| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,139,185 | C→T | 10.8% | Q442* (CAG→TAG) | PP_2754 → | OprD family outer membrane porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,139,185 | 0 | C | T | 10.8% | 92.8 / 2.9 | 37 | Q442* (CAG→TAG) | PP_2754 | OprD family outer membrane porin |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (2/2); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01 | |||||||||||
CCACTGGGAGCGCAACCTGTGGCTGAAGTACGTGCTGCAGAGTGGGCCGGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAGTGTCCGCCCGGCAACAGCCAAT > NC_002947/3139040‑3139327 | ccACTGGGAGCGCAACCTGTGGCTGAAGTACGTGCTGCAGAGTGGGCCTGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGt < 2:73992/149‑1 (MQ=255) ccTGTGGCTGAAGTACGTGCTTCAGAGTGGGCCGGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCa < 3:16377/149‑1 (MQ=255) ccTGTGGCTGAAGTACGTGCTGCAGAGTGGGCCGGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGACGCCGAGTCCAATCTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGatat > 1:129035/1‑147 (MQ=255) gCTGAAGTACGTGCTGCAGAGTGGGCCGGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCct > 7:91886/1‑147 (MQ=255) gCTGAAGTACGTGCTGCAGAGTGGGCCGGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCct < 8:91886/147‑1 (MQ=255) aaGTACGTGCTGCAGAGTGGGCCGGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGa < 3:97062/147‑1 (MQ=255) gccggccAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTcgc > 7:85588/1‑147 (MQ=255) gccggccAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCga < 7:65821/149‑1 (MQ=255) ggccAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCAGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCgaga > 6:155017/1‑148 (MQ=255) aaGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATc < 4:143025/111‑1 (MQ=255) aaGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATc > 3:143025/1‑111 (MQ=255) ggACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCga < 8:105532/140‑1 (MQ=255) ggACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCga > 7:105532/1‑140 (MQ=255) gTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCTAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAacac > 4:239501/1‑149 (MQ=255) gTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCTAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAacac > 6:109862/1‑149 (MQ=255) aaCCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGAt > 8:213373/1‑149 (MQ=255) ccTGATGCAGGCCACCCACCGCGTCGGTGGCGATCTCGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGAt < 8:248467/147‑1 (MQ=255) acccaccGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCaaa > 4:41290/1‑148 (MQ=255) acccaccGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCaaa > 2:172678/1‑148 (MQ=255) caccGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCTAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACa < 3:239501/149‑1 (MQ=255) caccGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCTAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACa < 5:109862/149‑1 (MQ=255) cgagTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGc < 2:129035/146‑1 (MQ=255) gCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGt < 7:213373/149‑1 (MQ=255) ccGAGTCCAATGTCGACGAACTCCGCCAGATCGTCCAGTAACCGCTCGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCCAGATACCGCCATGCAACACCGAAGGCGGCCTGCCAAAAACAGCCGAGCAGCGGTCGGCAGGTACt > 5:166867/1‑148 (MQ=255) gTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGc < 8:85588/149‑1 (MQ=255) tCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGc < 7:272219/148‑1 (MQ=255) tCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGc > 8:272219/1‑148 (MQ=255) tCCAATCTCGACGAACTGCGCCTGATAGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCTAGGAGCGGTCGGCAGGTTCTGAGGc > 5:241846/1‑148 (MQ=255) aaTGTGGAGGAACTGCGCCTGATCGTCCAGTAGCCGCTTGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCTTGCAACACCGATGGCGGCCTGGCAAAAACAGGGGAGGAGCGGTCGGCAGGTTCTGAGGCAAgt < 6:166867/149‑1 (MQ=255) gacgaACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAGTGTCCGc > 4:276348/1‑149 (MQ=255) aCTGCGGCTGATCGTCCAGTACCCTCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAGTGTCCGCCCGGc < 6:241846/149‑1 (MQ=255) cTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAg > 7:267327/1‑125 (MQ=255) cTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAg < 8:267327/125‑1 (MQ=255) tGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAgtg < 7:244002/131‑1 (MQ=255) tGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGAGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAgtg > 8:244002/1‑131 (MQ=255) aTCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAGTGTCCGCCCGGCAACAGCCAAt > 8:6617/1‑149 (MQ=255) aTCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAGTGTCCGCCCGGCAACAGCCAAt > 4:137876/1‑149 (MQ=255) | CCACTGGGAGCGCAACCTGTGGCTGAAGTACGTGCTGCAGAGTGGGCCGGCCAAGGACCTCTCGTTCAACCTGATGCAGGCCACCCACCGCGTCGGTGGCGATCACGTCGCCGAGTCCAATGTCGACGAACTGCGCCTGATCGTCCAGTACCCGCTGGATATCAGCCTCTGAGCACCAGCTCACGGTCTCGCGAGATACCGCCATGCAACACCGATGGCGGCCTGGCAAAAACAGGCGAGGAGCGGTCGGCAGGTTCTGAGGCAAGTGTCCGCCCGGCAACAGCCAAT > NC_002947/3139040‑3139327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |