Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,839,676 A→T 7.9% A73A (GCT→GCA)  PP_5529 ← epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,839,6760AT 7.9% 187.9 / 3.8 63A73A (GCT→GCA) PP_5529epimerase
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base T (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TTGTTCCAGTGCAATGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGCGGCAGGCG  >  NC_002947/2839529‑2839821
                                                                                                                                                   |                                                                                                                                                 
ttGTTCCAGTGCAATGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAg                                                                                                                                                  <  4:274456/149‑1 (MQ=255)
   ttCCAGTGCAATGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCg                                                                                                                                               >  1:96527/1‑149 (MQ=255)
      cAGTGCAATGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTATGccagcc                                                                                                                                            <  2:6067/149‑5 (MQ=255)
       aGTGCAATGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCt                                                                                                                                           >  1:251350/1‑149 (MQ=255)
       aGTGCAATGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCt                                                                                                                                           >  5:124464/1‑149 (MQ=255)
            aaTGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTg                                                                                                                                          <  5:126807/145‑1 (MQ=255)
            aaTGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTg                                                                                                                                          >  6:126807/1‑145 (MQ=255)
                     cacaAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTATGCCAGCCCGGCTTACCAGGcgg                                                                                                                             <  1:59061/149‑3 (MQ=255)
                     cacaAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTc                                                                                                                             >  7:77786/1‑149 (MQ=255)
                             gagaTATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCt                                                                                                                     <  2:251350/149‑1 (MQ=255)
                                 tataCGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTc                                                                                                                  <  1:278825/148‑1 (MQ=255)
                                          aTAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTc                                                                                                          <  2:188101/147‑1 (MQ=255)
                                            aaCGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTgg                                                                                                                                         <  5:302445/114‑1 (MQ=255)
                                            aaCGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTgg                                                                                                                                         >  6:302445/1‑114 (MQ=255)
                                            aaCGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTc                                                                                                                  <  6:25733/137‑1 (MQ=255)
                                            aaCGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTc                                                                                                                  >  5:25733/1‑137 (MQ=255)
                                             aCGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAAc                                                                                                     >  7:110255/1‑149 (MQ=255)
                                                  ttGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAg                                                                                                 >  7:34535/1‑148 (MQ=255)
                                                    gtgCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGaa                                                                                               <  4:6275/148‑1 (MQ=255)
                                                        aCGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGAcc                                                                                          <  4:54031/149‑1 (MQ=255)
                                                         cgcgTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCt                                                                                                            <  5:67447/130‑1 (MQ=255)
                                                         cgcgTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCt                                                                                                            >  6:67447/1‑130 (MQ=255)
                                                          gcgTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCg                                                                                         >  4:140244/1‑148 (MQ=255)
                                                               tCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCt                                                                                   >  4:14491/1‑149 (MQ=255)
                                                                 aGTTAGGTGATGTGTCGGTGCGGTCGATTAGATAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGGTGGCTTCTCTTGGAATCGTAGACTGACCGGTccggcg                                                                                 >  8:157833/1‑145 (MQ=255)
                                                                    tAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCtcgat                                                                               <  8:77786/148‑1 (MQ=255)
                                                                       gTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCt                                                                                       <  8:59302/137‑1 (MQ=255)
                                                                       gTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCt                                                                                       >  7:59302/1‑137 (MQ=255)
                                                                         gATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATgg                                                                         >  4:191355/1‑149 (MQ=255)
                                                                          aTGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGa                                                                        <  3:85535/149‑1 (MQ=255)
                                                                            gtgtCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGAc                                                                       <  8:110255/148‑1 (MQ=255)
                                                                              gtCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCgg                                                                                                                                              >  5:139449/1‑75 (MQ=255)
                                                                              gtCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCgg                                                                                                                                              <  6:139449/75‑1 (MQ=255)
                                                                               tCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTc                                                                   <  8:34535/149‑1 (MQ=255)
                                                                                     ccTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAacac                                                             <  6:124464/149‑1 (MQ=255)
                                                                                       ttCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCt                                                           >  3:202626/1‑149 (MQ=255)
                                                                                       ttCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCt                                                           >  6:282127/1‑149 (MQ=255)
                                                                                         cGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTc                                                          <  2:96527/148‑1 (MQ=255)
                                                                                          gATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTc                                                          >  3:235434/1‑147 (MQ=255)
                                                                                           aTTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAg                                                       <  3:14491/149‑1 (MQ=255)
                                                                                              aGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCatat                                                                                                              >  1:155624/1‑91 (MQ=255)
                                                                                              aGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCatat                                                                                                              <  2:155624/91‑1 (MQ=255)
                                                                                               gAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGAcc                                                                                          <  2:35677/110‑1 (MQ=255)
                                                                                               gAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGAcc                                                                                          >  1:35677/1‑110 (MQ=255)
                                                                                                aaaaaCTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCACAGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTACAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCACTTGAc                                                  >  3:277640/1‑149 (MQ=255)
                                                                                                 ataaCTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCACGTCCTGGTAAGCCGGGCTGGCATACCAGTCTCGAGCGGCTGTCTTATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCTGTTGAcc                                                 <  4:277640/147‑1 (MQ=255)
                                                                                                     cTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGtt                                                     <  4:169380/141‑1 (MQ=255)
                                                                                                     cTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGtt                                                     >  3:169380/1‑141 (MQ=255)
                                                                                                      tCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAg                                             >  6:107117/1‑148 (MQ=255)
                                                                                                        ggTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGa                                            >  2:115443/1‑147 (MQ=255)
                                                                                                          tACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGc                                        <  2:171144/149‑1 (MQ=255)
                                                                                                               cGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGAAGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGAc                                   <  3:140244/149‑1 (MQ=255)
                                                                                                                   ccGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACgtgt                               <  7:157833/149‑1 (MQ=255)
                                                                                                                                gatttAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTCACCGTAGAAAGCCAGACGTGTGACGTCGTGccc                   >  4:56902/3‑148 (MQ=255)
                                                                                                                                  tttAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTtc                >  7:117871/1‑149 (MQ=255)
                                                                                                                                        gccgccTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGc          <  6:30154/149‑1 (MQ=255)
                                                                                                                                           gccTGGTATGCCAGCCCGGCTTACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGCgg        >  7:62539/1‑148 (MQ=255)
                                                                                                                                           gccTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGCgg        >  6:90762/1‑148 (MQ=255)
                                                                                                                                           gccTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGCGGc       <  5:90762/149‑1 (MQ=255)
                                                                                                                                            ccTGGTATGCCAGCCCGGCTTACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCt                                                                                   >  3:23693/1‑72 (MQ=25)
                                                                                                                                            ccTGGTATGCCAGCCCGGCTTACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCt                                                                                   <  4:23693/72‑1 (MQ=25)
                                                                                                                                                gTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGCGGCAgg    <  7:8406/147‑1 (MQ=255)
                                                                                                                                                gTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTACAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGCGGCAGgcg  >  2:274593/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTGTTCCAGTGCAATGACATGCACAAGCCGAGATATACGCTCATAACGACTTGTGCACGCGTGTCAGTTAGGTGATGTGTCGGTGCCTTCGATTAGAAAAACTCGGTACTCCGCACCGGAGAGACGATGCTTTAGCGCCGCCTGGTAAGCCGGGCTGGCATACCAGGCTCGAGCGGCTGTCATATCTTCGAAACGTAGAATGACCGCTCCCTCGATCGATGGACCTTCCAACACCTCCAGTTGACCGTAGAAAGCCAGACGTGTGACGTCGTGCCCTTCTCGTGCGGCAGGCG  >  NC_002947/2839529‑2839821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑