| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,210,906 | G→A | 11.7% | intergenic (‑181/‑58) | PP_2814 ← / → PP_2815 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,210,906 | 0 | G | A | 11.7% | 84.0 / 3.4 | 34 | intergenic (‑181/‑58) | PP_2814/PP_2815 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (16/14); new base A (2/2); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
GCAGGCGGGTTGACGTTTGATCAACGGGCATCAGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGC > NC_002947/3210758‑3211051 | gcAGGCGGGTTGACGTTTGATCAACGGGCATCAGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTg > 4:114388/1‑149 (MQ=255) gggTTGACGTTTGATCAACGGGCATCAGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGcc > 7:210154/1‑147 (MQ=255) ggTTGACGTTTGATCAACGGTCATCAGTAAACAGGCCTGCTTGACACTGCGCTAAACCCAAAAAAGTGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGAAGTGAGTTCATTCTAGGTTTCCACTGGCCCTGCGCCAAg < 8:210154/149‑1 (MQ=255) gTTTGATCAACGGGCATCAGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGaa > 5:111392/1‑149 (MQ=255) tCAGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTACGCCAAGGTACGAACTGCACCATT‑TTCCAg > 2:192787/1‑149 (MQ=255) tCAGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTACGCCAAGGTACGAACTGCACCATT‑TTCCAg > 2:197109/1‑149 (MQ=255) aGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGcc > 1:122728/1‑149 (MQ=255) aGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGc < 2:244701/148‑1 (MQ=255) ccTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAg > 5:104651/1‑149 (MQ=255) gCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGAtt < 6:111392/147‑1 (MQ=255) gcgcTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑c > 1:137216/1‑149 (MQ=255) tAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAAc < 8:244349/105‑1 (MQ=255) tAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAAc > 7:244349/1‑105 (MQ=255) tAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGTACCG‑CCCAt > 6:158252/1‑149 (MQ=255) aaCCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAg < 8:61969/95‑1 (MQ=255) aaCCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAg > 7:61969/1‑95 (MQ=255) aaCCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATg < 3:114388/148‑1 (MQ=255) aCCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGAc < 3:182498/149‑1 (MQ=255) aCCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGAc < 5:245981/149‑1 (MQ=255) aGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCCCGcc > 3:248740/1‑149 (MQ=255) gCGTAAGGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTgg < 7:187141/148‑1 (MQ=255) aCGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGc < 4:125325/149‑1 (MQ=255) aCGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGc > 3:122636/1‑149 (MQ=255) aCGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGc > 3:125325/1‑149 (MQ=255) cGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTACGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCGCCCCATGACCAGCACGCCGTCGCCCTGGCCACGc < 1:197109/149‑1 (MQ=255) aggTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTACGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCGCCCCATGACCAGCACGCCGTCGCCCTGGCCACGc < 1:192787/148‑1 (MQ=255) ttGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACAG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAAc > 2:89543/1‑149 (MQ=255) ttCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGc < 4:122636/148‑1 (MQ=255) ttCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGcccc > 2:292887/1‑116 (MQ=255) ttCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGcccc < 1:292887/116‑1 (MQ=255) aTGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGTACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGgcgc < 5:158252/148‑1 (MQ=255) gCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATTATTCCAGCCGATTTTTGAGTGATTTGCCCCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGcc > 1:62732/1‑148 (MQ=255) aGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACt > 3:289904/1‑149 (MQ=255) ggTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTc < 2:137216/149‑1 (MQ=255) ccTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGc > 2:286703/1‑149 (MQ=255) | GCAGGCGGGTTGACGTTTGATCAACGGGCATCAGGAAACAGGCCTGCTTGCCACTGCGCTAAACCCAAAAAAGGGACATGAAGCGTCACGGTGCCTCCTTGTATTTTTTGTTCAGGCAGTGAGTTCATGCTAGGTTTCCACTGGCCCTGCGCCAAGGTACGAACTGCACCATT‑TTCCAGCCGATTTTTGAGTGATTTGCACCG‑CCCATGACCAGCACGCCGTCGCCCTGGCCACGCCCCTCGGAAACCGTTCGCGAGCTTATGCGCAAAGGCGCCGAACTCGCTCAGGCGCTGC > NC_002947/3210758‑3211051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |