Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,369,023 C→T 8.7% L777L (CTG→TTG)  PP_2964 → transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,369,0230CT 8.7% 126.8 / 2.4 46L777L (CTG→TTG) PP_2964transposase
Reads supporting (aligned to +/- strand):  ref base C (23/19);  new base T (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01

TTCATCCCGCCGGGCATCTGGGAGGCGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAGGTCGCAT  >  NC_002947/3368876‑3369170
                                                                                                                                                   |                                                                                                                                                   
ttCATCCCGCCGGGCATCTGGGAGGCGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCt                                                                                                                                                    >  4:222270/1‑149 (MQ=255)
   aTCCCGCCGGGCATCTGGGAGGCGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGAt                                                                                                                                                 >  2:278702/1‑149 (MQ=255)
    tCCCGCCGGGCATCTGGGAGGCGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATg                                                                                                                                                >  5:101129/1‑149 (MQ=255)
    tCCCGCCGGGCATCTGGGAGGCGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATg                                                                                                                                                <  4:222017/149‑1 (MQ=255)
               aTCTGGGAGGCGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGACACGTATTct                                                                                                                                     >  6:35745/1‑148 (MQ=255)
                         cGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGgcgc                                                                                                                           <  4:300125/149‑1 (MQ=255)
                                        aGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTgc                                                                                                            >  4:230300/1‑149 (MQ=255)
                                          gggCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTgccg                                                                                                          >  6:167951/1‑149 (MQ=255)
                                                ctCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTgccggccg                                                                                                      <  1:278702/147‑1 (MQ=255)
                                                  cAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATc                                                                                                  >  6:56358/1‑149 (MQ=255)
                                                     ggCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATcgcg                                                                                               <  8:287350/149‑1 (MQ=255)
                                                      gCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCgg                                                                                              >  8:150908/1‑149 (MQ=255)
                                                      cttGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATcgcg                                                                                               <  5:266076/146‑1 (MQ=255)
                                                         gATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATcgcc                                                                                               >  6:266076/1‑144 (MQ=255)
                                                          aTCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTg                                                                                            <  8:83903/147‑1 (MQ=255)
                                                               accGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCAACGCTGTTTGACTTCACCCATCTGATGGGCATCAATCTGATGCCACGTATTCGAAACTGGCGCGACCTGGTGATTTGCAGGCCGGATCGCCGTTCTTCGTa                                                                                     >  8:66903/3‑149 (MQ=255)
                                                                 cGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTAt                                                                                                                                        >  2:102782/1‑96 (MQ=255)
                                                                   tCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAg                                                                                 <  5:56358/149‑1 (MQ=255)
                                                                        gCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCatat                                                                            <  7:150908/149‑1 (MQ=255)
                                                                        gCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCata                                                                             <  5:35745/148‑1 (MQ=255)
                                                                           gACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGtg                                                                                     >  1:106542/1‑136 (MQ=255)
                                                                           gACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTa                                                                                     <  2:106542/137‑1 (MQ=255)
                                                                                       tCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCAc                                                             >  3:112762/1‑149 (MQ=255)
                                                                                             aCCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGacac                                                       >  6:301790/1‑149 (MQ=255)
                                                                                               ccAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACaa                                                                                  <  8:39311/120‑1 (MQ=255)
                                                                                               ccAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACaa                                                                                  >  7:39311/1‑120 (MQ=255)
                                                                                               ccAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTg                                                     >  2:92168/1‑149 (MQ=255)
                                                                                                 aaGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGcc                                                   <  5:301790/149‑1 (MQ=255)
                                                                                                           ggCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACt                                         <  5:167951/149‑1 (MQ=255)
                                                                                                                cGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATc                                    <  3:230300/149‑1 (MQ=255)
                                                                                                                  gtgtTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCg                                   >  8:230089/1‑148 (MQ=255)
                                                                                                                          cTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTGCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACt                          <  4:112762/149‑1 (MQ=255)
                                                                                                                             cACCCATCTGTTGTGCATCTATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTg                         <  7:66903/147‑1 (MQ=255)
                                                                                                                                ccATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAg                     >  5:279919/1‑148 (MQ=255)
                                                                                                                                       cgtggCATCAGATTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGAt             <  2:227404/146‑1 (MQ=255)
                                                                                                                                        gtggCATCAGATTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGaa                                 <  2:301692/126‑1 (MQ=255)
                                                                                                                                        gtggCATCAGATTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGaa                                 >  1:301692/3‑128 (MQ=255)
                                                                                                                                        gtggCATCAGATTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATg            >  5:294343/3‑149 (MQ=255)
                                                                                                                                           gCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTg               >  2:244713/1‑143 (MQ=255)
                                                                                                                                           gCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTg               <  1:244713/143‑1 (MQ=255)
                                                                                                                                            cATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAgg        <  6:54994/149‑1 (MQ=255)
                                                                                                                                                aaTCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAGGTCg     <  6:279919/148‑1 (MQ=255)
                                                                                                                                                 aTCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAGGTCGCa   >  4:264355/1‑149 (MQ=255)
                                                                                                                                                  tCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAGGTCGCAt  >  6:142886/1‑149 (MQ=255)
                                                                                                                                                  tCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAGGTCGCAt  >  7:63731/1‑149 (MQ=255)
                                                                                                                                                  tCTGATGCCACGTATTCGCAACTGGAGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAGGTCGCAt  >  3:33001/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCATCCCGCCGGGCATCTGGGAGGCGATCTACGTGATCGAGGGGCTGCTCAAGGCTGATCTCAGCGTCGAGGCTGACACGGTGTACTCCGATACCCAAGGGCAGTCGGCCACGGTGTTTGCCTTCACCCATCTGTTGGGCATCAATCTGATGCCACGTATTCGCAACTGGCGCGACCTGGTGATGTGCCGGCCGGATCGCGGTGCTTCGTACAAGCATATCAACCGCCTGTTCACCGACACTGCCGACTGGAACTTGATCGAAACCCACTGGCAGGATCTGATGCAGGTCGCAT  >  NC_002947/3368876‑3369170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑