Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,441,440 G→C 7.3% G67R (GGC→CGC)  PP_3062 → tail length determination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,441,4400GC 7.3% 170.9 / 3.7 55G67R (GGC→CGC) PP_3062tail length determination protein
Reads supporting (aligned to +/- strand):  ref base G (28/23);  new base C (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

TCGCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCA  >  NC_002947/3441293‑3441585
                                                                                                                                                     |                                                                                                                                                 
tcgCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGcggccgg                                                                                                                                                  <  4:173635/149‑1 (MQ=255)
 cgCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGcggccggc                                                                                                                                                 >  5:81713/1‑149 (MQ=255)
  gCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGgcg                                                                                                                                                >  3:196263/1‑149 (MQ=255)
    tCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGcc                                                                                                                                              <  4:139272/149‑1 (MQ=255)
           cgcgTTCAAGACCGCCGAAAACGGCCTCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgtc                                                                                                                                        >  1:255082/1‑143 (MQ=255)
           cgcgTTCAAGACCGCCGAAAACGGCCTCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgtc                                                                                                                                        <  2:255082/148‑6 (MQ=255)
            gcgTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGtt                                                                                                                                      >  3:74281/1‑149 (MQ=255)
                  aaGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcg                                                                                                                                >  8:275412/1‑149 (MQ=255)
                   aGACCGCCGAAAACGGCATCCAGAAGCTGGGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcg                                                                                                                                >  1:22341/1‑149 (MQ=255)
                    gACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcgc                                                                                                                               >  4:2/1‑148 (MQ=255)
                     aCCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGCGCAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTgcgc                                                                                                                               >  1:222704/1‑148 (MQ=255)
                       cgccgAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGc                                                                                                                           >  3:41863/1‑149 (MQ=255)
                         ccgAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTg                                                                                                                         <  3:165822/149‑1 (MQ=255)
                          cgAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTg                                                                                                                         >  4:183009/1‑148 (MQ=255)
                            aaaaCGGCATCCAGACGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGAc                                                                                                                      <  5:201146/149‑1 (MQ=255)
                               aCGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAg                                                                                                                    >  2:295222/1‑148 (MQ=255)
                                 ggCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgt                                                                                                                                         <  7:259628/125‑5 (MQ=255)
                                 ggCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCCGGCCGCGCCgctgt                                                                                                                                         >  8:259628/1‑121 (MQ=255)
                                    aTCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCt                                                                                                              <  6:81713/149‑1 (MQ=255)
                                      ccAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTg                                                                                                             >  1:303301/1‑148 (MQ=255)
                                         gAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACGAGTTGCTGCGCAAGCTGGACAGCAACCTGGAtt                                                                                                         <  3:14900/149‑1 (MQ=255)
                                          aaGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTc                                                                                                        >  2:33296/1‑149 (MQ=255)
                                          aaGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTc                                                                                                        <  1:33296/149‑1 (MQ=255)
                                             cT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGtt                                                                                                     <  2:22341/149‑1 (MQ=255)
                                                 gAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTgc                                                                                                   <  1:295222/148‑1 (MQ=255)
                                                         gggCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCaa                                                                                               <  8:221230/144‑1 (MQ=255)
                                                         gggCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCaa                                                                                               >  7:221230/1‑144 (MQ=255)
                                                          ggCAACAAGGC‑CAAGGTTCTTAAAAGCACCATTGGCTAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAggg                                                                                         <  4:196263/149‑1 (MQ=255)
                                                                 aGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAg                                                                                  <  3:183009/149‑1 (MQ=255)
                                                                      cAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCg                                                                              >  5:86850/1‑149 (MQ=255)
                                                                        aGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGt                                                                            <  7:275412/149‑1 (MQ=255)
                                                                             cTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGc                                                                        <  2:222704/148‑1 (MQ=255)
                                                                              tGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTc                                                                      >  3:19902/1‑149 (MQ=255)
                                                                                    gCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTcgc                                                                <  3:2/149‑1 (MQ=255)
                                                                                      acgaTTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGa                                                              <  8:123391/146‑1 (MQ=255)
                                                                                             gCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAg                                                       <  5:245062/149‑1 (MQ=255)
                                                                                               gAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAg                                                             <  1:271577/141‑1 (MQ=255)
                                                                                               gAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAg                                                             >  2:271577/1‑141 (MQ=255)
                                                                                                aaaCCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAgcgc                                                    >  4:219127/1‑149 (MQ=255)
                                                                                                      tCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTggg                                              >  5:17597/1‑149 (MQ=255)
                                                                                                         aGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGgcgc                                           <  4:74281/149‑1 (MQ=255)
                                                                                                          gCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGgcgc                                           >  6:255908/1‑148 (MQ=255)
                                                                                                                 gAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGCTTCGTTGCGCGAGCAGGGGATCGAGGGCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGcgcg                                   >  4:191535/1‑149 (MQ=255)
                                                                                                                   ggAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCggg                                 >  2:177164/1‑149 (MQ=255)
                                                                                                                   ggAATGGAAGCGCGCCCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCggg                                 >  1:141432/1‑149 (MQ=255)
                                                                                                                     aaTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGgcc                               >  5:27433/1‑149 (MQ=255)
                                                                                                                      aTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCa                                                        <  5:167748/123‑1 (MQ=255)
                                                                                                                      aTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCa                                                        >  6:167748/1‑123 (MQ=255)
                                                                                                                      aTGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGgccg                              >  1:30617/1‑149 (MQ=255)
                                                                                                                               cgcgcACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTg                      >  5:80966/1‑148 (MQ=255)
                                                                                                                                    aCGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCt                 >  4:467/1‑148 (MQ=255)
                                                                                                                                    aCGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTc                <  3:290801/149‑1 (MQ=255)
                                                                                                                                       aCAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAg             <  5:255908/149‑1 (MQ=255)
                                                                                                                                        cAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAgg            <  1:177164/149‑1 (MQ=255)
                                                                                                                                          gcggcgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGt          >  2:277137/1‑149 (MQ=255)
                                                                                                                                            ggcgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCACCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCa        <  2:303301/149‑1 (MQ=255)
                                                                                                                                              cgcggcCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCAc       >  8:275755/1‑148 (MQ=255)
                                                                                                                                                  gccggcGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACcagca  <  2:141432/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCGCTCGGCGCCGCGTTCAAGACCGCCGAAAACGGCATCCAGAAGCT‑GGAAGCCAAGGGCAACAAGGC‑CAAGGTGCTGAAAAGCACCATTGGCGAAACCATCAAGCTGCGCGAGGAATGGAAGCGCGCGCACGACAGCGGCGCGGCCGGCGCCGACAAGTTGCTGCGCAAGCTGGACAGCAACCTGGATTCGTTGCGCAAGCAGGGGATCGAGGTCGGTAAGCTCGGTCGCGAGTACCAGCGCCTTGGGCGCGAGGCGCGGGCCGCCGATCTGCAGCTCAAGGGTCACCAGCA  >  NC_002947/3441293‑3441585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑