| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,466,841 | T→G | 33.2% | intergenic (‑16/+108) | PP_3081 ← / ← PP_5560 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,466,841 | 0 | T | G | 33.2% | 12.1 / 10.1 | 21 | intergenic (‑16/+108) | PP_3081/PP_5560 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/10); new base G (4/3); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01 | |||||||||||
CGGGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTGGCTC > NC_002947/3466702‑3466989 | cGGGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGGCGGccgcgggc > 8:184724/1‑147 (MQ=255) gggCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCAACATCGCGCCTTGGCCGCCGCAGGCGGCCa < 2:96848/149‑1 (MQ=255) gTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCtgtg > 8:208470/1‑148 (MQ=255) cAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAggg < 7:95697/149‑1 (MQ=255) ggggTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCCGCCCACGCTGTGCAGGGCAGCAAaccagc > 4:67572/1‑149 (MQ=255) gTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGaa < 3:67572/149‑1 (MQ=255) ccGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTTAAAGACGAGCAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACa < 7:184724/149‑1 (MQ=255) aCCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGt < 3:207899/149‑1 (MQ=255) ccGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGGGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGGGAACAGCCAGTGTGTGAGGTg > 8:216063/1‑149 (MQ=255) aggcggggTCCTGGCCGACCTGTTGCTGGGCAATGACGCCGCCATCAGTTAATGACGAGGAACTGCCCGAGATCGCGCCTTGGCGGCCTGGGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATg < 1:125449/144‑1 (MQ=255) ggTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAAt < 1:15740/149‑1 (MQ=255) ccTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGc < 7:208470/149‑1 (MQ=255) ccTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCAGCGGCGGCCCACGCGGTGCAGGGCAGCAAACCAGCGCACAGCCAGTGTCGGGGGTGCATGGTCAATCCTTGGCAGGGc > 8:12862/1‑149 (MQ=255) cgcaATCAGTTAAACACTAGTAACTGACCTACATCGCGCCTTGGCCGCGTGCGGGGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCa < 2:302837/149‑1 (MQ=255) tccgTGAAACACTAGGAACTGACCGACATCGCGCCTGGGCCGCCGGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCt < 2:134354/146‑1 (MQ=255) gAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGCCTAGCCCAGGTCTGCTTCAGCGGCt < 6:174984/149‑1 (MQ=255) cTGACCGACATCGCGCCTTGGCCGCCGGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTg < 3:147436/149‑1 (MQ=255) agcgttGGCCGCCGCAGGCGGCCAAGGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCGGGTCCTCGGCAGTg > 3:44836/5‑149 (MQ=255) agcgttGGCCGCCGCAGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGGCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTg > 4:183970/5‑149 (MQ=255) cgttGGCCGCCGCAGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGcc > 1:122157/3‑149 (MQ=255) ggCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTc < 3:36047/147‑1 (MQ=255) tGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTGGCTc > 8:81921/1‑149 (MQ=255) | CGGGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTCCGCTGGCTC > NC_002947/3466702‑3466989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |