Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,441,436 G→C 7.7% A65A (GCG→GCC)  PP_3062 → tail length determination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,441,4360GC 7.7% 163.0 / 3.0 52A65A (GCG→GCC) PP_3062tail length determination protein
Reads supporting (aligned to +/- strand):  ref base G (26/22);  new base C (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTCGCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCAC  >  NC_002947/3441290‑3441580
                                                                                                                                                    |                                                                                                                                                
tcgtcgCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAgcggcgcggc                                                                                                                                                >  1:141966/1‑149 (MQ=255)
   tcgCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGcggccgg                                                                                                                                             <  4:173635/149‑1 (MQ=255)
    cgCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGcggccggc                                                                                                                                            >  5:81713/1‑149 (MQ=255)
     gCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGgcg                                                                                                                                           >  3:196263/1‑149 (MQ=255)
       tCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGcc                                                                                                                                         <  4:139272/149‑1 (MQ=255)
              cgcgTTCAAGACCGCCGAAAACGGCCTCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgtc                                                                                                                                   >  1:255082/1‑143 (MQ=255)
              cgcgTTCAAGACCGCCGAAAACGGCCTCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgtc                                                                                                                                   <  2:255082/148‑6 (MQ=255)
               gcgTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGtt                                                                                                                                 >  3:74281/1‑149 (MQ=255)
                     aaGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcg                                                                                                                           >  8:275412/1‑149 (MQ=255)
                      aGACCGCCGAAAACGGCATCCAGAAGCTGGGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcg                                                                                                                           >  1:22341/1‑149 (MQ=255)
                       gACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcgc                                                                                                                          >  4:2/1‑148 (MQ=255)
                        aCCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGCGCAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcgc                                                                                                                          >  1:222704/1‑148 (MQ=255)
                          cgccgAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGc                                                                                                                      >  3:41863/1‑149 (MQ=255)
                            ccgAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTg                                                                                                                    <  3:165822/149‑1 (MQ=255)
                             cgAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTg                                                                                                                    >  4:183009/1‑148 (MQ=255)
                               aaaaCGGCATCCAGACGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGAc                                                                                                                 <  5:201146/149‑1 (MQ=255)
                                  aCGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAg                                                                                                               >  2:295222/1‑148 (MQ=255)
                                    ggCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgt                                                                                                                                    <  7:259628/125‑5 (MQ=255)
                                    ggCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgt                                                                                                                                    >  8:259628/1‑121 (MQ=255)
                                       aTCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCt                                                                                                         <  6:81713/149‑1 (MQ=255)
                                         ccAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTg                                                                                                        >  1:303301/1‑148 (MQ=255)
                                            gAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACGAGTTGCTGCGCAAGCTGGACAGCAACCTGGAtt                                                                                                    <  3:14900/149‑1 (MQ=255)
                                             aaGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTc                                                                                                   <  1:33296/149‑1 (MQ=255)
                                             aaGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTc                                                                                                   >  2:33296/1‑149 (MQ=255)
                                                cT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGtt                                                                                                <  2:22341/149‑1 (MQ=255)
                                                    gAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTgc                                                                                              <  1:295222/148‑1 (MQ=255)
                                                            gggCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCaa                                                                                          >  7:221230/1‑144 (MQ=255)
                                                            gggCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCaa                                                                                          <  8:221230/144‑1 (MQ=255)
                                                             ggCAACAAGGC‑CAAGGTTCTTAAAAGCACCATTGGCTAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAggg                                                                                    <  4:196263/149‑1 (MQ=255)
                                                                    aGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAg                                                                             <  3:183009/149‑1 (MQ=255)
                                                                         cAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCg                                                                         >  5:86850/1‑149 (MQ=255)
                                                                           aGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGt                                                                       <  7:275412/149‑1 (MQ=255)
                                                                                cTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGc                                                                   <  2:222704/148‑1 (MQ=255)
                                                                                 tGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTc                                                                 >  3:19902/1‑149 (MQ=255)
                                                                                       gCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTcgc                                                           <  3:2/149‑1 (MQ=255)
                                                                                         acgaTTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGa                                                         <  8:123391/146‑1 (MQ=255)
                                                                                                gCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAg                                                  <  5:245062/149‑1 (MQ=255)
                                                                                                  gAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAg                                                        <  1:271577/141‑1 (MQ=255)
                                                                                                  gAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAg                                                        >  2:271577/1‑141 (MQ=255)
                                                                                                   aaaCCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAgcgc                                               >  4:219127/1‑149 (MQ=255)
                                                                                                         tCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTggg                                         >  5:17597/1‑149 (MQ=255)
                                                                                                            aGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGgcgc                                      <  4:74281/149‑1 (MQ=255)
                                                                                                             gCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGgcgc                                      >  6:255908/1‑148 (MQ=255)
                                                                                                                    gAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGCTTCGTTGCGCGAGCAGGGGATCGAGGGCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGcgcg                              >  4:191535/1‑149 (MQ=255)
                                                                                                                      ggAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCggg                            >  2:177164/1‑149 (MQ=255)
                                                                                                                      ggAATGGAAGCGCGCCCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCggg                            >  1:141432/1‑149 (MQ=255)
                                                                                                                        aaTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGgcc                          >  5:27433/1‑149 (MQ=255)
                                                                                                                         aTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCa                                                   >  6:167748/1‑123 (MQ=255)
                                                                                                                         aTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCa                                                   <  5:167748/123‑1 (MQ=255)
                                                                                                                         aTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGgccg                         >  1:30617/1‑149 (MQ=255)
                                                                                                                                  cgcgcACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTg                 >  5:80966/1‑148 (MQ=255)
                                                                                                                                       aCGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCt            >  4:467/1‑148 (MQ=255)
                                                                                                                                       aCGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTc           <  3:290801/149‑1 (MQ=255)
                                                                                                                                          aCAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAg        <  5:255908/149‑1 (MQ=255)
                                                                                                                                           cAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAgg       <  1:177164/149‑1 (MQ=255)
                                                                                                                                             gcggcgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGt     >  2:277137/1‑149 (MQ=255)
                                                                                                                                               ggcgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCACCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCa   <  2:303301/149‑1 (MQ=255)
                                                                                                                                                 cgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCAc  >  8:275755/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCGTCGCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCAC  >  NC_002947/3441290‑3441580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑