Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,271,146 G→C 14.6% R313R (CGC→CGG) ‡ PP_2869 ← FMN‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,271,1460GC14.6% 105.4 / 8.6 41R313R (CGC→CGG) ‡PP_2869FMN‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (16/19);  new base C (2/4);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGTGGCACGATATGGAC  >  NC_002947/3271002‑3271286
                                                                                                                                                |                                                                                                                                            
cAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCggg                                                                                                                                          <  5:26780/149‑1 (MQ=255)
      aCGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAgg                                                                                                                                     >  1:264834/1‑148 (MQ=255)
      aCGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCCGCGGCCAggc                                                                                                                                    <  8:148605/149‑2 (MQ=255)
       cGATTGAAATGGCCGATGCGTGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCCGCGGCCAggct                                                                                                                                   <  5:20354/149‑3 (MQ=255)
       cGATGGTAATGGCCGATGCAGGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCGCGGGTCATGCCGCACACATCAGCCTGGCCCGCGGCCAggct                                                                                                                                   <  5:181328/149‑3 (MQ=255)
        gATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATc                                                                                                                                  <  2:253076/149‑1 (MQ=255)
          tGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAg                                                                                                                                >  1:162397/1‑149 (MQ=255)
            gAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCATCCTGGCCCGCGGCCAgg                                                                                                                                     >  6:14498/1‑142 (MQ=255)
            gAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCATCCTGGCCCGCGGCCAgg                                                                                                                                     <  5:14498/142‑1 (MQ=255)
                     gATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCt                                                                                                                     <  5:35101/149‑1 (MQ=255)
                            gcCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAg                                                                                                                                      <  8:70190/125‑1 (MQ=255)
                            gcCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAg                                                                                                                                      >  7:70190/1‑125 (MQ=255)
                                 gTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGa                                                                                                         <  4:47081/149‑1 (MQ=255)
                                      aGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTACGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGTGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGATGGTTGATgcgc                                                                                                    >  6:102115/1‑149 (MQ=255)
                                               aGGCGCGCACGTCTTCGCCCTGGCCGCGTTCGGGCTTCGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGCCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACg                                                                                           <  2:264834/149‑1 (MQ=255)
                                               aGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGCCGGATCGCAGCTCAGCGCCCGGGTCATGCCGCAAACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACg                                                                                           >  1:12996/1‑149 (MQ=255)
                                               aGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGAc                                                                                            >  2:143160/1‑148 (MQ=255)
                                               aGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACg                                                                                           <  3:43916/149‑1 (MQ=255)
                                                   gcgcACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTgg                                                                                                                   <  2:222746/121‑1 (MQ=255)
                                                       aCGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATg                                                                                                       <  3:268672/129‑1 (MQ=255)
                                                       aCGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATg                                                                                                       >  4:268672/1‑129 (MQ=255)
                                                                              ggTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTc                                                                                                                       >  5:96762/1‑90 (MQ=255)
                                                                              ggTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTc                                                                                                                       <  6:96762/90‑1 (MQ=255)
                                                                                              gACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGc                                                                   <  6:147547/126‑1 (MQ=255)
                                                                                              gACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGc                                                                   >  5:147547/1‑126 (MQ=255)
                                                                                                      gCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCa                                                       <  4:264728/130‑1 (MQ=255)
                                                                                                      gCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCa                                                       >  3:264728/1‑130 (MQ=255)
                                                                                                              gcgcCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGt                                                           <  6:67007/118‑1 (MQ=255)
                                                                                                                gcCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGAt                          >  4:123719/1‑149 (MQ=255)
                                                                                                                   cGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGc                       <  2:12996/149‑1 (MQ=255)
                                                                                                                     ggTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGcc                      >  5:97482/1‑148 (MQ=255)
                                                                                                                       tCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATc                   <  1:143160/149‑1 (MQ=255)
                                                                                                                          tGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGc                                   <  4:116272/130‑1 (MQ=255)
                                                                                                                          tGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGc                                   >  3:116272/1‑130 (MQ=255)
                                                                                                                            ccGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGTg               <  3:123719/148‑1 (MQ=255)
                                                                                                                                  acaTCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGTGGCACGat        <  2:162397/149‑1 (MQ=255)
                                                                                                                                    aTCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGt                >  7:43048/1‑139 (MQ=255)
                                                                                                                                    aTCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGt                <  8:43048/139‑1 (MQ=255)
                                                                                                                                       atccTGGCCCGCGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGTGGCACGATATGGa   >  1:73565/3‑149 (MQ=255)
                                                                                                                                        gCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGTGGCACGATATGGAc  >  8:8470/1‑149 (MQ=255)
                                                                                                                                        gCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGTGGCACGATATGGAc  >  8:99433/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                            
CAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGTCTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATAGCCAGCTGTTGCTTGAAGGTGCCAGCCTCGCGGGCCAGGTACGCCGGTTCGATGGCCATCGGTGGCACGATATGGAC  >  NC_002947/3271002‑3271286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑