| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,174,739 | G→A | 10.8% | A14T (GCA→ACA) | PP_2787 → | transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,174,739 | 0 | G | A | 10.8% | 96.6 / 3.0 | 37 | A14T (GCA→ACA) | PP_2787 | transporter |
| Reads supporting (aligned to +/- strand): ref base G (15/18); new base A (2/2); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CTGTTCACGCAGACCGCGATCAAGCGCGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCATTGATCTGCAGCGCGAGTTCACCG > NC_002947/3174592‑3174880 | cTGTTCACGCAGACCGCGATCAAGCGCGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGc < 6:172373/149‑1 (MQ=255) gTTCACGCAGACCGCGATCAAGCGCGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCaa > 6:237710/1‑149 (MQ=255) gCAGACCGCGATCAAGCGCGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTgtgt < 5:237710/149‑1 (MQ=255) gCAGACCGCGATCAAGCGCGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGACAATTgtgt > 4:192230/1‑149 (MQ=255) gACCGCGATCAAGCGCGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCg < 3:137563/149‑1 (MQ=255) gcgcGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCATCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGa > 3:222343/1‑149 (MQ=255) gCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACAcc < 7:279403/148‑1 (MQ=255) gCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACAcc < 6:266684/148‑1 (MQ=255) gCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGACAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACAcc > 3:291353/1‑148 (MQ=255) gAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCgg < 4:19538/149‑1 (MQ=255) aaTACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTgtgt < 4:31971/105‑1 (MQ=255) aaTACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTgtgt > 3:31971/1‑105 (MQ=255) gCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCa < 2:154208/121‑1 (MQ=255) gCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCa > 1:154208/1‑121 (MQ=255) cccGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCg < 6:26020/146‑1 (MQ=255) cccGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCg < 5:22108/146‑1 (MQ=255) gcgcCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCTGCGCGCTGATGTCTGacac < 6:18865/143‑1 (MQ=255) gcgcCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGacac > 5:18865/1‑143 (MQ=255) tctcGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGaca > 4:254976/1‑135 (MQ=255) tctcGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGaca < 3:254976/135‑1 (MQ=255) ctcGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGc > 2:209922/1‑149 (MQ=255) aTTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTc > 8:168612/1‑147 (MQ=255) tCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGaca > 2:35358/1‑149 (MQ=255) gAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTTGCGATCACCGCCTACTTCACGTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGacac < 4:222343/146‑1 (MQ=255) gAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGaca < 3:240975/145‑1 (MQ=255) gAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGACAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGaca < 3:192230/145‑1 (MQ=255) tcattcatGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGt > 5:78524/1‑124 (MQ=255) tcattcatGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGt < 6:78524/124‑1 (MQ=255) ggAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGa > 5:243458/1‑149 (MQ=255) tGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGAAGATCATTGAt > 5:305160/1‑149 (MQ=255) gAACGTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCATTGATc > 5:37052/1‑149 (MQ=255) aaCTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCATTGAt < 2:62700/147‑1 (MQ=255) aCTTCGTCGAGCGCTATGCGCGGACAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCATTGATCTg < 4:291353/149‑1 (MQ=255) gaccgcTATGCGCGGGCAATTGTGTTCGTCTTGGTGGCGATAACCGCCTACTTCACCTTCACCCTCGGCGCGATGATGTCGGACACAAATCCGTATCTGCTCAAGGACAATCACCCGTCGCTCAAGACGAACATTCAACTGCAgcga > 4:220662/4‑146 (MQ=255) tATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCAt < 2:137537/128‑1 (MQ=255) tATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCAt > 1:137537/1‑128 (MQ=255) tGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCATTGATCTGCAGCGCGAGTTCACCg < 6:243458/149‑1 (MQ=255) | CTGTTCACGCAGACCGCGATCAAGCGCGGGCTGAAAGCCGGCGACTTGCCGGAATACGCCGTGTCTGCCCGCTAAGCGCCGCTCTCGATTCATCTGGAACCCTCATTCATGGAAAGATACCTGAACTTCGTCGAGCGCTATGCGCGGGCAATTGTGTTCGTGTTGGTGGCGATCACCGCCTACTTCACCTACACCCTCGGCGCGCTGATGTCGGACACCAATCCGTATCTGCTCAAGGACACTCACCCGGCGCGCAAGACGATCATTGATCTGCAGCGCGAGTTCACCG > NC_002947/3174592‑3174880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |