Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,164,142 T→C 9.1% V311A (GTG→GCG)  PP_2778 → 3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,164,1420TC 9.1% 116.6 / 3.0 44V311A (GTG→GCG) PP_27783‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base C (2/2);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAATCTGCCGTAGGGCATTCGTTGGC  >  NC_002947/3163998‑3164280
                                                                                                                                                |                                                                                                                                          
gtcAGGCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGCGGAc                                                                                                                                        >  5:263015/1‑149 (MQ=255)
gtcAGGCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGCGGAc                                                                                                                                        >  5:267703/1‑149 (MQ=255)
   aGGCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCa                                                                                                                                      <  5:18254/148‑1 (MQ=255)
     gCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                                                                   <  1:244376/149‑1 (MQ=255)
     gCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                                                                   <  4:191976/149‑1 (MQ=255)
     gCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCAtgt                                                                                                                                   >  8:214939/1‑149 (MQ=255)
            cGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGtt                                                                                                                            >  5:16511/1‑149 (MQ=255)
              cTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCa                                                                                                                          <  2:79983/149‑1 (MQ=255)
                  gCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGt                                                                                                                      <  5:32841/149‑1 (MQ=255)
                       gTGCTGGGTTACGCGAATCTGTGCGACGCAGAGAAAATCAACTCCAGCAGCCGCGACGGCATGAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTc                                                                                                                 <  5:78656/149‑1 (MQ=255)
                            gggTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGaca                                                                                                             >  2:161432/1‑148 (MQ=255)
                              gTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCAGATGCAGAAATGACAAGTGCGGCTGTGGAACATGTGAATGTTCACGGTACGTCGACACAg                                                                                                          >  1:43053/1‑149 (MQ=255)
                                 aCGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGc                                                                                                        <  6:16511/148‑1 (MQ=255)
                                       aTCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGCGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGAc                                                                                                 <  6:263015/149‑1 (MQ=255)
                                       aTCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGCGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGAc                                                                                                 <  6:267703/149‑1 (MQ=255)
                                            tgCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGAcc                                                                                                <  7:176314/145‑1 (MQ=255)
                                             gCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGc                                                                                           >  6:183949/1‑149 (MQ=255)
                                                  gCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGc                                                                                      <  1:247280/149‑1 (MQ=255)
                                                    agagAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGccc                                                                                    >  5:151998/1‑149 (MQ=255)
                                                     gagaAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCt                                                                                   >  5:118418/1‑149 (MQ=255)
                                                       gaAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTgg                                                                                 >  3:33365/1‑149 (MQ=255)
                                                        aaaaTCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTggg                                                                                >  8:234218/1‑149 (MQ=255)
                                                                cTCCAGCAGCCGCGACGGAAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGCCCAGTGCGGCTGTGGACCAGGTGAATGTTCACGGTACGTCGCCACAggg                                                                                                        >  3:165725/1‑116 (MQ=255)
                                                                  ccAGCACCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGACCGATGCCGAACTGACCACAGCGGATGTGGACCAATTGAATCTTCACGGTAAGTCGACACAGGCCAATGACCGTTGCGAGGCCCTCGGACTGCCGCAg                                                                      >  4:254410/1‑149 (MQ=255)
                                                                   cagcagCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAgg                                                                     <  6:151998/149‑1 (MQ=255)
                                                                   cagcagCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAg                                                                      <  7:234218/148‑1 (MQ=255)
                                                                         ccGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGCGGCCCTGGGCCTGGCGCAGGTTTTCg                                                               <  1:161432/149‑1 (MQ=255)
                                                                          cgcgACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCgg                                                              <  5:183949/149‑1 (MQ=255)
                                                                               cGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGaaa                                                         <  3:294592/149‑1 (MQ=255)
                                                                                   agcaAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCt                                                     >  5:56336/1‑149 (MQ=255)
                                                                                             ccgccgTCATGCAGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGAt                                            >  3:97305/1‑148 (MQ=255)
                                                                                                ccTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACg                                        <  4:33365/149‑1 (MQ=255)
                                                                                                       gACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGCCCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCg                                 >  8:265128/1‑149 (MQ=255)
                                                                                                        caggcggcCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGc                                <  4:97305/147‑1 (MQ=255)
                                                                                                                ccTGGCCGCTGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAAt                        <  2:245896/149‑1 (MQ=255)
                                                                                                                ccTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGGACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGTGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAAt                        <  4:53898/149‑1 (MQ=255)
                                                                                                                      cGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCgg                                                              <  1:188895/105‑1 (MQ=255)
                                                                                                                      cGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCgg                                                              >  2:188895/1‑105 (MQ=255)
                                                                                                                           ccGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAATCTGCCGTAgg              <  3:254410/148‑1 (MQ=255)
                                                                                                                            cGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTg                                                                            <  4:221118/85‑1 (MQ=255)
                                                                                                                            cGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTg                                                                            >  3:221118/1‑85 (MQ=255)
                                                                                                                             gAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAATCTGCCGTAGGGCa           >  5:105579/1‑149 (MQ=255)
                                                                                                                                      aGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACATGCCAATGACCGTTGCGAGGCCCTGTTCCTGGCTCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAATCTGCCGTAGGGCATTCGTTGGc  <  2:43053/149‑1 (MQ=255)
                                                                                                                                      aGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAATCTGCCGTAGGGCATTCGTTGGc  <  7:265128/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                          
GTCAGGCCTGTTCGCTGGGCCGGGTGCTGGGTTACGCGAATCTGTGCGAGGCAGAGAAAATCACCTCCAGCAGCCGCGACGGCAGCAAGTATGCCGCCTGCATGACGGCGGCCCTGGCCGATGCCGAACTGACCAGTGCGGCTGTGGACCATGTGAATGTTCACGGTACGTCGACACAGGCCAATGACCGTTGCGAGGCCCTGGGCCTGGCGCAGGTTTTCGGCGAAACCCTGGAGCAGATGACGTTCACCGCCAACAAATCTGCCGTAGGGCATTCGTTGGC  >  NC_002947/3163998‑3164280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑