Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,173,790 G→C 9.4% intergenic (+11/‑40) PP_2785 → / → PP_2786 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,173,7900GC 9.4% 111.5 / 2.5 41intergenic (+11/‑40)PP_2785/PP_2786hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/19);  new base C (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

TCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCCCGG  >  NC_002947/3173649‑3173937
                                                                                                                                             |                                                                                                                                                   
tCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGtgtg                                                                                                                                              >  2:191219/1‑149 (MQ=255)
 cACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACGCCGGTGCGGTGTGc                                                                                                                                             >  7:26313/1‑149 (MQ=255)
    cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACGCCGGTGCGGTGTGCGaa                                                                                                                                          <  8:26313/149‑1 (MQ=255)
    cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggt                                                                                                                                            <  8:179895/147‑3 (MQ=255)
    cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCAAACCGCACCGGtgtggt                                                                                                                                            >  7:179895/1‑145 (MQ=255)
    cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTACGGCAAGCCCGACCGCCAGTTCGGTGCGTCCGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGaa                                                                                                                                          >  3:182035/1‑149 (MQ=255)
     tACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAAc                                                                                                                                         >  8:201095/1‑149 (MQ=255)
      aCAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAAc                                                                                                                                         >  3:251208/1‑148 (MQ=255)
       cAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCa                                                                                                                                       <  1:54424/149‑1 (MQ=255)
       cAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCa                                                                                                                                       <  3:123685/149‑1 (MQ=255)
         aaCCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACt                                                                                                                                     <  5:183313/149‑1 (MQ=255)
             ggTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCaaa                                                                                                                                 <  1:191219/149‑1 (MQ=255)
             ggTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCaaa                                                                                                                                 <  7:205762/149‑1 (MQ=255)
                      gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGACAGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACAACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                          >  7:81145/1‑147 (MQ=255)
                       cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                          >  1:184529/1‑126 (MQ=255)
                       cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                          <  2:184529/130‑5 (MQ=255)
                            gTTGGGGCTCGAAGTCGATGTGTTCTTCTGCAAGACCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCAGGTTATCTGTTCTGACCCCACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAAt                                                                                                                  >  7:285252/1‑149 (MQ=255)
                                gggCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGcc                                                                                                              <  7:118480/149‑1 (MQ=255)
                                       aaGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGc                                                                                                       <  7:65950/149‑1 (MQ=255)
                                             aTGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCt                                                                                                 >  4:265849/1‑149 (MQ=255)
                                                  ttcttcGGCAAGCCCGACCGCGAGTTCGGTGCGTACGACAACCGCGATCAGGTGAGCTTGTCCGCCGGTTATCTGTTCTGACCACACCTGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCaa                                                                                             <  8:81145/148‑1 (MQ=255)
                                                    cttgGGCAAGCCCGAGCGCCAGTTCGGTGCGTACGTCAACCGCGATGAGGTGCGCTTGTCCGCCGGTAATCTGTTCTGACCAGACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGTAATCGCCTTGTTGCCCATCTTCAAGa                                                                                           <  4:251208/148‑1 (MQ=255)
                                                    cttcGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGAc                                                                                          >  7:203654/1‑149 (MQ=255)
                                                       cGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                       <  6:31140/117‑1 (MQ=255)
                                                       cGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                       >  5:31140/1‑117 (MQ=255)
                                                                      ccAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCAct                                                                        >  5:29732/1‑149 (MQ=255)
                                                                              gTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTTTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGa                                                                <  7:201095/149‑1 (MQ=255)
                                                                                 cGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGTTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAgc                                                              <  7:280731/148‑1 (MQ=255)
                                                                                   tACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGcgcc                                                           >  6:34181/1‑149 (MQ=255)
                                                                                      gACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCt                                                        >  4:230343/1‑149 (MQ=255)
                                                                                                      tGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCt                                         >  3:59218/1‑148 (MQ=255)
                                                                                                         gcTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGaaa                                     <  8:203654/149‑1 (MQ=255)
                                                                                                               ccgccgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAAcacgc                                <  3:230343/148‑1 (MQ=255)
                                                                                                                  ccgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGAGCCGCTGCCGCCATGGCAGCTGAAACAAGCACgg                            >  1:72396/1‑149 (MQ=255)
                                                                                                                   cgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGc                           <  6:29732/149‑1 (MQ=255)
                                                                                                                    ggTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGcc                          >  1:157673/1‑149 (MQ=255)
                                                                                                                            gTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCACGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAcat                  >  8:58894/1‑149 (MQ=255)
                                                                                                                             ttCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAca                   <  4:59218/147‑1 (MQ=255)
                                                                                                                                  aCCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAATTCGCCATGTTGCCCATGTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTc             <  4:182035/148‑1 (MQ=255)
                                                                                                                                    cacaCCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAac                                    >  6:64920/1‑123 (MQ=255)
                                                                                                                                    cacaCCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAac                                    <  5:64920/123‑1 (MQ=255)
                                                                                                                                             gcggtgTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCCCgg  >  3:180138/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCCCGG  >  NC_002947/3173649‑3173937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑