Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,919,390 C→T 9.5% intergenic (+43/‑19) mexB → / → mobA multidrug resistance protein MexB/molybdenum cofactor guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,919,3900CT 9.5% 111.3 / 2.8 42intergenic (+43/‑19)mexB/mobAmultidrug resistance protein MexB/molybdenum cofactor guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (20/18);  new base T (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

GCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGTTGATTGCACATGTTCATCGAGC  >  NC_002947/3919246‑3919536
                                                                                                                                                |                                                                                                                                                  
gCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGc                                                                                                                                                 <  8:24747/148‑1 (MQ=255)
 cGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGccc                                                                                                                                               <  6:142473/149‑1 (MQ=255)
       tgtTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCCGGGCAGTCATTGCCGAGAAcc                                                                                                                                         <  6:193313/149‑1 (MQ=255)
                  gtgtTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGc                                                                                                                              >  6:284083/1‑149 (MQ=255)
                  gtgtTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGc                                                                                                                              >  4:223740/1‑149 (MQ=255)
                       cttcGTGGTGGTGATGCGCCTGTCAGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGGGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGCGGGTTCTCGGCAATGACTGCCCGGACCCCTTTGTCATGCCTgcc                                                                                                                         >  8:231831/1‑147 (MQ=255)
                         tcGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCCGGGCAGTCATTGCCGAGAACCCt                                                                                                                                       >  4:22205/1‑133 (MQ=255)
                         tcGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCCGGGCAGTCATTGCCGAGAACCCt                                                                                                                                       <  3:22205/133‑1 (MQ=255)
                          cGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGAc                                                                                                                         <  3:181547/146‑1 (MQ=255)
                          cGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGc                                                                                                                       <  3:223740/148‑1 (MQ=255)
                            tggtggtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCAt                                                                                                                                <  1:151849/137‑1 (MQ=255)
                            tggtggtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCAt                                                                                                                                >  2:151849/1‑137 (MQ=255)
                             ggtggtgATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTc                                                                                                                   >  7:186328/1‑149 (MQ=255)
                                      gcgcCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGc                                                                                                          >  2:183605/1‑149 (MQ=255)
                                        gcCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTc                                                                                                        >  3:18319/1‑149 (MQ=255)
                                         ccTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTc                                                                                                        >  2:191846/1‑148 (MQ=255)
                                               cGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCt                                                                                                  >  3:217656/1‑148 (MQ=255)
                                                gggCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCa                                                                                                >  7:88217/1‑149 (MQ=255)
                                                   cGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATtc                                                                                             <  8:186328/149‑1 (MQ=255)
                                                       gCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTc                                                                                                 <  6:4792/141‑1 (MQ=255)
                                                       gCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTc                                                                                                 >  5:4792/1‑141 (MQ=255)
                                                                       cGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAg                                                                         <  7:212040/149‑1 (MQ=255)
                                                                             gCACCCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGCCTGCCGGGCCCCCTTTTTCATGCCTGACGCACTCCCCCCCTGCACCATCCTCATTCTCCCCGGTGGCCGCCGACAGCGCATg                                                                   >  5:95273/1‑149 (MQ=255)
                                                                             gCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATg                                                                   >  4:176997/1‑149 (MQ=255)
                                                                               aaCCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATggg                                                                 <  4:18319/149‑1 (MQ=255)
                                                                               aaCCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATggg                                                                 >  2:261049/1‑149 (MQ=255)
                                                                                       ccccGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGt                                                          <  1:261049/148‑1 (MQ=255)
                                                                                           gcgAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACaa                                                     <  5:284083/149‑1 (MQ=255)
                                                                                            cgAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAg                                                    <  1:191846/149‑1 (MQ=255)
                                                                                               aaGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGc                                                 <  3:176997/149‑1 (MQ=255)
                                                                                                  cTGACTGGAAAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACACTTTTTCATGACTGACGCACTCCCCCCCTGCTCCATCCACATACACGCCGGTGGCCGCGGCCAGCGCATGCGCGGCCGTGACAAGGGCCtg                                              >  2:152530/1‑149 (MQ=255)
                                                                                                     aCTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCtggtg                                           <  8:88217/149‑1 (MQ=255)
                                                                                                     aCTCGACAAGCGCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTGATTCTCGCCGGTGGCAGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCtggt                                            <  1:152530/148‑1 (MQ=255)
                                                                                                       tGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTg                                                         <  2:60944/133‑1 (MQ=255)
                                                                                                       tGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTg                                                         >  1:60944/1‑133 (MQ=255)
                                                                                                              gCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCACCCTGCTCCATCCTCATTATCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCAGTGACAAGCGCCTGTTGGACTGGc                                    >  6:205149/1‑147 (MQ=255)
                                                                                                                 ccGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGc                               >  6:171807/1‑149 (MQ=255)
                                                                                                                          gaaaaaGGGTTCCGGGCAGTCATTGCCGAGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGtt                       >  4:254742/6‑148 (MQ=38)
                                                                                                                                 ggTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGTTGATTGcac               <  2:105486/149‑1 (MQ=255)
                                                                                                                                       cGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGcc                          <  1:297011/132‑1 (MQ=255)
                                                                                                                                       cGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTATCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGcc                          >  2:297011/1‑132 (MQ=255)
                                                                                                                                              gACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCGTGGTGGACTGGCAAGGCGAGCCGTTGATTGCACATGTTCATCGAGc  >  6:254871/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCGGTGGTGTTCGTGCCGGTGTTCTTCGTGGTGGTGATGCGCCTGTCCGGGCGACGCCAGGCGCATGACAGCGACGGGCAACCCGTGCCCCGCGAAAGCTGACTGGACAAGCCCCGCCTGAAATGGGAGGGTTCTCGGCAATGACTGCCCGGAACCCTTTTTCATGCCTGACGCACTCCCCCCCTGCTCCATCCTCATTCTCGCCGGTGGCCGCGGCCAGCGCATGGGCGGCCGTGACAAGGGCCTGGTGGACTGGCAAGGCGAGCCGTTGATTGCACATGTTCATCGAGC  >  NC_002947/3919246‑3919536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑