Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,970,885 C→T 10.5% I408I (ATC→ATT)  PP_2599 → chemotaxis sensory transducer family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,970,8850CT10.5% 97.2 / 3.6 38I408I (ATC→ATT) PP_2599chemotaxis sensory transducer family protein
Reads supporting (aligned to +/- strand):  ref base C (17/17);  new base T (2/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GAGCGGGTGGGCGAGGCATTTCGGCGCTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCGTGTGG  >  NC_002947/2970748‑2971030
                                                                                                                                         |                                                                                                                                                 
gAGCGGGTGGGCGAGGCATTTCGGCGCTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAagag                                                                                                                                        >  5:259670/1‑149 (MQ=255)
      gTGGGCGAGGCATTTCGGCGCTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCta                                                                                                                                  >  5:196253/1‑149 (MQ=255)
              ggCATTTCGGCGCTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGCAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTg                                                                                                                          <  4:191374/149‑1 (MQ=255)
                 aTTTCGGCGCTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCAAAGAGCCCCTATACCGGTGGc                                                                                                                        >  6:60844/1‑148 (MQ=255)
                        ctcTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGa                                                                                                                <  6:196253/147‑1 (MQ=255)
                         gcTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGc                                                                                                                        >  5:262331/1‑140 (MQ=255)
                         gcTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGc                                                                                                                        <  6:262331/140‑1 (MQ=255)
                          cTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGc                                                                                                              >  8:15169/1‑149 (MQ=255)
                          cTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTCGCCACTGCGAGc                                                                                                              >  8:102368/1‑149 (MQ=255)
                             tGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCg                                                                                                             <  6:259670/147‑1 (MQ=255)
                                 gaggagCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGcc                                                                                                       >  7:66322/1‑149 (MQ=255)
                                   ggagCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCt                                                                                                      >  5:117368/1‑148 (MQ=255)
                                      gCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGc                                                                                                  >  8:159798/1‑149 (MQ=255)
                                          tCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTg                                                                                                <  8:183398/147‑1 (MQ=255)
                                          tCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGgc                                                                                              >  3:113160/1‑149 (MQ=255)
                                                             ccATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAg                                                                           <  6:117368/149‑1 (MQ=255)
                                                             ccATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAg                                                                           >  3:213190/1‑149 (MQ=255)
                                                               aTCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGt                                                                                                                           >  4:279612/1‑99 (MQ=255)
                                                               aTCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGt                                                                                                                           <  3:279612/99‑1 (MQ=255)
                                                                tCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAgg                                                                          >  8:68278/1‑147 (MQ=255)
                                                                  gAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGt                                                                      <  8:66322/149‑1 (MQ=255)
                                                                    aaCCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTgg                                                                    <  7:159798/149‑1 (MQ=255)
                                                                           cAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCa                                                                            <  6:150040/134‑1 (MQ=255)
                                                                             gTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGa                                                             >  6:173789/1‑147 (MQ=255)
                                                                             gTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACa                                                           <  5:173789/149‑1 (MQ=255)
                                                                                  tCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGt                                                      <  4:213190/149‑1 (MQ=255)
                                                                                                           cGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCg                             <  4:113160/149‑1 (MQ=255)
                                                                                                             ccATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAg                           <  3:162213/149‑1 (MQ=255)
                                                                                                                     aaGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCg                   <  7:102368/149‑1 (MQ=255)
                                                                                                                              gCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCATCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAg            <  5:60844/147‑1 (MQ=255)
                                                                                                                                    gCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGc                                                                         <  4:98312/80‑1 (MQ=255)
                                                                                                                                    gCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGc                                                                         >  3:98312/1‑80 (MQ=255)
                                                                                                                                    gCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCgtgt    >  1:162008/1‑149 (MQ=255)
                                                                                                                                      cATTGATGCCAAGAGCTCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCg       <  7:150978/144‑1 (MQ=255)
                                                                                                                                      cATTGATGCCAAGAGCTCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCg       >  8:150978/1‑144 (MQ=255)
                                                                                                                                      cATTGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCg       >  4:48548/1‑144 (MQ=255)
                                                                                                                                      cATTGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCg       <  3:48548/144‑1 (MQ=255)
                                                                                                                                       aTCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCGTGTgg  >  5:204190/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                 
GAGCGGGTGGGCGAGGCATTTCGGCGCTTTGTCGAGGAGCTGTCCGGTGTGGCTGCAGTGGCCATCGAAACCCGCCAGTTGGTCGAAGCCCAGCAACGCCTGCTGGACGCCATGATCAAGCTGCTGGCAGACGCCATCGATGCCAAGAGCCCCTATACCGGTGGCCACTGCGAGCGGGTGCCTCAGCTGGCGCAGATGCTGCTGGATCAGGCAGTGGCAGCCGACAGCGGTCCGTTCGCCAGCTTCAGCATGACCGAGGCAGAGCGGTACGAGTTCCGTGTGG  >  NC_002947/2970748‑2971030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑