Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,977,238 A→G 8.0% Y373H (TAC→CAC)  PP_2604 ← major facilitator family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,977,2380AG 8.0% 140.4 / 3.1 50Y373H (TAC→CAC) PP_2604major facilitator family transporter
Reads supporting (aligned to +/- strand):  ref base A (26/20);  new base G (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

ATGGCCGGGATCATGGTAAACAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTGCAGGCAAAGATCAGCATGGACAC  >  NC_002947/2977090‑2977385
                                                                                                                                                    |                                                                                                                                                   
aTGGCCGGGATCATGGTAAACAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGTGTGTGTa                                                                                                                                                     >  8:281718/1‑149 (MQ=255)
 tGGCCGGGATCATGGTAAACAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAg                                                                                                                                                    >  6:258620/1‑149 (MQ=255)
 tGGCCGGGATCATGGTAAACAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAg                                                                                                                                                    >  2:195883/1‑149 (MQ=255)
   gCCGGGATCATGGTAAACAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTc                                                                                                                                                  <  1:195883/149‑1 (MQ=255)
             tGGTAAACAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAgg                                                                                                                                        >  7:41480/1‑149 (MQ=255)
                  aaCAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTc                                                                                                                                                  >  4:287009/1‑134 (MQ=255)
                  aaCAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTc                                                                                                                                                  <  3:287009/134‑1 (MQ=255)
                  aaCAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTcc                                                                                                                                    <  7:281718/148‑1 (MQ=255)
                  aaCAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCa                                                                                                                                   <  8:41480/149‑1 (MQ=255)
                         cGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTc                                                                                                                            <  1:191315/149‑1 (MQ=255)
                            cATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTGGTCCATCACCAGGCTCCAGATATTCGcc                                                                                                                         >  6:186961/1‑149 (MQ=255)
                            cATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTGGTCCATCACCAGGCTCCAGATATTCGcc                                                                                                                         >  6:182640/1‑149 (MQ=255)
                             aTAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCg                                                                                                                        <  1:32433/149‑1 (MQ=255)
                                gttgttgttGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTc                                                                                                                      >  1:261/1‑148 (MQ=255)
                                      gttgGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCt                                                                                                               >  7:129823/1‑149 (MQ=255)
                                       ttgGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCt                                                                                                               <  1:270862/148‑1 (MQ=255)
                                       ttgGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTg                                                                                                              >  7:179424/1‑149 (MQ=255)
                                       ttgGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGCAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCt                                                                                                               <  7:186498/148‑1 (MQ=255)
                                       ttgGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAACACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTg                                                                                                              >  2:266415/1‑149 (MQ=255)
                                          gTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTg                                                                                                           >  4:145600/1‑149 (MQ=255)
                                                gTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGcc                                                                                                     >  8:80295/1‑149 (MQ=255)
                                                gTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGcc                                                                                                     >  3:108513/1‑149 (MQ=255)
                                                   aGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTAc                                                                                                  <  2:103857/149‑1 (MQ=255)
                                                        gCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGa                                                                                             <  4:108513/149‑1 (MQ=255)
                                                                       cGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTGGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGgcc                                                                                  <  5:182640/145‑1 (MQ=255)
                                                                       cGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTGGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGgcc                                                                                  <  5:186961/145‑1 (MQ=255)
                                                                           aTGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACcgc                                                                          >  2:132926/1‑149 (MQ=255)
                                                                           aTGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACcg                                                                           <  7:80295/148‑1 (MQ=255)
                                                                              aaCATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGGTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGGCTACCGCGATCGAGATGGCCGCCACCGCGAc                                                                       <  8:179424/149‑1 (MQ=255)
                                                                               aCATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGAcc                                                                      >  4:1014/1‑149 (MQ=255)
                                                                                 aTCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCca                                                                    >  7:289543/1‑149 (MQ=255)
                                                                                    ccGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCcaca                                                                  <  3:145600/148‑1 (MQ=255)
                                                                                     cGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGt                                                                >  2:305982/1‑149 (MQ=255)
                                                                                           ttGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTg                                                          <  8:129823/149‑1 (MQ=255)
                                                                                                gCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGtt                                                     >  6:29964/1‑149 (MQ=255)
                                                                                                  acacaTGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTgcgc                                                                                                        <  2:20253/96‑1 (MQ=255)
                                                                                                  acacaTGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTgcgc                                                                                                        >  1:20253/1‑96 (MQ=255)
                                                                                                   cacaTGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGgc                                                  <  5:258620/149‑1 (MQ=255)
                                                                                                          tgcCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGgcagca                                              >  6:148519/1‑146 (MQ=255)
                                                                                                            ccgaagccgaaTACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAg                                           <  3:1014/147‑1 (MQ=255)
                                                                                                               aagccgaaTACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGAc                                      >  2:20911/1‑149 (MQ=255)
                                                                                                                agccgaaTACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACg                                     >  6:222816/1‑149 (MQ=255)
                                                                                                                    gaaTACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCt                                   >  3:213388/1‑147 (MQ=255)
                                                                                                                            tGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTg                         <  1:20911/149‑1 (MQ=255)
                                                                                                                                 aCCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTGCAg                      <  5:29964/147‑1 (MQ=255)
                                                                                                                                  ccAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTGCAGGc                    >  6:110186/1‑148 (MQ=255)
                                                                                                                                        gCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATg                            <  2:33372/134‑1 (MQ=255)
                                                                                                                                        gCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATg                            >  1:33372/1‑134 (MQ=255)
                                                                                                                                        gCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACAGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTGCAGGCAAAGAt              >  5:213141/1‑148 (MQ=255)
                                                                                                                                            gggTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTGCAGGCAAAGATCAGCa         >  8:163232/1‑149 (MQ=255)
                                                                                                                                                   tAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTGCAGGCAAAGATCAGCATGGacac  >  3:13891/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGGCCGGGATCATGGTAAACAGCACGGCATAGTTGTTGTTGGTAGCGGTCAGCACGCTGCCGACAATCTGCGTCATGAACATCCCGCCAATTGCCGCACACATGCTGCCGAAGCCGAATACGGTGCTCACCAGATGCTTGGGTGTGTAGTCCATCACCAGGCTCCAGATATTCGCCGTCCACGCCTGGTGCGCGCCTACCGCGATCGAGATGGCCGCCACCGCGACCCACAGTCCACTGGCGTTGGCAGCAAAGATGACGCTGAGCATGGTGCAGGCAAAGATCAGCATGGACAC  >  NC_002947/2977090‑2977385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑