Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,641,239 T→G 7.7% D180A (GAC→GCC)  PP_3208 ← Pdr/VanB family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,641,2390TG 7.7% 153.6 / 3.9 52D180A (GAC→GCC) PP_3208Pdr/VanB family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (20/28);  new base G (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGA  >  NC_002947/3641103‑3641382
                                                                                                                                        |                                                                                                                                                
gACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGc                                                                                                                                       <  4:214029/148‑1 (MQ=255)
   gTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAACCCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGGCCTGGGACGGCagcg                                                                                                                                   <  6:144534/149‑4 (MQ=255)
      cAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAgtgt                                                                                                                                 <  2:71351/148‑1 (MQ=255)
           ggggCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGGTGGCCAGCCGGTTGCAAGCGCGACTCGTTCTACACACTCCTTGAAACCGGCAGGGCCACAGATTTAGAGGCTTGGGCTGCCGTGCCAGGCCGGCAAGTGTtcctc                                                                                                                            <  2:108654/147‑1 (MQ=255)
               ctctGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTtc                                                                                                                               <  7:169325/141‑1 (MQ=255)
               ctctGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTtc                                                                                                                               >  8:169325/1‑141 (MQ=255)
               ctctGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAgg                                                                                                                        >  8:7229/1‑148 (MQ=255)
                    aGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCg                                                                                                                 >  8:31506/1‑149 (MQ=255)
                      gCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGt                                                                                                               >  3:42834/1‑149 (MQ=255)
                        cTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGa                                                                                                             <  8:270156/149‑1 (MQ=255)
                              aTGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCg                                                                                                       <  6:222205/149‑1 (MQ=255)
                                            gcgcCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCt                                                                                         >  1:81645/1‑149 (MQ=255)
                                            gcgcCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCt                                                                                         <  7:7229/149‑1 (MQ=255)
                                            gcgcCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCt                                                                                         >  2:302961/1‑149 (MQ=255)
                                                cGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCAt                                                                                     <  1:302961/149‑1 (MQ=255)
                                                cGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCAt                                                                                     >  7:204726/1‑149 (MQ=255)
                                                   ggTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGGGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCg                                                                                                       >  1:97102/1‑128 (MQ=255)
                                                   ggTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCg                                                                                                       <  2:97102/128‑1 (MQ=255)
                                                    gTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGaa                                                                                  <  4:137696/148‑1 (MQ=255)
                                                    gTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAAc                                                                                 >  8:207341/1‑149 (MQ=255)
                                                    gTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAAc                                                                                 >  2:192038/1‑149 (MQ=255)
                                                       ggCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTc                                                                              >  5:56730/1‑149 (MQ=255)
                                                        gCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCg                                                                             <  7:207341/149‑1 (MQ=255)
                                                        gCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCg                                                                             <  7:242683/149‑1 (MQ=255)
                                                        gCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCg                                                                             <  8:204726/149‑1 (MQ=255)
                                                        gCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCg                                                                             <  1:192038/149‑1 (MQ=255)
                                                           aGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAACCCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTa                                                                          <  8:274514/149‑1 (MQ=255)
                                                             ccGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTAtt                                                                        >  4:41991/1‑149 (MQ=255)
                                                                   gCAAGCGCGACGCGTTCGAGACACGCCATGAAACCGGGAGGGCCAGAGCAATAGAGGCTTGCGCTGCCGTCCCTGGCCGGCAAGTGTTCCTCCAGGTA‑CGCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCAt                                                                   <  2:63775/148‑1 (MQ=255)
                                                                    cAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATc                                                                  >  6:37791/1‑148 (MQ=255)
                                                                    cAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATc                                                                  <  4:42834/148‑1 (MQ=255)
                                                                     aaGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGa                                                                <  5:37791/149‑1 (MQ=255)
                                                                               cgTTCGACACACTCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGAt                                                      <  6:56730/149‑1 (MQ=255)
                                                                               cgTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGAt                                                      <  7:31506/149‑1 (MQ=255)
                                                                                    gACACCCGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCGTGCGCTGCCGTCCCAGGCCGTCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaaaa                                                  <  3:180985/148‑1 (MQ=255)
                                                                                        cacGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCAc                                             <  3:41991/149‑1 (MQ=255)
                                                                                         acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                    >  8:215313/1‑141 (MQ=255)
                                                                                         acGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATaa                                                    <  7:215313/141‑1 (MQ=255)
                                                                                         acGCCATGAAACCGGCAGGGACACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGGAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTCCTGGATAAAAGGCAcc                                            >  4:193155/1‑149 (MQ=255)
                                                                                              aTGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTc                                       >  2:20454/1‑149 (MQ=255)
                                                                                                 aaaCCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCgg                                     >  6:148092/1‑148 (MQ=255)
                                                                                                            gCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGTCCGGCAAGTGTTCCTCCAGGTA‑CCCGGCGCTGATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCCTCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGa                         <  4:62276/149‑1 (MQ=255)
                                                                                                              cacaGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGaaa                       <  2:81645/149‑1 (MQ=255)
                                                                                                                caGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGa                                                                <  5:164884/106‑1 (MQ=255)
                                                                                                                caGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGa                                                                >  6:164884/1‑106 (MQ=255)
                                                                                                                           gCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAgcgc           <  3:193155/148‑1 (MQ=255)
                                                                                                                             ttGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCaa               >  3:155537/1‑142 (MQ=255)
                                                                                                                             ttGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCaa               <  4:155537/142‑1 (MQ=255)
                                                                                                                              acacttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCacc        >  1:224225/6‑148 (MQ=255)
                                                                                                                                 cttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTg                          >  7:89270/3‑127 (MQ=255)
                                                                                                                                 cttGCCGGCCTGGGACGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTg                          <  8:89270/125‑1 (MQ=255)
                                                                                                                                     ccGTCCCAGGCCGGCAAGTGTTCCTCCAGGTATCCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCTGTATACGCAACGAGGTACTGGATAAAAGGCACCTGTTCGGCAACGCGGGTGAAAAACAGCAAGCGCAccgaccga  >  1:36637/1‑148 (MQ=255)
                                                                                                                                        |                                                                                                                                                
GACGTGCAAGGTGGGCTCTGAGGCCTGGAAATGTTCGCGGTGAAGCGCCGTGGTTGGCCAGCCGCTTGCAAGCGCGACGCGTTCGACACACGCCATGAAACCGGCAGGGCCACAGCAATAGAGGCTTGCGCTGCCGTCCCAGGCCGGCAAGTGTTCCTCCAGGTA‑CCCGGTGATCATCGGGGCGCGCAAGCCTGCATGAACCTCGGTATTCGCATCGAGGTACTGGATAAAAGGCACCTGTTCGGCATCGCGGGTGAAAAACAGCAAGCGCACCGACCGA  >  NC_002947/3641103‑3641382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑