| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,247,880 | G→A | 11.4% | G46S (GGC→AGC) | ureA → | urease subunit gamma |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,247,880 | 0 | G | A | 11.4% | 88.1 / 3.8 | 35 | G46S (GGC→AGC) | ureA | urease subunit gamma |
| Reads supporting (aligned to +/- strand): ref base G (16/15); new base A (2/2); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGAAGCGACCTTCCCGGATGGCACCAAGTTGGT > NC_002947/3247732‑3248022 | cTGCGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAg < 6:225837/149‑1 (MQ=255)cTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAg < 4:182389/149‑1 (MQ=255) agagCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGcc > 1:179216/1‑149 (MQ=255) cACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCcgc > 6:118262/1‑148 (MQ=255) ccATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGAt < 5:43943/149‑1 (MQ=255) gAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGcc < 4:178958/148‑1 (MQ=255) gCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGca < 5:118262/149‑1 (MQ=255) cGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAAGCGCCCGCGATGGCCGCACAGTCGcc > 6:228009/1‑149 (MQ=255) cGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAAGCGCCCGCGATGGCCGCACAGTCGcc > 4:168428/1‑149 (MQ=255) aCAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGc < 2:179216/149‑1 (MQ=255) gctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCAGGGGCGTg < 6:241045/149‑1 (MQ=255) cACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCATGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGc > 2:140235/1‑149 (MQ=255) gCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGAc > 6:14628/1‑149 (MQ=255) ccGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGAcc > 6:270720/1‑149 (MQ=255) gctgctGGAGGAGCGGCGCCTGGCCCGTGGCCTGAAGCACAACAACCCGGAGGCCCTGGCGCTGATCAGTGCCGCGGTGCTAGAAGGCGCCCGCGATCGCCGCACAGTCGCCTAACTGATGAGCATGGGGCGTGAAGTGCTGACCCGt > 4:281993/1‑148 (MQ=255) ctgctgGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAAGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGa < 3:168428/149‑1 (MQ=255) ctgctgGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGAGCAGTGCCGCGGTGCTCGAAAGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGa < 5:228009/149‑1 (MQ=255) ctgGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAg > 8:53540/1‑147 (MQ=255) ctgGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAg > 2:235066/1‑147 (MQ=255) ctgGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCa < 5:270720/149‑1 (MQ=255) tgGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGCCCCGTGAGCAg > 6:56472/1‑149 (MQ=255) ggCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAgg > 6:228405/1‑149 (MQ=255) gCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGt > 8:178898/1‑149 (MQ=255) gcggcTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCg > 3:111440/1‑149 (MQ=255) gCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCg < 7:33143/148‑1 (MQ=255) tGGCCTGAAGCTCAACTAGCCGGAGGCGGTGGCTCTGATCAGTGCCGCGGTGCTCGAAGTCGCCCGCGATGGCCGCACAGTCGCGGAACTGATGAGCCTGGTGCGTTAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGaaa < 3:281993/149‑1 (MQ=255) gCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAAt < 4:111440/148‑1 (MQ=255) gCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTc < 5:153286/73‑1 (MQ=255) gCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTc > 6:153286/1‑73 (MQ=255) tCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGaa < 8:274845/98‑1 (MQ=255) tCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGaa > 7:274845/1‑98 (MQ=255) cTACCCGGAGGCCATGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGc < 1:140235/149‑1 (MQ=255) cGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCACCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGAAGCGa > 1:84667/1‑149 (MQ=255) tCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATTTCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGAAGCGACCTTCCCGGAt < 1:235066/149‑1 (MQ=255) tGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGAAGCGACCTTCCCGGATGGCa > 7:143837/1‑149 (MQ=255) cTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGAAGCGACCTTCCCGGATGGCACCAAGTTGGt < 7:178898/149‑1 (MQ=255) cTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGAAGCGACCTTCCCGGATGGCACCAAGTTGGt < 7:53540/149‑1 (MQ=255) | CTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTGGCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGAAGCGACCTTCCCGGATGGCACCAAGTTGGT > NC_002947/3247732‑3248022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |