Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,659,906 C→G 13.0% intergenic (‑41/+34) PP_3223 ← / ← PP_3224 ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,659,9060CG13.0% 140.8 / 11.0 54intergenic (‑41/+34)PP_3223/PP_3224ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base G (4/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

AGGCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAC  >  NC_002947/3659760‑3660050
                                                                                                                                                  |                                                                                                                                                
atgtgATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGTCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCa                                                                                                                                                 <  4:200349/144‑1 (MQ=255)
        ttGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCg                                                                                                                                         <  2:139576/148‑1 (MQ=255)
            gggTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACa                                                                                                                                    <  6:60881/149‑1 (MQ=255)
              atCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAgg                                                                                                                                  <  5:106396/148‑1 (MQ=255)
               tCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAgg                                                                                                                                  >  3:54905/1‑148 (MQ=255)
                 ttGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                   >  1:227745/1‑145 (MQ=255)
                 ttGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                   <  2:227745/145‑1 (MQ=255)
                   gCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAgg                                                                                                                                  >  6:106396/1‑144 (MQ=255)
                    cTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAg                                                                                                                            >  2:17399/1‑149 (MQ=255)
                       gTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTg                                                                                                                         >  4:41712/1‑149 (MQ=255)
                                 tgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagt                                                                                                                >  2:256935/1‑148 (MQ=255)
                                  gtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagt                                                                                                                >  6:52054/1‑147 (MQ=255)
                                   tCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtca                                                                                                              <  1:256935/148‑1 (MQ=255)
                                     ggccggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCt                                                                                                                      <  4:292992/138‑1 (MQ=255)
                                     ggccggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCt                                                                                                                      >  3:292992/1‑138 (MQ=255)
                                        cggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtcag                                                                                                             <  5:53605/144‑1 (MQ=255)
                                        cggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtcag                                                                                                             >  6:53605/1‑144 (MQ=255)
                                             aGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAAc                                                                                                                                     >  7:75228/1‑115 (MQ=255)
                                             aGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAAc                                                                                                                                     <  8:75228/115‑1 (MQ=255)
                                                 aGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAg                                                                                               <  1:17399/149‑1 (MQ=255)
                                                     gTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGAAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGccc                                                                                           >  7:82967/1‑149 (MQ=255)
                                                           cccAGGGCCAGGGTGCCTAGCGCTAGAAGTTGGTGCAGTGGGGGCATGGTATTTATCCTTCAATTAATGGGGCTCTCCTGTTCCGG‑CAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCt                                                                                                         <  1:156283/128‑1 (MQ=255)
                                                           cccAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGa                                                                                     >  4:290286/1‑149 (MQ=255)
                                                            ccagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGAt                                                                                    >  1:89424/1‑149 (MQ=255)
                                                                       gTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCg                                                                          >  5:15224/1‑148 (MQ=255)
                                                                       gTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCg                                                                          <  6:15224/148‑1 (MQ=255)
                                                                          ccGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAAt                                                                       <  7:240419/148‑1 (MQ=255)
                                                                           cGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCa                                                                     >  2:131676/1‑149 (MQ=255)
                                                                             aTCGCTAGCAGTTGGTGCATTGGGGGCATGGTATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGATTCAGTCCTGGTAATCTAGGCCCTCGCGATCCAACTGCCGAATAAga                                                                   <  2:99244/149‑2 (MQ=255)
                                                                                 cgAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCAt                                                               <  3:41712/149‑1 (MQ=255)
                                                                                            tGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGt                                                    >  3:303302/1‑149 (MQ=255)
                                                                                              cAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGtt                                                   <  5:52054/148‑1 (MQ=255)
                                                                                                gTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGa                                                <  1:131676/149‑1 (MQ=255)
                                                                                                 tGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGAt                                               <  3:290286/149‑1 (MQ=255)
                                                                                                      gCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATACCGTTGGATACCtt                                          >  8:118567/1‑149 (MQ=255)
                                                                                                       cATGGGATTGATCCTTCAATGAATGGGTCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTc                                         <  4:54905/149‑1 (MQ=255)
                                                                                                          gggATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTACTGGTAATCGAGGCCCTCGCGATCCAACTAACGAATCACCGCATCCCAATGCCGTTGGATACCTTccc                                       >  3:174397/1‑148 (MQ=255)
                                                                                                                             atgGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGc                   >  7:142157/1‑149 (MQ=255)
                                                                                                                                gggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCtt                 <  4:303302/148‑1 (MQ=255)
                                                                                                                                   ctctCCTGTGCCGGCCACTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGAc             <  4:216145/149‑1 (MQ=255)
                                                                                                                                    tctcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCCACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCGTCCCCCTGGCCCACGGAAACCCGCTTGGCCt            >  8:285584/1‑149 (MQ=255)
                                                                                                                                       ccTGTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCt         >  8:229921/1‑149 (MQ=255)
                                                                                                                                       ccTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCg                            <  7:107409/130‑1 (MQ=255)
                                                                                                                                       ccTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCg                            >  8:107409/1‑130 (MQ=255)
                                                                                                                                        cTGTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGc                                 >  7:135131/1‑124 (MQ=255)
                                                                                                                                        cTGTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGc                                 <  8:135131/124‑1 (MQ=255)
                                                                                                                                        cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTAGCCATCGGAAAACCGCTTGGACTGCTc        <  2:89424/149‑1 (MQ=255)
                                                                                                                                        cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCAGGTAATCGAGGCCCTCGCGATCCAACTGACGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGATAACCGCTTGGACTGCt         >  7:131800/1‑148 (MQ=255)
                                                                                                                                         ggtgccGGCCAGTTCGCCGGAACAGGCAAAAGGGGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCa       <  7:285584/145‑1 (MQ=255)
                                                                                                                                          gTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATc                             >  8:63027/1‑126 (MQ=255)
                                                                                                                                          gTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATc                             <  7:63027/126‑1 (MQ=255)
                                                                                                                                          gTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCAt      >  8:43178/1‑149 (MQ=255)
                                                                                                                                              cGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCACATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAc  >  3:26394/1‑149 (MQ=255)
                                                                                                                                              cGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCACATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAc  <  4:26394/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AGGCGATGTTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAC  >  NC_002947/3659760‑3660050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑