Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 274,154 A→T 9.5% L139Q (CTG→CAG)  PP_0221 ← methionine ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947274,1540AT 9.5% 175.6 / 7.6 63L139Q (CTG→CAG) PP_0221methionine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base T (2/4);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGCCTGCAGCAG  >  NC_002947/274007‑274301
                                                                                                                                                    |                                                                                                                                                   
tCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCcagcagcagcag                                                                                                                                                    <  3:201403/149‑1 (MQ=255)
   gCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTGGAGGGTGATCAAC‑CCGGCCGTGGCCTGCTGCTGCTGGcc                                                                                                                                                 <  1:290094/149‑1 (MQ=255)
     gtcgtcgtAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGTGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCTGCTGCTGCTGGCCaa                                                                                                                                               <  7:276723/149‑2 (MQ=255)
        gtcgtAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCGTGGCCTGCTGCTGCTGGCCAAGGc                                                                                                                                            <  4:242187/149‑1 (MQ=255)
         tcgtAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGcc                                                                                                                                           >  4:268061/1‑149 (MQ=255)
            tAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGNCTTGGCCAGCAGCAGCAGGCCACGGCCGCt                                                                                                                                        <  1:25636/149‑1 (MQ=255)
            tAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCt                                                                                                                                        <  1:30405/149‑1 (MQ=255)
            tAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCACACGCAGTGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCAACGGCCgat                                                                                                                                        >  8:268006/1‑147 (MQ=255)
              ggCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTg                                                                                                                                       <  8:219969/148‑1 (MQ=255)
               gcgcGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAACAGCGGCCGTGGCCTGCTGCTGCTGGCCAAGGCCgggtt                                                                                                                                      <  6:44819/149‑5 (MQ=37)
                      ccAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCggg                                                                                                                              <  5:83702/149‑1 (MQ=255)
                        aGTTGCACCGCGTCGAGCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTc                                                                                                                            <  3:173825/149‑1 (MQ=255)
                          ttGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGt                                                                                                                          <  5:25457/149‑1 (MQ=255)
                          ttGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGt                                                                                                                          >  6:25457/1‑149 (MQ=255)
                            gCACCGCCTCGACCTGAAGGATGTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGt                                                                                                                                      >  5:225632/1‑135 (MQ=255)
                            gCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGt                                                                                                                                      <  6:221409/135‑1 (MQ=255)
                            gCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGt                                                                                                                                      >  5:221409/1‑135 (MQ=255)
                            gCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGt                                                                                                                                      <  6:225632/135‑1 (MQ=255)
                            gCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTg                                                                                                                        >  6:8937/1‑149 (MQ=255)
                              aCCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTg                                                                                                                        >  7:278323/1‑147 (MQ=255)
                              aCCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTg                                                                                                                        <  8:278323/147‑1 (MQ=255)
                                 gCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGAt                                                                                                                   <  4:102276/149‑1 (MQ=255)
                                            aaGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTg                                                                                                         <  3:129468/148‑1 (MQ=255)
                                              ggATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGgcg                                                                                                      <  5:175724/149‑1 (MQ=255)
                                                 tCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCt                                                                                                   >  4:44042/1‑149 (MQ=255)
                                                  cTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCg                                                                                                                                          <  1:296800/109‑1 (MQ=255)
                                                  cTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCg                                                                                                                                          >  2:296800/1‑109 (MQ=255)
                                                     cAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAg                                                                                               >  7:274103/1‑149 (MQ=255)
                                                     cAGCTTTTTAGGGGTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCa                                                                                                <  3:205856/148‑1 (MQ=255)
                                                      aGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGTTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAgg                                                                                              <  8:161590/149‑1 (MQ=255)
                                                      aGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAgg                                                                                              <  3:44042/149‑1 (MQ=255)
                                                          ttttAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAg                                                                                           >  6:257474/1‑148 (MQ=255)
                                                                  ttGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCa                                                                                    >  4:228564/1‑147 (MQ=255)
                                                                        aGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCtttt                                                                            <  2:44525/149‑1 (MQ=255)
                                                                         ggATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTg                                                                           >  8:26669/1‑149 (MQ=255)
                                                                               cAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAg                                                                                 >  8:122823/1‑137 (MQ=255)
                                                                               cAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAg                                                                                 <  7:122823/137‑1 (MQ=255)
                                                                                  ccTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTc                                                                  <  4:266494/149‑1 (MQ=255)
                                                                                   cTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACtcgaaaa                                                                 <  3:268061/149‑7 (MQ=255)
                                                                                      ggTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGa                                                                <  5:277807/147‑1 (MQ=255)
                                                                                       gTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGTCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTa                                                             <  8:274103/149‑1 (MQ=255)
                                                                                       gTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTa                                                             <  5:8937/149‑1 (MQ=255)
                                                                                                    ccACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACgttg                                                >  3:45976/1‑149 (MQ=255)
                                                                                                    ccACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACgttg                                                >  2:243059/1‑149 (MQ=255)
                                                                                                          cGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtgatg                                          <  3:228564/149‑1 (MQ=255)
                                                                                                          cGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTtgat                                           >  3:211864/1‑148 (MQ=255)
                                                                                                               ttGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGcc                                     >  8:95426/1‑149 (MQ=255)
                                                                                                                   gggTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGGCCggg                                 >  7:206920/1‑149 (MQ=255)
                                                                                                                    ggTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCgggg                                >  7:10344/1‑149 (MQ=255)
                                                                                                                    ggTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCgggg                                >  5:162416/1‑149 (MQ=255)
                                                                                                                      tGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCg                              >  5:307105/1‑149 (MQ=255)
                                                                                                                        aTCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTAGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTGGATGAAGATGCCCGGGGCGt                             >  8:102086/1‑148 (MQ=255)
                                                                                                                         tCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCCCGCTGGCGCCGTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGGTGTGGATGATGCCCGGGGCGt                             >  5:296564/1‑147 (MQ=255)
                                                                                                                         tCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTaa                           <  6:307105/149‑1 (MQ=255)
                                                                                                                          cAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTaa                           <  4:45976/148‑1 (MQ=255)
                                                                                                                            acagcGGCCGTGGCGTGCTGCTGCTGGCCAAGG‑CGGGGTTGATCGGGTCGTTGGCGATGGCCCCGCTGGCGCCTTCAGGACGCTCGGCAAAGCTTTTGTAGTTTTTAGAGTACAGGCCAACGTTTTTGATGATGCCCGGGGCGTAAgg                         >  4:77456/5‑148 (MQ=255)
                                                                                                                            aC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGCCGATGGTTACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGc                        <  1:252754/149‑1 (MQ=255)
                                                                                                                             c‑ccGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGca                       >  6:134838/1‑149 (MQ=255)
                                                                                                                                 ggCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACaa                    <  7:95426/149‑1 (MQ=255)
                                                                                                                                 ggCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACaa                    <  8:10344/149‑1 (MQ=255)
                                                                                                                                   ccgtGGCCTGCTGCTGCTGGCCAAGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAgg                  >  2:280562/4‑149 (MQ=255)
                                                                                                                                   ccTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAgg                  >  8:71194/1‑149 (MQ=255)
                                                                                                                                    cTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGt                 <  2:236658/149‑1 (MQ=255)
                                                                                                                                    cTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGt                 >  8:35587/1‑149 (MQ=255)
                                                                                                                                                 agcagGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGCCTgcag     >  3:112225/1‑148 (MQ=255)
                                                                                                                                                   cagGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGCCTGcagcag  >  1:172124/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCGGCGTCGTCGTAGGCGCGTACCAGTTGCACCGCCTCGACCTGAAGGATCTTCAGCTTTTTAGGGTTGGCGAGGATGTCAGCCTCGGTGGCCTTGTAGCCCACGCCGGGCTTGAGGGTGATCAAC‑CCGGCCTTGGCCAGCAGCAGCAGGCCACGGCCGCTGTTGATCGGGTCGTTGGCGATGGCCACGCTGGCGCCTTCAGGCAGCTCGGCAAAGCTTTTGTACTTTTTCGAGTACAGGCCAACGTTGTTGATGATGCCCGGGGCGTAAGGCACAAGGTTGAAGCCTGCAGCAG  >  NC_002947/274007‑274301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑