Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,692,747 G→C 9.3% S293R (AGC→AGG)  PP_3259 ← acyl‑CoA dehydrogenase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,692,7470GC 9.3% 128.8 / 4.5 43S293R (AGC→AGG) PP_3259acyl‑CoA dehydrogenase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (20/19);  new base C (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

GGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCACGGGCGCCATGCAACG  >  NC_002947/3692600‑3692877
                                                                                                                                                      |                                                                                                                                   
ggCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGc                                                                                                                                    >  2:227078/1‑149 (MQ=255)
 gCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCt                                                                                                                                   <  7:183969/149‑1 (MQ=255)
     gCAACTGGGCCAGCTGCTGGCCCAGCATGGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTAt                                                                                                                                >  1:171547/1‑148 (MQ=255)
       aaCTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGAc                                                                                                                             >  4:190109/1‑149 (MQ=255)
              ccAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGctc                                                                                                                       >  1:144443/1‑148 (MQ=255)
                aGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTAc                                                                                                                    <  2:171547/149‑1 (MQ=255)
                 gctgctGGCCCAACATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACg                                                                                                                   <  4:118213/149‑1 (MQ=255)
                  ctgctgGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACgg                                                                                                                  >  4:294412/1‑149 (MQ=255)
                   tgctgGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GCCACGTCGGACGTGCCttg                                                                                                                                 >  6:204043/1‑132 (MQ=255)
                   tgctgGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GCCACGTCGGACGTGCCttg                                                                                                                                 <  5:204043/133‑2 (MQ=255)
                        gCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGtt                                                                                                            >  1:204838/1‑149 (MQ=255)
                                  gCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAATGGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTgg                                                                                                    <  1:227078/148‑1 (MQ=255)
                                      gATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCgg                                                                                              <  6:269142/149‑1 (MQ=255)
                                      gATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCgg                                                                                              <  2:204838/149‑1 (MQ=255)
                                              tCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑gcggc                                                                                      >  2:218972/1‑148 (MQ=255)
                                              tCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑gcggc                                                                                      >  6:87742/1‑148 (MQ=255)
                                                         ctGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAAc                                                                          >  5:267579/1‑149 (MQ=255)
                                                            aCGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACt                                                                         <  6:267579/147‑1 (MQ=255)
                                                            aCGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTg                                                                        <  3:294412/148‑1 (MQ=255)
                                                             cGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGCTCCCATTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTg                                                                        <  1:102425/149‑1 (MQ=255)
                                                             cGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGc                                                                      <  6:83251/149‑1 (MQ=255)
                                                              gCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGATGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGc                                                                      >  3:237142/1‑148 (MQ=255)
                                                                aGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATc                                                                   <  3:190109/149‑1 (MQ=255)
                                                                                  gcgcTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTgatc                                                 >  6:195864/1‑149 (MQ=255)
                                                                                        aGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGTGGCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTgatcgatc                                             <  1:218972/148‑1 (MQ=255)
                                                                                        aGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTgatcgatc                                             <  5:87742/147‑1 (MQ=255)
                                                                                         gCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAg                                          <  1:149600/149‑1 (MQ=255)
                                                                                                         ctgctgCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACg                            >  2:165182/1‑149 (MQ=255)
                                                                                                         ctgctgCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACg                            <  2:144443/149‑1 (MQ=255)
                                                                                                                gACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGAc                     >  5:280065/1‑149 (MQ=255)
                                                                                                                  cAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGc                   <  5:195864/149‑1 (MQ=255)
                                                                                                                   aGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCa                  >  3:1334/1‑149 (MQ=255)
                                                                                                                    gAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGCCCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGCAACTGGCATCCACACCGGGCTGTTGATCGAGCCAGGCAGCCCATTCACGCAGTGACGCAc                 >  7:253021/1‑149 (MQ=255)
                                                                                                                    gAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTGTACGGCCTGTGCTACCTGGGCGCGGGT‑GCGGCGAGCTTCGAAACTGGCGTCCACACCGGGCTGGTGATCGGGCCAGGGAGCGCATGCACGCAGTGACGCAc                 >  2:271864/1‑149 (MQ=255)
                                                                                                                          cGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCACGGgcgc           <  1:165182/149‑1 (MQ=255)
                                                                                                                             ggCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCTGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCAAGGCAGCACATTCACGCAGTGACGCACGGGCGCAAt        >  5:45084/1‑149 (MQ=255)
                                                                                                                               gtcataa‑GCCACGTCGGACGTGCCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCACGGgcgc           <  6:313044/139‑1 (MQ=255)
                                                                                                                               gtcataa‑GCCACGTCGGACGTGCCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCACGGgcgc           >  5:313044/6‑144 (MQ=255)
                                                                                                                                ccgcAA‑GGCACGTCCGACGTGGCGTATGACCTGCTCTACGGCCTGGTGTACCTGGGCGCGGGT‑GGGGAGGGCGGCGAATCTGGCTGGCGCACCGGGGTGGTGGAGGATGGAGGGAGCACATTCGCGGAGTGCCGGACGTGCGCCAtctg     >  8:245538/4‑146 (MQ=255)
                                                                                                                                 cccAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCACGGGCGCCATGCaa    >  1:23172/1‑149 (MQ=255)
                                                                                                                                   cAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACAt                                 >  4:143781/1‑118 (MQ=255)
                                                                                                                                   cAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACAt                                 <  3:143781/118‑1 (MQ=255)
                                                                                                                                   cAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCACGGGCGCCATGCAACg  <  2:23172/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                   
GGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATG‑‑ACTGCTGCGACAGAACGGCGTGGCCCCCAA‑GGCACGTCCGACGTGGCTTATGACCTGCTCTACGGCCTGTTCTACCTGGGCGCGGGT‑GCGGCGAACTTCGAAACTGGCATCCACACCGGGCTGTTGATCGATCCAGGCAGCACATTCACGCAGTGACGCACGGGCGCCATGCAACG  >  NC_002947/3692600‑3692877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑