Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,402,511 G→C 9.1% intergenic (+35/‑117) PP_3010 → / → PP_3011 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,402,5110GC 9.1% 155.6 / 3.5 52intergenic (+35/‑117)PP_3010/PP_3011hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base C (2/3);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGGGCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATGGAGAGTCGCAGGTGGAA  >  NC_002947/3402374‑3402657
                                                                                                                                         |                                                                                                                                                  
gCGGGGCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCTCTCGACGGCGGGCtat                                                                                                                                          <  3:125825/148‑3 (MQ=255)
    ggCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtgtg                                                                                                                                     >  1:230415/1‑149 (MQ=255)
     gCACGGGAAGCGAGCGCCACTTCAGGGCCTATCAACGTCATGCCTTGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTTATCATCCAACCCGCACACGAATAGCCCGCCGTCGTGAGCGGGCTTTTtgtgt                                                                                                                                    <  3:187453/149‑1 (MQ=255)
     gCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAAACATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtgtgt                                                                                                                                    >  1:5734/1‑149 (MQ=255)
           gAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCAt                                                                                                                              >  3:74170/1‑149 (MQ=255)
                gagcgCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCt                                                                                                                         <  2:230415/149‑1 (MQ=255)
                     ccACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtg                                                                                                                                       >  3:250114/1‑130 (MQ=255)
                     ccACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTtg                                                                                                                                       <  4:250114/130‑1 (MQ=255)
                      cACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCgg                                                                                                                   >  7:268879/1‑149 (MQ=255)
                           cACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAAc                                                                                                               <  2:5734/148‑1 (MQ=255)
                               gCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTc                                                                                                           >  1:54201/1‑148 (MQ=255)
                                      aaCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAg                                                                                                   >  2:177622/1‑149 (MQ=255)
                                      aaCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAg                                                                                                   >  7:168697/1‑149 (MQ=255)
                                      aaCGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAg                                                                                                   >  7:283108/1‑149 (MQ=255)
                                           gATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGAgg                                                                                              >  6:52007/1‑149 (MQ=255)
                                            aTGCCTGGTACGACCATGGCAGGTTGCTTGATTCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCggg                                                                                             <  7:99617/149‑1 (MQ=255)
                                            aTGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCggg                                                                                             <  1:212158/149‑1 (MQ=255)
                                             tGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGt                                                                                            <  1:177622/149‑1 (MQ=255)
                                               ccTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTg                                                                                           >  3:185428/1‑148 (MQ=255)
                                                 tGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATTGCCTGCCCGGCAACGTTCAAAAAAAGGGc                                                                                                <  6:26003/141‑1 (MQ=255)
                                                 tGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATTGCCTGCCCGGCAACGTTCAAAAAAAGGGc                                                                                                >  5:26003/1‑141 (MQ=255)
                                                 tGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAg                                                                                        >  7:98432/1‑149 (MQ=255)
                                                    tACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCg                                                                                     <  5:89565/149‑1 (MQ=255)
                                                       gACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTa                                                                                  >  5:77428/1‑149 (MQ=255)
                                                          cATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATgg                                                                               <  8:268879/149‑1 (MQ=255)
                                                               cAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGa                                                                          <  3:190495/149‑1 (MQ=255)
                                                                aGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGag                                                                         <  4:74170/149‑1 (MQ=255)
                                                                               gcACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGAGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGc                                                           <  4:185428/148‑1 (MQ=255)
                                                                                cACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGc                                                           <  5:52007/147‑1 (MQ=255)
                                                                                  cTGATGTGTTCAATAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAATAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGGTGCTTTGCAttt                                                       <  2:206675/149‑1 (MQ=255)
                                                                                     aTGTGTTCAAAAGCTTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGc                                                     <  8:283108/148‑1 (MQ=255)
                                                                                        tgtTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGc                                                 >  8:258548/1‑149 (MQ=255)
                                                                                         gtTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGcc                                                >  1:233191/1‑149 (MQ=255)
                                                                                          ttaaaaaGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCTGGTGGAGTCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc                                               >  1:138840/4‑149 (MQ=255)
                                                                                          ttCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc                                               <  6:77428/149‑1 (MQ=255)
                                                                                          ttCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCAGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGccc                                               >  5:174100/1‑149 (MQ=255)
                                                                                               aaGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCcccgccc                                          >  2:265027/1‑149 (MQ=255)
                                                                                                 gCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCcccgcccg                                         >  1:111030/1‑148 (MQ=255)
                                                                                                 gCGTAATAATACAACCCCCACAACAATAGCCAGCCGTCGAGAGCGGTCTTTTTGTGTGCCCATCGCCTGACCGCCAACGTTAAAAAAAAGGGCGGGTGCAGCCCGTATGGCCTGAGCAGCGATGCTTTGCATTTGCCGGCaccgccag                                         >  2:111033/1‑146 (MQ=255)
                                                                                                  cGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTa                                                                                  <  2:256437/106‑1 (MQ=255)
                                                                                                     aaTCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAata                                    >  4:219136/1‑149 (MQ=255)
                                                                                                             aaCCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGt                            <  8:168697/149‑1 (MQ=255)
                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTATTGGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCtt                                                              >  4:261025/6‑114 (MQ=21)
                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTATTGGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCtt                                                              <  3:261025/109‑1 (MQ=21)
                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTATTGGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCtt                                                              <  5:181353/109‑1 (MQ=21)
                                                                                                              atgggcacacaAAAAGCCCGCTCTCGACGGCGGGCTAATGGTGTGCCCATCGCCTGCCCGGCAACGTACAAAAAAAGGGCGGGTGGAGCCCGAATGGCCTGAGCAGCGTTGCtt                                                              >  6:181353/6‑114 (MQ=18)
                                                                                                                 cccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCATCGTTCAAAAAAAGGGCGTGTGGAGCCGGTTTGGCCTGAGCATCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTAttt                        <  1:111033/149‑1 (MQ=255)
                                                                                                                 cccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTAttt                        <  1:265027/149‑1 (MQ=255)
                                                                                                                  ccACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGGAACGTTGAAAAACAGGGCGGCTGGAGCCGGTATGGCCTGAGCAGAGTTGCTTTGCATTTGGCGGCCACGCCCGTAGTCTTCGTGGttggtg                       >  8:121050/1‑144 (MQ=255)
                                                                                                                        cAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtggatg                   >  2:218172/1‑147 (MQ=255)
                                                                                                                                 gccgTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg                       <  7:53586/134‑1 (MQ=255)
                                                                                                                                 gccgTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTtg                       >  8:53586/1‑134 (MQ=255)
                                                                                                                                       gagaGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATGGAGAGTCGCAGGTGGaa  <  6:174100/149‑1 (MQ=255)
                                                                                                                                       gagaGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATGGAGAGTCGCAGGTGGaa  <  3:219136/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
GCGGGGCACGGGAAGCGAGCGCCACTTCACGGCCTATCAACGTGATGCCTGGTACGACCATGGCAGGTTGCTGGATGCTGCACTGATGTGTTCAAAAGCGTAATCATCCAACCCCCACACCAATAGCCCGCCGTCGAGAGCGGGCTTTTTGTGTGCCCATCGCCTGCCCGGCAACGTTCAAAAAAAGGGCGGGTGGAGCCGGTATGGCCTGAGCAGCGTTGCTTTGCATTTGCCGGCCCCGCCCGTAATATTCGTTGTATTTGGATGGAGAGTCGCAGGTGGAA  >  NC_002947/3402374‑3402657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑