Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,659,909	T→C	12.9%	intergenic (‑44/+31)	PP_3223 ← / ← PP_3224	ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,659,909	0	T	C	12.9%	130.7 / 10.4	54	intergenic (‑44/+31)	PP_3223/PP_3224	ABC transporter substrate‑binding protein/class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base C (4/3);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

TTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCTTGA  >  NC_002947/3659768‑3660055                                                                                                                                              |                                                                                                                                                   ttGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCg                                                                                                                                              <  2:139576/148‑1 (MQ=255)     gggTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACa                                                                                                                                         <  6:60881/149‑1 (MQ=255)       atCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAgg                                                                                                                                       <  5:106396/148‑1 (MQ=255)        tCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAgg                                                                                                                                       >  3:54905/1‑148 (MQ=255)          ttGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                        <  2:227745/145‑1 (MQ=255)          ttGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCGAACTGGCCGGAACAg                                                                                                                                        >  1:227745/1‑145 (MQ=255)            gCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAgg                                                                                                                                       >  6:106396/1‑144 (MQ=255)             cTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAg                                                                                                                                 >  2:17399/1‑149 (MQ=255)                gTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTg                                                                                                                              >  4:41712/1‑149 (MQ=255)                          tgtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagt                                                                                                                     >  2:256935/1‑148 (MQ=255)                           gtCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagt                                                                                                                     >  6:52054/1‑147 (MQ=255)                            tCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtca                                                                                                                   <  1:256935/148‑1 (MQ=255)                              ggccggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCt                                                                                                                           <  4:292992/138‑1 (MQ=255)                              ggccggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCt                                                                                                                           >  3:292992/1‑138 (MQ=255)                                 cggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtcag                                                                                                                  >  6:53605/1‑144 (MQ=255)                                 cggcGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGagtcag                                                                                                                  <  5:53605/144‑1 (MQ=255)                                      aGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAAc                                                                                                                                          <  8:75228/115‑1 (MQ=255)                                      aGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAAc                                                                                                                                          >  7:75228/1‑115 (MQ=255)                                          aGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAg                                                                                                    <  1:17399/149‑1 (MQ=255)                                              gTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGAAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGccc                                                                                                >  7:82967/1‑149 (MQ=255)                                                    cccAGGGCCAGGGTGCCTAGCGCTAGAAGTTGGTGCAGTGGGGGCATGGTATTTATCCTTCAATTAATGGGGCTCTCCTGTTCCGG‑CAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCt                                                                                                              <  1:156283/128‑1 (MQ=255)                                                    cccAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGa                                                                                          >  4:290286/1‑149 (MQ=255)                                                     ccagggccagggTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGAt                                                                                         >  1:89424/1‑149 (MQ=255)                                                                gTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCg                                                                               >  5:15224/1‑148 (MQ=255)                                                                gTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCg                                                                               <  6:15224/148‑1 (MQ=255)                                                                   ccGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAAt                                                                            <  7:240419/148‑1 (MQ=255)                                                                    cGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCa                                                                          >  2:131676/1‑149 (MQ=255)                                                                      aTCGCTAGCAGTTGGTGCATTGGGGGCATGGTATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGATTCAGTCCTGGTAATCTAGGCCCTCGCGATCCAACTGCCGAATAAga                                                                        <  2:99244/149‑2 (MQ=255)                                                                          cgAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCAt                                                                    <  3:41712/149‑1 (MQ=255)                                                                                     tGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGt                                                         >  3:303302/1‑149 (MQ=255)                                                                                       cAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGtt                                                        <  5:52054/148‑1 (MQ=255)                                                                                         gTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGa                                                     <  1:131676/149‑1 (MQ=255)                                                                                          tGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGAt                                                    <  3:290286/149‑1 (MQ=255)                                                                                               gCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATACCGTTGGATACCtt                                               >  8:118567/1‑149 (MQ=255)                                                                                                cATGGGATTGATCCTTCAATGAATGGGTCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTc                                              <  4:54905/149‑1 (MQ=255)                                                                                                   gggATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTACTGGTAATCGAGGCCCTCGCGATCCAACTAACGAATCACCGCATCCCAATGCCGTTGGATACCTTccc                                            >  3:174397/1‑148 (MQ=255)                                                                                                                      atgGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGc                        >  7:142157/1‑149 (MQ=255)                                                                                                                         gggCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCtt                      <  4:303302/148‑1 (MQ=255)                                                                                                                            ctctCCTGTGCCGGCCACTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGAc                  <  4:216145/149‑1 (MQ=255)                                                                                                                             tctcCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCCACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCGTCCCCCTGGCCCACGGAAACCCGCTTGGCCt                 >  8:285584/1‑149 (MQ=255)                                                                                                                                ccTGTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCt              >  8:229921/1‑149 (MQ=255)                                                                                                                                ccTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCg                                 >  8:107409/1‑130 (MQ=255)                                                                                                                                ccTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCg                                 <  7:107409/130‑1 (MQ=255)                                                                                                                                 cTGTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGc                                      <  8:135131/124‑1 (MQ=255)                                                                                                                                 cTGTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGc                                      >  7:135131/1‑124 (MQ=255)                                                                                                                                 cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTAGCCATCGGAAAACCGCTTGGACTGCTc             <  2:89424/149‑1 (MQ=255)                                                                                                                                 cTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCAGGTAATCGAGGCCCTCGCGATCCAACTGACGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGATAACCGCTTGGACTGCt              >  7:131800/1‑148 (MQ=255)                                                                                                                                  ggtgccGGCCAGTTCGCCGGAACAGGCAAAAGGGGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCa            <  7:285584/145‑1 (MQ=255)                                                                                                                                   gTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATc                                  <  7:63027/126‑1 (MQ=255)                                                                                                                                   gTTCCGGCGAACTGGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATc                                  >  8:63027/1‑126 (MQ=255)                                                                                                                                   gTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCAt           >  8:43178/1‑149 (MQ=255)                                                                                                                                       cGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCACATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAc       >  3:26394/1‑149 (MQ=255)                                                                                                                                       cGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCACATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCAc       <  4:26394/149‑1 (MQ=255)                                                                                                                                             gTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGACGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCTTGa  >  8:134471/1‑148 (MQ=255)                                                                                                                                              |                                                                                                                                                   TTGAGGGTCTTGCTGGTTGAGGTGTTGTCGGCCGGCGAGCAAGCGGTGAGGCCCAGGGCCAGGGTGCCGATCGCGAGCAGTTGGTGCAGTGGGGGCATGGGATTGATCCTTCAATGAATGGGGCTCTCCTGTTCCGGCCAGTTCGCCGGAACAGGCAAAAGGTGCCTTGGAGTCAGTCCTGGTAATCGAGGCCCTCGCGATCCAACTGCCGAATCAGCGCATCCCAATGCCGTTGGATACCTTCCCCCTGGCCATCGGAAAACCGCTTGGACTGCTCATGCACCTTGA  >  NC_002947/3659768‑3660055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑