Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,190,795 G→A 10.2% Q402* (CAG→TAG)  PP_2800 ← diaminobutyrate‑2‑oxoglutarate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,190,7950GA10.2% 104.3 / 2.5 39Q402* (CAG→TAG) PP_2800diaminobutyrate‑2‑oxoglutarate transaminase
Reads supporting (aligned to +/- strand):  ref base G (19/16);  new base A (2/2);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTCATGTTCCTGTCGCAGGGCGAGGGTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCGGCCGAAATCGATG  >  NC_002947/3190651‑3190943
                                                                                                                                                |                                                                                                                                                    
ggCTCATGTTCCTGTCGCAGGGCGAGGGTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTc                                                                                                                                                   <  7:239228/148‑1 (MQ=255)
   tCATGTTCCTGTCGCAGGGCGAGGGTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGc                                                                                                                                               >  8:76521/1‑149 (MQ=255)
       gTTCCTGTCGCAGGGCGAGGGTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTggt                                                                                                                                           >  5:16459/1‑149 (MQ=255)
       gTTCCTGTCGCAGGGCGAGGCTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTggt                                                                                                                                           >  3:230819/1‑149 (MQ=255)
              tCGCAGGGCGAGGGTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGtcggtcgg                                                                                                                                    >  2:103807/1‑149 (MQ=255)
                      cGAGGGTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCa                                                                                                                            <  6:16459/149‑1 (MQ=255)
                               cccGCAGGTGAGCAGGTGGCGTGCGCCCTGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTGAGGCCCTGTTCGAGCTGGTCGGGCGGGGTGTTCTCCggcggc                                                                                                                    <  2:62419/148‑1 (MQ=255)
                               cccGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCa                                                                                                                   >  1:100824/1‑149 (MQ=255)
                                ccGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCAt                                                                                                                  >  5:211803/1‑149 (MQ=255)
                                ccGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCAt                                                                                                                  >  2:131991/1‑149 (MQ=255)
                                      gTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCtt                                                                                                            >  4:137095/1‑149 (MQ=255)
                                       tGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTtg                                                                                                           <  6:211803/149‑1 (MQ=255)
                                         aGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTtgat                                                                                                         <  1:103807/149‑1 (MQ=255)
                                                        cccAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCgg                                                                                          <  1:131991/149‑1 (MQ=255)
                                                                 cGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCg                                                                                 >  6:250851/1‑149 (MQ=255)
                                                                  gCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACcggc                                                                                                                       <  2:228164/110‑1 (MQ=255)
                                                                  gCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACcggc                                                                                                                       >  1:228164/1‑110 (MQ=255)
                                                                  gCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCgg                                                                                >  7:82463/1‑149 (MQ=255)
                                                                   cTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTATTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGt                                                                               >  1:70537/1‑149 (MQ=255)
                                                                        ggTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGAcc                                                                          <  6:176769/149‑1 (MQ=255)
                                                                                 cTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGccc                                                                 >  7:203232/1‑149 (MQ=255)
                                                                                     aCCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGCCCGCAGCGGTCGACCAGCACGCCCGCtt                                                             <  1:175869/149‑1 (MQ=255)
                                                                                                 cTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTATTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACgcgc                                                  <  2:70537/148‑1 (MQ=255)
                                                                                                    gCGAAGCGCTGCCGCGACCGCCTCGCCGCAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCgctgct                                              >  8:229229/1‑149 (MQ=255)
                                                                                                      gAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTgg                                            <  5:250851/149‑1 (MQ=255)
                                                                                                               ccGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTg                                   <  7:76521/149‑1 (MQ=255)
                                                                                                                 gcgACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGc                                 >  3:34442/1‑149 (MQ=255)
                                                                                                                  cgACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCa                                                          <  6:218536/123‑1 (MQ=255)
                                                                                                                  cgACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCa                                                          >  5:218536/1‑123 (MQ=255)
                                                                                                                   gACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGcc                                >  4:39358/1‑148 (MQ=255)
                                                                                                                        ccTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGc                           >  2:72666/1‑148 (MQ=255)
                                                                                                                         cTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAAcgcg                                                   <  6:59953/123‑1 (MQ=255)
                                                                                                                         cTCGCCGAAGGCGTTCAGCCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAAcgcg                                                   >  5:59953/1‑123 (MQ=255)
                                                                                                                                    cGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCgg              >  1:297159/1‑149 (MQ=255)
                                                                                                                                    cGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCgg              <  2:100824/149‑1 (MQ=255)
                                                                                                                                          ggCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCGGCCGaaa        <  4:34442/149‑1 (MQ=255)
                                                                                                                                          ggCCCTATTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCGGc             <  4:121758/144‑1 (MQ=255)
                                                                                                                                          ggCCCTATTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCGGc             >  3:121758/1‑144 (MQ=255)
                                                                                                                                           gCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCGGCCGAAAt       <  3:137095/149‑1 (MQ=255)
                                                                                                                                                gTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCGGACGAAATCGATg  >  1:157244/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGCTCATGTTCCTGTCGCAGGGCGAGGGTTGCCCGCAGGTGAGCAGGTGGCGTGCGCCCAGTGTCCGCTCCGGGTGCACGCCTGGACCCTGCAGTTACTGGCGAAGCGCTGCCGCGACCGCCTCGCCGAAGGCGTTCAGGCCCTGTTCGAGCTGGTCGGTCGGGGTGTTCACCGGCGGCATCAGCTTGATGATTTCGCCATTCGGGCCGCAGCGGTCGACCAGCACGCCCGCTTGCAGAACGCGCTGCTGGGCATCCTTGGCCAAGGCCACGTTGCCGCGGCCGAAATCGATG  >  NC_002947/3190651‑3190943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑