Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,919,380 T→C 10.2% intergenic (+33/‑29) mexB → / → mobA multidrug resistance protein MexB/molybdenum cofactor guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,919,3800TC10.2% 98.3 / 3.2 39intergenic (+33/‑29)mexB/mobAmultidrug resistance protein MexB/molybdenum cofactor guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (18/17);  new base C (2/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

TCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGTTGATTGCAC  >  NC_002947/3919232‑3919523
                                                                                                                                                    |                                                                                                                                               
tCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTtct                                                                                                                                                 >  2:7635/1‑149 (MQ=255)
ccAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTtct                                                                                                                                                 >  5:142473/2‑149 (MQ=255)
  aGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTtctc                                                                                                                                                <  4:79842/148‑1 (MQ=255)
              gCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGc                                                                                                                                    <  8:24747/148‑1 (MQ=255)
               cGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGccc                                                                                                                                  <  6:142473/149‑1 (MQ=255)
                     tgtTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCCGGGCAGTCATTGCCGAGAAcc                                                                                                                            <  6:193313/149‑1 (MQ=255)
                                gtgtTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGc                                                                                                                 >  6:284083/1‑149 (MQ=255)
                                gtgtTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGc                                                                                                                 >  4:223740/1‑149 (MQ=255)
                                     cttcGTGGTGGTGATGCGCCTGTCAGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGGGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGCGGGTTCTCGGCAATGACTGCCCGGACCCCTTTGTCATGCCTgcc                                                                                                            >  8:231831/1‑147 (MQ=255)
                                       tcGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCCGGGCAGTCATTGCCGAGAACCCt                                                                                                                          >  4:22205/1‑133 (MQ=255)
                                       tcGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCCGGGCAGTCATTGCCGAGAACCCt                                                                                                                          <  3:22205/133‑1 (MQ=255)
                                        cGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGAc                                                                                                            <  3:181547/146‑1 (MQ=255)
                                        cGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGc                                                                                                          <  3:223740/148‑1 (MQ=255)
                                          tggtggtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCAt                                                                                                                   <  1:151849/137‑1 (MQ=255)
                                          tggtggtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCAt                                                                                                                   >  2:151849/1‑137 (MQ=255)
                                           ggtggtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTc                                                                                                      >  7:186328/1‑149 (MQ=255)
                                                    gcgcCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGc                                                                                             >  2:183605/1‑149 (MQ=255)
                                                      gcCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTc                                                                                           >  3:18319/1‑149 (MQ=255)
                                                       ccTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTc                                                                                           >  2:191846/1‑148 (MQ=255)
                                                             cGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCt                                                                                     >  3:217656/1‑148 (MQ=255)
                                                              gggCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCa                                                                                   >  7:88217/1‑149 (MQ=255)
                                                                 cGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATtc                                                                                <  8:186328/149‑1 (MQ=255)
                                                                     gCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTc                                                                                    <  6:4792/141‑1 (MQ=255)
                                                                     gCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTc                                                                                    >  5:4792/1‑141 (MQ=255)
                                                                                     cGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAg                                                            <  7:212040/149‑1 (MQ=255)
                                                                                           gCACCCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGCCTGCCGGGCCCCCTTTTTCATGCCTGACGCACTCCCCCCCTGCACCATCCTCATTCTCCCCGGTGGCCGCCGACAGCGCATg                                                      >  5:95273/1‑149 (MQ=255)
                                                                                           gCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATg                                                      >  4:176997/1‑149 (MQ=255)
                                                                                             aaCCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATggg                                                    <  4:18319/149‑1 (MQ=255)
                                                                                             aaCCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATggg                                                    >  2:261049/1‑149 (MQ=255)
                                                                                                     ccccGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGt                                             <  1:261049/148‑1 (MQ=255)
                                                                                                         gcgAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACaa                                        <  5:284083/149‑1 (MQ=255)
                                                                                                          cgAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAg                                       <  1:191846/149‑1 (MQ=255)
                                                                                                             aaGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGc                                    <  3:176997/149‑1 (MQ=255)
                                                                                                                cTGACTGGAAAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACACTTTTTCATGACTGACGCACTCCCCCCCTGCTCCATCCACATACACGCCGGTGGCCGCGGCCAGCGCATGCGCGGCCGTGACAAGGGCCtg                                 >  2:152530/1‑149 (MQ=255)
                                                                                                                   aCTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCtggtg                              <  8:88217/149‑1 (MQ=255)
                                                                                                                   aCTCGACAAGCGCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTGATTCTCGCCGGTGGCAGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCtggt                               <  1:152530/148‑1 (MQ=255)
                                                                                                                     tGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTg                                            <  2:60944/133‑1 (MQ=255)
                                                                                                                     tGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTg                                            >  1:60944/1‑133 (MQ=255)
                                                                                                                            gCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCACCCTGCTCCATCCTCATTATCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCAGTGACAAGCGCCTGTTGGACTGGc                       >  6:205149/1‑147 (MQ=255)
                                                                                                                               ccGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGc                  >  6:171807/1‑149 (MQ=255)
                                                                                                                                        gaaaaaGGGTTCCGGGCAGTCATTGCCGAGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGtt          >  4:254742/6‑148 (MQ=38)
                                                                                                                                               ggTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGTTGATTGcac  <  2:105486/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TCAGCGCCACCCTGGCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGTTGATTGCAC  >  NC_002947/3919232‑3919523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑