Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,831,283 T→C 7.2% Y83C (TAC→TGC)  PP_2484 ← ArsR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,831,2830TC 7.2% 155.2 / 2.1 55Y83C (TAC→TGC) PP_2484ArsR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base C (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGACCTCCTG  >  NC_002947/2831136‑2831431
                                                                                                                                                   |                                                                                                                                                    
cTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgta                                                                                                                                                     <  4:139433/149‑1 (MQ=255)
 ttCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgtag                                                                                                                                                    >  4:165369/1‑149 (MQ=255)
          tctgatcGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCa                                                                                                                                            <  8:141358/148‑1 (MQ=255)
            tgatcGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTAAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCATTGAAGAAGTCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAggg                                                                                                                                         >  1:120207/1‑149 (MQ=255)
             gatcGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGtagtag                                                                                                                                                 >  5:197852/1‑140 (MQ=255)
             gatcGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGtagtag                                                                                                                                                 <  6:197852/140‑1 (MQ=255)
              atcGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAgg                                                                                                                                          <  7:22189/146‑1 (MQ=255)
                     tttGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCAGATCCg                                                                                                                                >  5:21301/1‑149 (MQ=255)
                        ggTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCt                                                                                                                              <  3:165369/148‑1 (MQ=255)
                           cAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGa                                                                                                                          >  7:268400/1‑149 (MQ=255)
                            aGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgtagt                                                                                                                                                   <  1:265288/123‑1 (MQ=255)
                            aGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTgtagt                                                                                                                                                   >  2:265288/1‑123 (MQ=255)
                                    gTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCaa                                                                                                                 >  1:129120/1‑149 (MQ=255)
                                           aCATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAAtt                                                                                                               <  5:191744/144‑1 (MQ=255)
                                           aCATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAAtt                                                                                                               >  6:191744/1‑144 (MQ=255)
                                           aCATCGGCAAAGACTGAAATCAGCCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgcgc                                                                                                           <  1:152603/148‑1 (MQ=255)
                                               cGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCt                                                                                                      >  6:273288/1‑149 (MQ=255)
                                                 gCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAAtt                                                                                                               >  8:174362/1‑138 (MQ=255)
                                                 gCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAAtt                                                                                                               <  7:174362/138‑1 (MQ=255)
                                                 gCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGCAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTg                                                                                                     >  1:265132/1‑148 (MQ=255)
                                                 gCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGCAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTg                                                                                                     >  1:261015/1‑148 (MQ=255)
                                                  cAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTa                                                                                                   <  8:268400/149‑1 (MQ=255)
                                                   aaaGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgcgc                                                                                                           <  4:298112/140‑1 (MQ=255)
                                                      gACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGc                                                                                               >  6:142951/1‑149 (MQ=255)
                                                           aaaTCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGc                                                                                            >  6:283206/1‑147 (MQ=255)
                                                           aaaTCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGc                                                                                            >  1:30578/1‑147 (MQ=255)
                                                                    agAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgcgc                                                                                                           >  7:272710/1‑123 (MQ=255)
                                                                    agAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgcgc                                                                                                           <  8:272710/123‑1 (MQ=255)
                                                                    agAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGc                                                                                  >  2:14563/1‑148 (MQ=255)
                                                                    agAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGAAGCGTTTGTAGTATGTACAGGGGCCGATACGCTTCGATTTAACCAATTTCGCGCGCTGTATAGCGTCGAGATAAAGc                                                                                  >  1:94734/1‑148 (MQ=255)
                                                                     gAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGa                                                                                <  1:14563/149‑1 (MQ=255)
                                                                      aaaTACAAGGGTTGCAGCCCTAGATTCTTCCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGAt                                                                               <  5:273288/149‑1 (MQ=255)
                                                                       aaTACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTTCGAGATAAAGCGAt                                                                               <  2:159739/148‑1 (MQ=255)
                                                                            aaGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGtt                                                                         >  7:225255/1‑149 (MQ=255)
                                                                              gggTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTg                                                                                                                             >  2:85044/1‑95 (MQ=255)
                                                                              gggTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTg                                                                                                                             <  1:85044/95‑1 (MQ=255)
                                                                                   gCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgcgcgcg                                                                                                        <  8:104594/111‑1 (MQ=255)
                                                                                   gCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgcgcgcg                                                                                                        >  7:104594/1‑111 (MQ=255)
                                                                                    cAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCg                                                                  <  2:129120/148‑1 (MQ=255)
                                                                                        ccTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTgcgcgcgc                                                                                                       <  2:1070/107‑1 (MQ=255)
                                                                                                ctttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGCAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTgc                                                      <  2:261015/148‑1 (MQ=255)
                                                                                                ctttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGCAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTgc                                                      <  2:265132/148‑1 (MQ=255)
                                                                                                  ttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAggg                                                                                                                                         <  5:363/63‑1 (MQ=255)
                                                                                                  ttcttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAggg                                                                                                                                         >  6:363/1‑63 (MQ=255)
                                                                                                     ttGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCtt                                                >  6:42941/1‑149 (MQ=255)
                                                                                                        cAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGga                                             <  5:142951/149‑1 (MQ=255)
                                                                                                                  aagaagGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGAc                                   <  5:283206/149‑1 (MQ=255)
                                                                                                                  aagaagGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTACGCTCTTGGATGATACTGAc                                   <  8:225255/149‑1 (MQ=255)
                                                                                                                         cATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGc                                                                                  <  1:51577/95‑1 (MQ=255)
                                                                                                                         cATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGc                                                                                  >  2:51577/1‑95 (MQ=255)
                                                                                                                         cATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGTGAGAGGCCCGTGCGCTCTTGGATGATACTGACGcacaca                            >  1:126035/1‑149 (MQ=255)
                                                                                                                          aTCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCacacac                           <  5:42941/149‑1 (MQ=255)
                                                                                                                          aTCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCacacac                           >  1:134744/1‑149 (MQ=255)
                                                                                                                          aTCGATATTCCCATCGAGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCCCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCacacac                           >  2:180217/1‑149 (MQ=255)
                                                                                                                              atatTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTAc                       >  8:172564/1‑149 (MQ=255)
                                                                                                                              atatTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTAc                       <  2:30578/149‑1 (MQ=255)
                                                                                                                                    ccATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGttt                  <  6:21301/148‑1 (MQ=255)
                                                                                                                                     cATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCt                <  1:180217/149‑1 (MQ=255)
                                                                                                                                         gTGGCGTTTGTAGTATGTCCAGGGGCCGATCCGCTGCGATGTCAGCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGt            <  8:106779/149‑1 (MQ=255)
                                                                                                                                            gCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGa         >  7:603/1‑149 (MQ=255)
                                                                                                                                                ttGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGAcctc     >  7:128928/1‑149 (MQ=255)
                                                                                                                                                   tagtagGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTCCCGTTTCTGGGTCGACCTCCTg  >  2:264717/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTTCGGATGATCTGATCGTTATTTGGTCAGATTGCTGTTTTTGACATCGGCAAAGACTGAAATCAGTCAGAAATACAAGGGTTGCAGCCCTAGATTCTTTCTTGCAGCGCCTTGAAGAAGGCATCGATATTCCCATCGTGGCGTTTGTAGTAGGTCCAGGGGCCGATCCGCTGCGATGTCACCAATTGCGCGCGCTGTAGAGCTGCGAGATAAAGCGATGTCGTTGACTGCGAGAGGCCCGTGCGCTCTTGGATGATACTGACGCACACACCTACCGTTTCTGGGTCGACCTCCTG  >  NC_002947/2831136‑2831431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑