Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,587,358 T→C 8.1% L65P (CTG→CCG)  catA‑II → catechol 1,2‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,587,3580TC 8.1% 134.3 / 2.7 50L65P (CTG→CCG) catA‑IIcatechol 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (19/26);  new base C (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GGCCGCAGGCTTTTGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTACGTGGCT  >  NC_002947/3587215‑3587500
                                                                                                                                                |                                                                                                                                              
ggCCGCAGGCTTTTGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCg                                                                                                                                           <  2:267502/149‑1 (MQ=255)
    gCAGGCTTTTGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTcg                                                                                                                                       >  1:212565/1‑149 (MQ=255)
        gcgtttGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGTAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCgcggc                                                                                                                                   <  4:237712/146‑1 (MQ=255)
          ttttGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGc                                                                                                                                            <  8:260759/138‑1 (MQ=255)
          ttttGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGc                                                                                                                                            >  7:260759/1‑138 (MQ=255)
            ttGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGa                                                                                                                                 <  8:225256/147‑1 (MQ=255)
                 aTGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCgg                                                                                                                           <  5:300921/148‑1 (MQ=255)
                    cggccggcAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACCTGCCTGGGCGGTCGCGGCGAAGCCggg                                                                                                                          <  1:2867/147‑1 (MQ=255)
                     ggccggcAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGc                                                                                                                      >  6:122533/1‑149 (MQ=255)
                      gccggcAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCt                                                                                                                     <  2:212565/149‑1 (MQ=255)
                      gccggcAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCCAGGCGGttgag                                                                                                                                     >  3:121609/1‑129 (MQ=255)
                      gccggcAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCCAGGCGGttgag                                                                                                                                     <  4:121609/133‑5 (MQ=255)
                       ccggcAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCtg                                                                                                                    <  5:238726/149‑1 (MQ=255)
                                    ccTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTgg                                                                                                       <  4:95738/149‑1 (MQ=255)
                                    ccTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCCGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTgg                                                                                                       <  6:159185/149‑1 (MQ=255)
                                     cTCACACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGGTCTGGCACGCCGTCGATTACCTCAACC‑GCCGGGGCGGTCGCGGCGCCGCCGGGTGGCtggtgg                                                                                                                >  8:248574/1‑139 (MQ=255)
                                     cTCAACCGCCGCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGCCATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCACCC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCtggtgg                                                                                                                <  7:248574/139‑1 (MQ=37)
                                                                cAGGTTACCGCGCGTCTGATCGAAGACCTGGACATCAGGGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAAGC‑GGCTGGGCGGTCGCGGGGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATAGAACACTTCCTCGACctgctg                                                                             <  4:130366/147‑1 (MQ=25)
                                                                cAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACctgctg                                                                             >  4:244725/1‑147 (MQ=255)
                                                                cAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACctgctg                                                                             >  8:89208/1‑147 (MQ=255)
                                                                  ggATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAg                                                                          >  6:53847/1‑148 (MQ=255)
                                                                   gATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGa                                                                        >  2:98093/1‑149 (MQ=255)
                                                                   gATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGa                                                                        >  3:193381/1‑149 (MQ=255)
                                                                   gATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGa                                                                        >  3:206925/1‑149 (MQ=255)
                                                                     tACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATg                                                                      <  3:288954/149‑1 (MQ=255)
                                                                           gcgGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAgg                                                                <  7:89208/149‑1 (MQ=255)
                                                                             ggCTGATCGAAGACCTGTACATCAGCGAATACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCTAAGACTGGTTGCTGGTGGCGGGGCTGGTCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAATGAc                                                              <  5:66283/149‑1 (MQ=25)
                                                                             ggCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTCCCTCAACC‑GCCTGGGCGGTCGCGGCGAAGGCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGAc                                                              <  5:70706/149‑1 (MQ=255)
                                                                                 gATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCag                                                           <  5:184463/148‑1 (MQ=255)
                                                                                 gATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCag                                                           >  6:184463/1‑148 (MQ=255)
                                                                                        gACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGATGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAgg                                                   <  1:98093/149‑1 (MQ=255)
                                                                                           cTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGgcg                                                >  5:295687/1‑149 (MQ=255)
                                                                                              gACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGt                                             <  5:122533/149‑1 (MQ=255)
                                                                                                 aTCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTgg                                          >  5:136786/1‑149 (MQ=255)
                                                                                                   cAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTggcg                                        >  6:216216/1‑149 (MQ=255)
                                                                                                    aGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAggg                                                  >  1:168707/1‑138 (MQ=255)
                                                                                                    aGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAggg                                                  <  2:168707/138‑1 (MQ=255)
                                                                                                       gAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCAc                                    <  3:261346/149‑1 (MQ=255)
                                                                                                            cGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCa                                <  1:106959/148‑1 (MQ=255)
                                                                                                             gAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTggc                                         <  2:286189/138‑1 (MQ=255)
                                                                                                             gAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTggc                                         >  1:286189/1‑138 (MQ=255)
                                                                                                                       aCGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGaa                    <  3:244725/149‑1 (MQ=255)
                                                                                                                         gCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAgg                  >  7:273976/1‑149 (MQ=255)
                                                                                                                             tCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACCCTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCg              >  8:229661/1‑149 (MQ=255)
                                                                                                                                    ccTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTAc        <  6:295687/148‑1 (MQ=255)
                                                                                                                                    ccTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTACg       >  7:233611/1‑149 (MQ=255)
                                                                                                                                      gcgaCC‑GCCCAGGCGGTTGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTACGtg     >  7:92914/4‑149 (MQ=255)
                                                                                                                                       cAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTAc        >  7:276382/1‑145 (MQ=255)
                                                                                                                                       cAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTAc        <  8:276382/145‑1 (MQ=255)
                                                                                                                                         aCC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTACGtggct  <  8:273976/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GGCCGCAGGCTTTTGTAATGCGGCCGGCAACCCACGCCTCAAACGCATCGTGCAGCGCCTGCTGCAGGATACCGCGCGGCTGATCGAAGACCTGGACATCAGCGAAGACGAGTTCTGGCACGCCGTCGATTACCTCAACC‑GCCTGGGCGGTCGCGGCGAAGCCGGGTTGCTGGTGGCGGGGCTGGGCATCGAACACTTCCTCGACCTGCTGCAGGATGCCAAGGACCAGGAGGCAGGGCGCGTTGGCGGCACCCCACGCACCATCGAAGGCCCGTTGTACGTGGCT  >  NC_002947/3587215‑3587500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑