Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,964,530 C→T 8.3% A114T (GCC→ACC)  fatD ← ferric anguibactin transport system permease FatD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,964,5300CT 8.3% 135.4 / 2.3 48A114T (GCC→ACC) fatDferric anguibactin transport system permease FatD
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base T (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGCTTTGCAGCAGGTTGAAGCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTGCGCACGATCTG  >  NC_002947/2964384‑2964671
                                                                                                                                                  |                                                                                                                                             
cATGCTTTGCAGCAGGTTGAAGCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTggcggc                                                                                                                                               <  1:16144/147‑1 (MQ=255)
  tGCTTTGCAGCAGGTTGAAGCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCaaa                                                                                                                                            <  3:42646/148‑1 (MQ=255)
  tGCTTTGCAGCAGGTTGAAGCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCa                                                                                                                                              >  4:42646/1‑146 (MQ=255)
                    aGCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGcaca                                                                                                                                       >  4:162958/1‑135 (MQ=255)
                    aGCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGcaca                                                                                                                                       <  3:162958/135‑1 (MQ=255)
                     gCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGTAAAGCACACGGCCAGt                                                                                                                               <  8:72424/142‑1 (MQ=255)
                     gCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGTAAAGCACACGGCCAGt                                                                                                                               <  8:219285/142‑1 (MQ=255)
                     gCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGTAAAGCACACGGCCAGt                                                                                                                               >  7:72424/1‑142 (MQ=255)
                     gCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGTAAAGCACACGGCCAGt                                                                                                                               >  7:219285/1‑142 (MQ=255)
                              gTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATgcg                                                                                                                 >  8:111884/1‑147 (MQ=255)
                                 gAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCgcagca                                                                                                            <  6:115448/149‑1 (MQ=255)
                                                aCTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCgg                                                                                             <  1:97683/149‑1 (MQ=255)
                                                 cTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCggg                                                                                            >  1:50910/1‑149 (MQ=255)
                                                  tCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGgc                                                                                           >  1:122697/1‑149 (MQ=255)
                                                         gccgccGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGc                                                                                    >  1:184205/1‑149 (MQ=255)
                                                               gtgcATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGc                                                                               >  4:281478/4‑148 (MQ=255)
                                                                tACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGa                                                                             >  1:137667/1‑149 (MQ=255)
                                                                  cATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGAca                                                                           >  7:111364/1‑149 (MQ=255)
                                                                   aTCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGTGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGa                                                                             >  1:29108/1‑146 (MQ=255)
                                                                   aTCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGTGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGa                                                                             <  2:29108/146‑1 (MQ=255)
                                                                   aTCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGCcag                                                                          >  5:270687/1‑149 (MQ=255)
                                                                   aTCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGAcag                                                                          >  7:109296/1‑149 (MQ=255)
                                                                   aTCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGAcag                                                                          >  8:209226/1‑149 (MQ=255)
                                                                      aGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTg                                                                                                                              >  6:283575/1‑94 (MQ=255)
                                                                      aGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTg                                                                                                                              <  5:283575/94‑1 (MQ=255)
                                                                             tGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGccc                                                                <  2:50910/149‑1 (MQ=255)
                                                                               aCCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCa                                                               <  5:57482/148‑1 (MQ=255)
                                                                               aCCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAg                                                              <  6:270687/149‑1 (MQ=255)
                                                                                               cgTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGc                                              <  3:281478/149‑1 (MQ=255)
                                                                                                                 ggcggATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGt                            >  1:31798/1‑149 (MQ=255)
                                                                                                                       tGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAAc                       >  6:246104/1‑148 (MQ=255)
                                                                                                                        gAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCcgcg                                                                                              >  4:234069/1‑76 (MQ=255)
                                                                                                                        gAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCcgcg                                                                                              <  3:234069/76‑1 (MQ=255)
                                                                                                                          gggCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATgcgc                                                                                                                <  7:191973/56‑1 (MQ=255)
                                                                                                                          gggCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATgcgc                                                                                                                >  8:191973/1‑56 (MQ=255)
                                                                                                                             cGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCgg                              <  1:66656/135‑1 (MQ=255)
                                                                                                                             cGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCgg                              >  2:66656/1‑135 (MQ=255)
                                                                                                                              gACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACg                 <  3:236886/147‑1 (MQ=255)
                                                                                                                              gACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACg                 >  4:236886/1‑147 (MQ=255)
                                                                                                                              gACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGt               <  7:111884/149‑1 (MQ=255)
                                                                                                                              gACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGt               >  6:239309/1‑149 (MQ=255)
                                                                                                                                 aaaCAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCggtg                                     <  6:239344/124‑1 (MQ=255)
                                                                                                                                 aaaCAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCggtg                                     >  5:239344/1‑124 (MQ=255)
                                                                                                                                 aaaCAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTgc            <  7:209226/149‑1 (MQ=255)
                                                                                                                                 aaaCAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTgc            <  2:237788/149‑1 (MQ=255)
                                                                                                                                 aaaCAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTgc            >  3:59726/1‑149 (MQ=255)
                                                                                                                                    cagcagGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTGCGCa         <  2:184205/149‑1 (MQ=255)
                                                                                                                                     agcagGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTGCGCAc        <  5:239309/149‑1 (MQ=255)
                                                                                                                                           cTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTGCGCACGATctg  <  2:137667/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CATGCTTTGCAGCAGGTTGAAGCGGTAGGCGTAGAACTCGGCCAGCGCACTCAGCACGCCGCCGTACATCAGGCCGATGACCGGCACCAGCACCGCGTTGCGGGCGCGTATGCGGCGGATGAGGGCGACAAACAGCAGGCTGGCGGCAAAGCACACGGCCAGTGCGAACAGCATGCGCAGCAGTGCCCCGGCCGCGGGCGCCAGGCCCAGCGACAGCAGGATGCCCAGGCGCGCGGCATCGAGCCCGCCGGTGGTGCCGGGTTCAACGAAACGGTTGCGCACGATCTG  >  NC_002947/2964384‑2964671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑