Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 68,598 T→G 8.3% P225P (CCA→CCC)  PP_0058 ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294768,5980TG 8.3% 204.2 / 8.0 71P225P (CCA→CCC) PP_00581‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (32/33);  new base G (4/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGT  >  NC_002947/68451‑68744
                                                                                                                                                   |                                                                                                                                                  
tAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGt                                                                                                                                                    <  5:308356/148‑1 (MQ=255)
  aTCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGc                                                                                                                                                 >  4:191714/1‑149 (MQ=255)
      aaaaaCTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCg                                                                                                                                             >  5:121355/1‑149 (MQ=255)
              cacacaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGt                                                                                                                                       <  2:219270/147‑1 (MQ=255)
                cacaAGTTCTCCACAGAAGGGCAATCCATGTGGTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGc                                                                                                                                   <  8:217009/149‑1 (MQ=255)
                 acaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCg                                                                                                                                  >  7:85831/1‑149 (MQ=255)
                 acaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCg                                                                                                                                  >  2:242752/1‑149 (MQ=255)
                 acaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTGCGGCAATGGCAGGTGGCACCGTGCCGTTGGCg                                                                                                                                  >  6:213630/1‑149 (MQ=255)
                 acaAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGAGGTCGTTGAGTTCGGCAATGGCACGTCGCCCCGTGCCGTTgg                                                                                                                                    <  5:213630/147‑1 (MQ=255)
                  caAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGt                                                                                                                                 <  8:85831/149‑1 (MQ=255)
                   aaGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTa                                                                                                                                <  6:121355/149‑1 (MQ=255)
                           cacaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGc                                                                                                                         >  7:65354/1‑148 (MQ=255)
                            acaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCt                                                                                                                        >  7:98597/1‑148 (MQ=255)
                            acaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTc                                                                                                                       >  8:142555/1‑149 (MQ=255)
                            acaGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTc                                                                                                                       >  2:91620/1‑149 (MQ=255)
                             caGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCa                                                                                                                      >  6:160553/1‑149 (MQ=255)
                               gAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGCCAACGGCACGGGGCCACGTGCCATTgcc                                                                                                                                   <  4:59420/134‑3 (MQ=255)
                               gAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGCCAACGGCACGGGGCCACGTGCCATTgcc                                                                                                                                   >  3:59420/1‑132 (MQ=255)
                                aaGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCAcc                                                                                                                    >  5:250838/1‑148 (MQ=255)
                                      aaGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTAc                                                                                                              <  4:141435/148‑1 (MQ=255)
                                          cATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACtt                                                                                                         <  3:191714/149‑1 (MQ=255)
                                           aTCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTc                                                                                                        >  3:140547/1‑149 (MQ=255)
                                           aTCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTACCAAGCTGTAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTc                                                                                                        >  2:289803/1‑149 (MQ=255)
                                                cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGc                                                                                                                                   <  8:167766/117‑1 (MQ=255)
                                                cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGc                                                                                                                                   >  7:167766/1‑117 (MQ=255)
                                                cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCg                                                                                                       <  1:242752/145‑1 (MQ=255)
                                                cTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGAtt                                                                                                    <  2:189923/148‑1 (MQ=255)
                                                   cTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGt                                                                                                  <  3:12326/147‑1 (MQ=255)
                                                    tGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACAt                                                                                                                             <  2:162758/119‑1 (MQ=255)
                                                    tGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTAcc                                                                                               >  4:117383/1‑149 (MQ=255)
                                                    tGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTAcc                                                                                               >  1:266372/1‑149 (MQ=255)
                                                     gAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCg                                                                                              >  5:237706/1‑149 (MQ=255)
                                                      aGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTTGCGTACATCGGATCACGAATTACCACTTAGATTGTAcctg                                                                                             >  6:289119/1‑147 (MQ=255)
                                                        gTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCa                                                                                           >  3:119555/1‑149 (MQ=255)
                                                               cGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGc                                                                                    <  4:232617/149‑1 (MQ=255)
                                                               cGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGc                                                                                    <  6:250838/149‑1 (MQ=255)
                                                                gCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTAc                                                                                                              <  4:239136/122‑1 (MQ=255)
                                                                gCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTAc                                                                                                              >  3:239136/1‑122 (MQ=255)
                                                                gCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGcc                                                                                   >  6:74895/1‑149 (MQ=255)
                                                                       gcggcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAAc                                                                              <  5:160553/147‑1 (MQ=255)
                                                                          gcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAACGGCACGGGGCCACGTGCCATTGCCga                                                                                                                                 >  4:59165/1‑92 (MQ=37)
                                                                          gcAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAACGGCACGGGGCCACGTGCCATTGCCga                                                                                                                                 <  3:59165/93‑2 (MQ=37)
                                                                              cGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTgg                                                                     <  4:30084/149‑1 (MQ=255)
                                                                              cGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTgg                                                                     <  4:119555/149‑1 (MQ=255)
                                                                              cGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTgg                                                                     <  6:200055/149‑1 (MQ=255)
                                                                              cGATCCCATGGCCCGCTGGGTCGCTTCGTACCAAGCTGTAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTgg                                                                     <  1:289803/149‑1 (MQ=255)
                                                                               gATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTggg                                                                    <  6:113902/149‑1 (MQ=255)
                                                                               gATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTgg                                                                     <  8:65354/148‑1 (MQ=255)
                                                                                aTCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGc                                                                   <  7:142555/149‑1 (MQ=255)
                                                                                    cATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAg                                                                >  1:115882/1‑148 (MQ=255)
                                                                                    cATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGa                                                               <  6:237706/149‑1 (MQ=255)
                                                                                      tGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGGGGACCGCGGCTCACCATATACCACTTCGATTTTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAAc                                                             >  1:254036/1‑149 (MQ=255)
                                                                                                     cTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTgg                                              <  8:24788/149‑1 (MQ=255)
                                                                                                        cGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgt                                                <  7:133127/144‑1 (MQ=255)
                                                                                                        cGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTtgt                                                >  8:133127/1‑144 (MQ=255)
                                                                                                           tCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTc                                                                                                                       <  8:54966/70‑1 (MQ=255)
                                                                                                           tCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTc                                                                                                                       >  7:54966/1‑70 (MQ=255)
                                                                                                            ccAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATc                                        <  8:98597/148‑1 (MQ=255)
                                                                                                             cAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCgg                                      <  2:115882/149‑1 (MQ=255)
                                                                                                                          ggTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTa                                                                                                 >  8:115874/1‑77 (MQ=255)
                                                                                                                          ggTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTa                                                                                                 <  7:115874/77‑1 (MQ=255)
                                                                                                                               ttGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACAACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCgg                     >  1:146170/1‑148 (MQ=255)
                                                                                                                                tGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCa                   >  4:5921/1‑149 (MQ=255)
                                                                                                                                  aGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCAt                  >  3:58373/1‑148 (MQ=255)
                                                                                                                                  aGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCAt                  >  2:31610/1‑148 (MQ=255)
                                                                                                                                   gTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTc                <  2:266372/149‑1 (MQ=255)
                                                                                                                                      cGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCAc              <  3:117383/148‑1 (MQ=255)
                                                                                                                                        ccAACGGCACGGGGCCACGTGCCATTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGc           >  5:102360/2‑149 (MQ=255)
                                                                                                                                           acggCACGGGGCCACGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGCCATTCACGGCCAg        >  5:209191/3‑149 (MQ=255)
                                                                                                                                              gCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGc     >  6:227862/1‑149 (MQ=255)
                                                                                                                                                aCGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCgg   >  1:195063/1‑149 (MQ=255)
                                                                                                                                                 cGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAACACCGGCAGCCCGGCATTCACGGCCAGGGCGGt  <  3:299481/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAATCCAAAAACTGCACACAAGTTATCCACAGAAGGTCAAGCCATCTGCTCCTGAGGTTTTTCCGCAGCTGGCGGCAGCGATCCCATGGCCCGCTGGGTCGCTTCGTTCCAAGCTGCAGCGCGGTCGTTGAGTTCGGCAATGGCACGTGGCCCCGTGCCGTTGGCGTACATCGGCTCACCAATTACCACTTCGATTGTACCGGCACGCTTGCCCCAACCGGTCTTGGGCCAGAACTTGCCGGCGTTGTGGGCAATCGGCAGCACCGGCAGCCCGGCATTCACGGCCAGGGCGGT  >  NC_002947/68451‑68744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑