Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,697,084 C→G 10.3% W135C (TGG→TGC)  PP_5595 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,697,0840CG10.3% 170.5 / 10.2 58W135C (TGG→TGC) PP_5595hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base G (3/3);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

CGTTGGATGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCGGGGGTCAGTG  >  NC_002947/3696937‑3697232
                                                                                                                                                   |                                                                                                                                                    
cGTTGGATGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGc                                                                                                                                                      <  6:201573/148‑1 (MQ=255)
      aTGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTCTGCCCTAGTGGGTCTGCCAGAccc                                                                                                                                               >  1:43761/1‑149 (MQ=255)
      aTGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTCTGCCCTAGTGGGTCTGCCAGAccc                                                                                                                                               >  1:48112/1‑149 (MQ=255)
       tGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAccca                                                                                                                                              >  7:11329/1‑148 (MQ=255)
        gAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCg                                                                                                                                             >  4:205813/1‑149 (MQ=255)
          aaCCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAcccacta                                                                                                                                           <  8:11329/149‑5 (MQ=255)
           aCCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAcccactag                                                                                                                                          <  2:74820/149‑6 (MQ=255)
           aCCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGGCAGAcccactag                                                                                                                                          <  3:271405/149‑6 (MQ=255)
             cTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTAtt                                                                                                                                        <  6:277511/149‑1 (MQ=255)
                cccAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTAttt                                                                                                                                       <  4:236241/147‑1 (MQ=255)
                 ccAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCg                                                                                                                                     <  5:259415/148‑1 (MQ=255)
                 ccAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGc                                                                                                                                    >  7:83261/1‑149 (MQ=255)
                  cAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCt                                                                                                                                   >  7:174470/1‑149 (MQ=255)
                           aTCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCAc                                                                                                                          >  2:296936/1‑149 (MQ=255)
                               gATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACg                                                                                                                      >  7:78237/1‑149 (MQ=255)
                                aTCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACgg                                                                                                                     >  3:264091/1‑149 (MQ=255)
                                        cTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGcc                                                                                                             <  4:130210/149‑1 (MQ=255)
                                           aaGCAGAATAGGCGGGCTGCCCTTGCAGGCCATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAg                                                                                                          >  1:26278/1‑149 (MQ=255)
                                             gCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGc                                                                                                        >  5:194592/1‑149 (MQ=255)
                                                gAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCg                                                                                                     >  1:100933/1‑149 (MQ=255)
                                                 aaTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTCTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCgg                                                                                                    <  2:48112/149‑1 (MQ=255)
                                                 aaTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTCTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCgg                                                                                                    <  2:43761/149‑1 (MQ=255)
                                                  aTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCggg                                                                                                   <  3:205813/149‑1 (MQ=255)
                                                  aTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCggg                                                                                                   <  6:173489/149‑1 (MQ=255)
                                                  aTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCggg                                                                                                   <  6:194592/149‑1 (MQ=255)
                                                  aTAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCAAATTCTGGGAGCGGGCGTGCGCTGGCTCCCAGCCTATTGCCCTAGTGGGTCTGCCTGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGTGTCTCgg                                                                                                    <  5:58689/148‑1 (MQ=255)
                                                      gCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCTGCGTa                                                                                                >  8:291005/1‑148 (MQ=255)
                                                       cGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATc                                                                                              <  1:296936/149‑1 (MQ=255)
                                                        gggCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCg                                                                                             <  8:174470/149‑1 (MQ=255)
                                                          gCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGACTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGt                                                                                            >  3:299397/1‑148 (MQ=255)
                                                           cTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTaa                                                                                          <  8:83261/149‑1 (MQ=255)
                                                             gCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATa                                                                                        >  3:211192/1‑149 (MQ=255)
                                                                    cAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGACCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTAGGGGTATCGTAATAGTAAGta                                                                                 >  1:291703/1‑148 (MQ=255)
                                                                    cAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAACGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAg                                                                                   <  2:291703/147‑1 (MQ=255)
                                                                        cGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCa                                                                             <  2:299115/149‑1 (MQ=255)
                                                                         gATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCAc                                                                            >  8:229330/1‑149 (MQ=255)
                                                                                        tGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTgg                                                              <  4:211192/148‑1 (MQ=255)
                                                                                        tGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTg                                                               <  4:264091/147‑1 (MQ=255)
                                                                                        tGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTg                                                               <  8:78237/147‑1 (MQ=255)
                                                                                        tGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTg                                                               <  2:100933/147‑1 (MQ=255)
                                                                                         gATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCTCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTggg                                                             >  1:15921/1‑148 (MQ=255)
                                                                                              aaTTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGc                                                       >  2:23096/1‑149 (MQ=255)
                                                                                                   tcggcgCGGGCGTCCGCGCGCTCCCAGCCTTTAGCCCAACTGCCTCTGCCAGACCCCGTTATTTCGCTCCCAGTCACAACCGGCCTCCCCCAGGCTCGGGGTATCGTACTAGTAAGTGAACACCCGCGCATCCTTCGGCAACGCCgg                                                    <  8:218293/142‑1 (MQ=255)
                                                                                                   tGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCt                                                  >  4:217131/1‑149 (MQ=255)
                                                                                                          gggCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGcc                                           <  4:299397/149‑1 (MQ=255)
                                                                                                              ttGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCAcc                                        >  8:169159/1‑148 (MQ=255)
                                                                                                                          ccAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTgg                             <  2:26278/147‑1 (MQ=255)
                                                                                                                              ccTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTgg                       >  5:299881/1‑149 (MQ=255)
                                                                                                                                 tttGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGcc                     >  1:279669/1‑148 (MQ=255)
                                                                                                                                    gCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAAt                  <  6:299881/148‑1 (MQ=255)
                                                                                                                                      ccTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAAt                  >  2:213148/1‑146 (MQ=255)
                                                                                                                                      ccTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAAt                  <  1:213148/146‑1 (MQ=255)
                                                                                                                                      ccTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGcc               >  2:48265/1‑149 (MQ=255)
                                                                                                                                         acggggTCTGGCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCg            >  7:156914/4‑149 (MQ=255)
                                                                                                                                          gTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCgg           <  1:48265/149‑1 (MQ=255)
                                                                                                                                           tGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCggg          >  7:49188/1‑149 (MQ=255)
                                                                                                                                           ggggTCTGGCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCggg          >  2:151430/2‑149 (MQ=255)
                                                                                                                                             ggTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGGATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCggggg        >  7:162775/1‑149 (MQ=255)
                                                                                                                                               tCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCGGGGGt       >  3:279235/1‑148 (MQ=255)
                                                                                                                                                   ccAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCGGGGGTCAGTg  <  1:23096/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTTGGATGAAACCTTCCCAGCGAGTGATCCGATCTCGCCCTGAAGCAGAATAGGCGGGCTGCCCTTGCAGGCGATCGCCCCGCCCTTTGATCTAATTCTGGGAGCGGGCTTGCGCTGGCTCCCAGCCTTTTGCCCTAGTGGGTCTGCCAGACCCCGTTATTTCGCTCACAGTCACTACGGGCCTGGCCGAGGCTCGGGGTATCGTAATAGTAAGTGATCACCCGCGCATCCTTGGGCAACGCCGGCTTCTCGCCACCTATTGCCTTGGGATTGGCCAATGCCTCGGGGGTCAGTG  >  NC_002947/3696937‑3697232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑