Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,417,742 G→A 8.5% A100V (GCA→GTA)  PP_3030 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,417,7420GA 8.5% 127.0 / 2.3 47A100V (GCA→GTA) PP_3030hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/18);  new base A (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

GGCAGATGGGTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCATGATGGG  >  NC_002947/3417594‑3417887
                                                                                                                                                    |                                                                                                                                                  
ggcAGATGGGTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCctgctg                                                                                                                                                    >  5:116340/1‑149 (MQ=255)
 gcAGATGGGTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGAGGGGGGGAATAATGCCtgctgc                                                                                                                                                   >  8:282025/1‑149 (MQ=255)
               ataCCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGAc                                                                                                                                    >  6:165157/1‑149 (MQ=255)
                     cACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTg                                                                                                                              <  8:20842/149‑1 (MQ=255)
                           aGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGtttttt                                                                                                                        <  7:282025/149‑1 (MQ=255)
                              cTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTgcg                                                                                                                     <  1:203201/149‑1 (MQ=255)
                              cTGCGAGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTAGTTGTGGGGTATCAGTGCCTCGGCGGGCCTGCTGATGGGGGGAATAATGCCTCCTGCTTCGAA‑GCAGAGAATTTTTCTTTTTTgc                                                                                                                      >  4:255456/1‑148 (MQ=255)
                                 cgcgTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGATTAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCtt                                                                                                                  >  5:120751/1‑149 (MQ=255)
                                  gcgTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTc                                                                                                                 <  5:165157/149‑1 (MQ=255)
                                    gTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGa                                                                                                               >  2:213943/1‑149 (MQ=255)
                                     tGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGac                                                                                                              <  2:303349/149‑1 (MQ=255)
                                               ttttAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACt                                                                                                    >  1:19577/1‑149 (MQ=255)
                                                    gCGATTACCGTTGCATCAGCGGTCGGTGCCTCGCGCAGCTTGTTGTGGGTTATCAGTGCCTCGGCGGGGCTGCTGATTGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTcgc                                                                                                <  6:185757/148‑1 (MQ=255)
                                                     cGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGCCTTcgcg                                                                                               >  8:127112/1‑148 (MQ=255)
                                                     cGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTACTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTcgcg                                                                                               >  8:215624/1‑148 (MQ=255)
                                                     cGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTACTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTcgcg                                                                                               >  8:219840/1‑148 (MQ=255)
                                                       aTTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGacaaca                                                                                                          >  3:272554/1‑135 (MQ=255)
                                                                 cATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGa                                                                                  <  7:23677/149‑1 (MQ=255)
                                                                  aTCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAg                                                                                 >  4:254525/1‑149 (MQ=255)
                                                                      gcgggcggTGCCTCGCGCAGCTTGTTGTGGGGTCTCAGTGCCTCGGGGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGc                                                                             <  3:255456/149‑1 (MQ=255)
                                                                      gcgggcggTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGc                                                                             >  8:116737/1‑149 (MQ=255)
                                                                       cgggcggTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGcc                                                                            <  2:19577/149‑1 (MQ=255)
                                                                           cggTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCAc                                                                        >  4:63948/1‑149 (MQ=255)
                                                                             gTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACtt                                                                      <  6:116340/149‑1 (MQ=255)
                                                                             gTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACtt                                                                      <  7:116737/149‑1 (MQ=255)
                                                                                ccTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAg                                                                   >  3:153919/1‑149 (MQ=255)
                                                                                    gcgcAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAACCGGGAGTGGCCTCACTTCAGTCCt                                                               >  4:135981/1‑149 (MQ=255)
                                                                                     cgcAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCtg                                                              >  6:211470/1‑149 (MQ=255)
                                                                                     cgcAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTACTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGACTCAAGTGAGGccac                                                              <  7:219840/149‑3 (MQ=255)
                                                                                     cgcAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTACTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGACTCAAGTGAGGccac                                                              <  7:215624/149‑3 (MQ=255)
                                                                                          cTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGaa                                                                                        <  4:111229/118‑1 (MQ=255)
                                                                                          cTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGaa                                                                                        >  3:111229/1‑118 (MQ=255)
                                                                                          cTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTg                                                         >  3:132445/1‑149 (MQ=255)
                                                                                             gttgtGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTc                                                       <  3:254525/148‑1 (MQ=255)
                                                                                                 tgGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCgg                                                                                    >  4:70202/1‑115 (MQ=255)
                                                                                                 tgGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCgg                                                                                    <  3:70202/115‑1 (MQ=255)
                                                                                                  ggggTATCAGTGCCTCTGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTGCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACATGAGTCCTGTGCTGTCGCGTGc                                                 >  6:16107/1‑149 (MQ=255)
                                                                                                    ggTATCTGTTCGTCGGGGGGGCTGCTGATGGGGTGATTAATGCCTGCTGCTTCGCA‑GCAGTTTCTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGTAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGccc                                               <  4:153919/149‑1 (MQ=255)
                                                                                                    ggTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGccc                                               >  4:220353/1‑149 (MQ=255)
                                                                                                      tATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGc                                             >  4:137167/1‑149 (MQ=255)
                                                                                                       aTCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCt                                            >  2:131635/1‑149 (MQ=255)
                                                                                                        tCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCtt                                           <  3:220353/149‑1 (MQ=255)
                                                                                                           gTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTccc                                        >  2:234179/1‑149 (MQ=255)
                                                                                                              ccTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTcc                                     >  3:266171/1‑149 (MQ=255)
                                                                                                                  ggcggGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCggg                                  <  1:213943/148‑1 (MQ=255)
                                                                                                                   gcggGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGTCAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGt                                <  3:135981/149‑1 (MQ=255)
                                                                                                                            ctgATGGGGGGGATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTtg                       >  8:38587/1‑149 (MQ=255)
                                                                                                                            ctgATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTtg                       >  1:79095/1‑149 (MQ=255)
                                                                                                                               aTGGGGGGAATAATGCCTGCTGCTTCGCAGGCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCAGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTtgtg                     >  1:179915/1‑149 (MQ=255)
                                                                                                                                                  ctgctTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCATCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCATGATggg  >  3:234143/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCAGATGGGTGCGTATACCCCACGGCAGGCTGCGCGTGCGGGCGACTTTTAGCGATTAGCGTTGCATCAGCGGGCGGTGCCTCGCGCAGCTTGTTGTGGGGTATCAGTGCCTCGGCGGGGCTGCTGATGGGGGGAATAATGCCTGCTGCTTCGCA‑GCAGAGACTTTTTGTTTTTTGCGCTTCGACAACACGGACTTCGCGGAGCGAAGCGGGAGTGGCCTCACTTGAGTCCTGTGCTGTCGCGTGCCCGCTTCCCTCCGGGGTCAGGACTTGTGGTGGTATTCCCATGATGGG  >  NC_002947/3417594‑3417887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑