Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 272,009 A→T 13.7% intergenic (+14/+15) PP_0218 → / ← metP sensory box protein/L,D‑methionine D‑methionine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947272,0090AT13.7% 121.6 / 11.5 51intergenic (+14/+15)PP_0218/metPsensory box protein/L,D‑methionine D‑methionine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base T (5/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

TGGGGATGCAGGTGCATGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATACCGATAGGCGA  >  NC_002947/271862‑272154
                                                                                                                                                   |                                                                                                                                                 
tGGGGATGCAGGTGCATGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACCGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAag                                                                                                                                                  <  5:206175/149‑1 (MQ=255)
    gATGCAGGTGCATGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAagcagc                                                                                                                                              <  7:105637/149‑1 (MQ=255)
         aGGTGCATGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCt                                                                                                                                           >  8:272593/1‑147 (MQ=255)
               aTGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCaatgt                                                                                                                                     >  1:263631/1‑143 (MQ=255)
               aTGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGAttt                                                                                                                                    >  6:154090/1‑148 (MQ=255)
                tGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTc                                                                                                                                   >  3:162949/1‑148 (MQ=255)
                  ccGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAAc                                                                                                                                >  3:231332/1‑149 (MQ=255)
                              aGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCTTTGCAGcccggt                                                                                                                                         <  1:203840/128‑4 (MQ=255)
                              aGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCTTTGCAGcccggt                                                                                                                                         >  2:203840/1‑125 (MQ=255)
                                         cAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAgg                                                                                                         <  2:290730/149‑1 (MQ=255)
                                          aGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGc                                                                                                        >  5:166998/1‑149 (MQ=255)
                                           ggCCAGCTTCCTGCCGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCg                                                                                                       <  1:126071/149‑1 (MQ=255)
                                                      tgctgcAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGcc                                                                                              >  8:258806/1‑147 (MQ=255)
                                                      tgctgcAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCa                                                                                            >  8:174203/1‑149 (MQ=255)
                                                        ctgcAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGc                                                                                          <  4:43693/149‑1 (MQ=255)
                                                        ctgcAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGc                                                                                          <  4:231332/149‑1 (MQ=255)
                                                           cAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCt                                                                                         <  7:174203/147‑1 (MQ=255)
                                                           cAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTg                                                                                        <  4:162949/148‑1 (MQ=255)
                                                            aggaggAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGTCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGa                                                                                      >  2:76784/1‑149 (MQ=255)
                                                            aggaggAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGa                                                                                      <  7:155575/149‑1 (MQ=255)
                                                                   gTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGc                                                                               >  7:79514/1‑149 (MQ=255)
                                                                   gTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGc                                                                               <  8:79514/149‑1 (MQ=255)
                                                                        aGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGc                                                                               >  5:268723/1‑144 (MQ=255)
                                                                        aGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGc                                                                               <  6:268723/144‑1 (MQ=255)
                                                                         gTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGAt                                                                                                                                      <  7:249680/88‑1 (MQ=255)
                                                                         gTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGAt                                                                                                                                      >  8:249680/1‑88 (MQ=255)
                                                                             gggCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGc                                                                      >  3:235704/1‑148 (MQ=255)
                                                                               gCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAAc                                                                                 >  7:239246/1‑135 (MQ=255)
                                                                               gCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAAc                                                                                 <  8:239246/135‑1 (MQ=255)
                                                                                   gggTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAATCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGc                                                               <  1:248319/149‑1 (MQ=255)
                                                                                           gTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGa                                                        <  6:166998/148‑1 (MQ=255)
                                                                                               gggCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACg                                                   <  5:154090/149‑1 (MQ=255)
                                                                                                ggcggcCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACgg                                                  <  7:258806/149‑1 (MQ=255)
                                                                                                  cggcCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGt                                                 >  4:265074/1‑148 (MQ=255)
                                                                                                      cAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCa                                            >  3:211014/1‑149 (MQ=255)
                                                                                                              gCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTTGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTgg                                    <  1:76784/149‑1 (MQ=255)
                                                                                                                tGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGa                                         >  3:227749/1‑142 (MQ=255)
                                                                                                                tGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGa                                         <  4:227749/142‑1 (MQ=255)
                                                                                                                       cTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACg                                                                       >  2:201732/1‑105 (MQ=255)
                                                                                                                       cTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACg                                                                       <  1:201732/105‑1 (MQ=255)
                                                                                                                               ggATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTgg                     >  3:114627/1‑147 (MQ=255)
                                                                                                                               ggATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTa                   <  4:235704/149‑1 (MQ=255)
                                                                                                                                    atctgagggCTGCTTTGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCa               >  7:74358/7‑148 (MQ=255)
                                                                                                                                       tgagggCTGCTTTGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGt                                >  8:192691/4‑128 (MQ=255)
                                                                                                                                       tgagggCTGCTTTGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGt                                <  7:192691/125‑1 (MQ=255)
                                                                                                                                       aCCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATAcc           >  4:21734/1‑149 (MQ=255)
                                                                                                                                        gagggCTGCTTTGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATAcc           >  7:272003/3‑148 (MQ=255)
                                                                                                                                        gagggCTGCTTTGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATAcc           >  7:267992/3‑148 (MQ=255)
                                                                                                                                        ccGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATAcc           <  7:272593/148‑1 (MQ=255)
                                                                                                                                              tGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATACCGATAGGc    >  8:73428/1‑149 (MQ=255)
                                                                                                                                                cAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATAc            >  2:118335/1‑139 (MQ=255)
                                                                                                                                                cAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATAc            <  1:118335/139‑1 (MQ=255)
                                                                                                                                                cAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATACCGATAGGCGa  >  1:216574/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGGGGATGCAGGTGCATGCCGAGGGCATCGAGCAGGCGGAACAGGCCAGCTTCCTGCTGCAGGAGGAGTGCCAGTTGGGGCAGGGGTACTGGTTCGGGCGGCCAGTGCCGGCTGAGGATCTTCGCTGGGATTGAGACCGGGCTGCAAAGCAGCCCTCAGATTTCAACGCTTGTTCAAACCCTTCGCCAGGCGGTCCCCGCCCAGCTGGATCAACGCCACTAACGCTACCAGCATGGCGATCACGGTCAGCATGATCTGGCTGTCGAAACGCTGGTAGCCATACCGATAGGCGA  >  NC_002947/271862‑272154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑