Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,402,506 G→C 9.1% intergenic (+30/‑122) PP_3010 → / → PP_3011 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,402,5060GC 9.1% 152.4 / 3.1 52intergenic (+30/‑122)PP_3010/PP_3011hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base C (2/3);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

AGGGGCTTGTAGAGTTGCGGGGCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATG  >  NC_002947/3402358‑3402640
                                                                                                                                                    |                                                                                                                                      
agGGGCTTGTAGAGTTGCGGGGCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCccgccg                                                                                                                                        >  4:190495/1‑149 (MQ=255)
                gCGGGGCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCTCTCGACGGCGGGCtat                                                                                                                         <  3:125825/148‑3 (MQ=255)
                    ggCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtgtg                                                                                                                    >  1:230415/1‑149 (MQ=255)
                     gCACGGGAAGCGAGCGCCACTTCAGGGCCTATCAACGTCATGCCTTGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTTATCATCCAACCCGCACACGAATAGCCCGCCGTCGTGAGCGGGCTTTTtgtgt                                                                                                                   <  3:187453/149‑1 (MQ=255)
                     gCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAAACATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtgtgt                                                                                                                   >  1:5734/1‑149 (MQ=255)
                           gAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCAt                                                                                                             >  3:74170/1‑149 (MQ=255)
                               cgagcgCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTc                                                                                                                                      >  1:157785/1‑120 (MQ=255)
                               cgagcgCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTc                                                                                                                                      <  2:157785/120‑1 (MQ=255)
                                gagcgCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCt                                                                                                        <  2:230415/149‑1 (MQ=255)
                                     ccACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtg                                                                                                                      <  4:250114/130‑1 (MQ=255)
                                     ccACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtg                                                                                                                      >  3:250114/1‑130 (MQ=255)
                                      cACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCgg                                                                                                  >  7:268879/1‑149 (MQ=255)
                                           cACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAAc                                                                                              <  2:5734/148‑1 (MQ=255)
                                               gCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTc                                                                                          >  1:54201/1‑148 (MQ=255)
                                                      aaCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAg                                                                                  >  2:177622/1‑149 (MQ=255)
                                                      aaCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAg                                                                                  >  7:283108/1‑149 (MQ=255)
                                                      aaCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAg                                                                                  >  7:168697/1‑149 (MQ=255)
                                                           gATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGAgg                                                                             >  6:52007/1‑149 (MQ=255)
                                                            aTGCCTGGTACGACCATGGCAGGTTGCTTGATTCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCggg                                                                            <  7:99617/149‑1 (MQ=255)
                                                            aTGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCggg                                                                            <  1:212158/149‑1 (MQ=255)
                                                             tGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGt                                                                           <  1:177622/149‑1 (MQ=255)
                                                               ccTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTg                                                                          >  3:185428/1‑148 (MQ=255)
                                                                 tGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATTGCCTGCCCGGCAACGTTCAAAAAAAGGGc                                                                               <  6:26003/141‑1 (MQ=255)
                                                                 tGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATTGCCTGCCCGGCAACGTTCAAAAAAAGGGc                                                                               >  5:26003/1‑141 (MQ=255)
                                                                 tGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAg                                                                       >  7:98432/1‑149 (MQ=255)
                                                                    tACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCg                                                                    <  5:89565/149‑1 (MQ=255)
                                                                       gACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTa                                                                 >  5:77428/1‑149 (MQ=255)
                                                                          cATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATgg                                                              <  8:268879/149‑1 (MQ=255)
                                                                               cAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGa                                                         <  3:190495/149‑1 (MQ=255)
                                                                                aGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGag                                                        <  4:74170/149‑1 (MQ=255)
                                                                                               gcACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGAGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGc                                          <  4:185428/148‑1 (MQ=255)
                                                                                                cACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGc                                          <  5:52007/147‑1 (MQ=255)
                                                                                                  cTGATGTGTTCAATAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAATAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGGTGCTTTGCAttt                                      <  2:206675/149‑1 (MQ=255)
                                                                                                     aTGTGTTCAAAAGCTTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGc                                    <  8:283108/148‑1 (MQ=255)
                                                                                                        tgtTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGc                                >  8:258548/1‑149 (MQ=255)
                                                                                                         gtTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGcc                               >  1:233191/1‑149 (MQ=255)
                                                                                                          ttaaaaaGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCTGGTGGAGTCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc                              >  1:138840/4‑149 (MQ=255)
                                                                                                          ttCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc                              <  6:77428/149‑1 (MQ=255)
                                                                                                          ttCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCAGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc                              >  5:174100/1‑149 (MQ=255)
                                                                                                               aaGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCcccgccc                         >  2:265027/1‑149 (MQ=255)
                                                                                                                 gCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCcccgcccg                        >  1:111030/1‑148 (MQ=255)
                                                                                                                 gCGTAATAATACAACCCCCACAACAATAGCCAGCCGTCGAGAGCGGTCTTTTTGTGTGCCCATCGCCTGACCGCCAACGTTAAAAAAAAGGGCGGGTGCAGCCCGTATGGCCTGAGCAGCGATGCTTTGCATTTGCCGGCaccgccag                        >  2:111033/1‑146 (MQ=255)
                                                                                                                  cGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTa                                                                 <  2:256437/106‑1 (MQ=255)
                                                                                                                     aaTCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAata                   >  4:219136/1‑149 (MQ=255)
                                                                                                                             aaCCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGt           <  8:168697/149‑1 (MQ=255)
                                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTATTGGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCtt                                             <  5:181353/109‑1 (MQ=21)
                                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTATTGGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCtt                                             >  4:261025/6‑114 (MQ=21)
                                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTATTGGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCtt                                             <  3:261025/109‑1 (MQ=21)
                                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTAATGGTGTGCCCATCGCCTGCCCGGCAACGTACAAAAAAAGGGCGGGTGGAGCCCGAATGGCCTGAGCAGCGTTGCtt                                             >  6:181353/6‑114 (MQ=18)
                                                                                                                                 cccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCATCGTTCAAAAAAAGGGCGTGTGGAGCCGGTTTGGCCTGAGCATCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTAttt       <  1:111033/149‑1 (MQ=255)
                                                                                                                                 cccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTAttt       <  1:265027/149‑1 (MQ=255)
                                                                                                                                  ccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGGAACGTTGAAAAACAGGGCGGCTGGAGCCGGTATGGCCTGAGCAGAGTTGCTTTGCATTTGGCGGCCACGCCCGTAGTCTTCGTGGttggtg      >  8:121050/1‑144 (MQ=255)
                                                                                                                                        cAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtggatg  >  2:218172/1‑147 (MQ=255)
                                                                                                                                                 gccgTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg      <  7:53586/134‑1 (MQ=255)
                                                                                                                                                 gccgTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg      >  8:53586/1‑134 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
AGGGGCTTGTAGAGTTGCGGGGCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATG  >  NC_002947/3402358‑3402640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑