                                                                                                                  cTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGcc  <  3:83686/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCGTGTACGGCACGGCCTGGGCTGACAAGAAGCAGCTGGCTGCGTACATCCAGCGCATCGAAGAAGCCGAAAAACGCGACCACCGCAAGATCGGCAAGCAGCTCGACCTGTTCCACCTGCAGGAAGAAGCCCCGGGCATGGTGTTCTGGCACGCCAACGGC‑TGGACCGTCTACCAGGTACTCGAGCAGTACATGCGTGGCGTGCAGCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCC  >  NC_002947/2810239‑2810532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑