Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 297,352 C→A 7.2% E218* (GAA→TAA) ‡ gshA ← glutamate‑‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947297,3520CA 7.2% 203.9 / 6.1 70E218* (GAA→TAA) ‡gshAglutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (34/31);  new base A (3/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

CCCACGGCCTTGCGCAGGCTGTCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCATTTTCCGTAC  >  NC_002947/297204‑297498
                                                                                                                                                    |                                                                                                                                                  
cccACGGCCTTGCGCAGGCTGTCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTc                                                                                                                                                    <  7:238607/149‑1 (MQ=255)
 ccACGGCCTTGCGCAGGCTGTCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCCCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTc                                                                                                                                                    >  6:229230/1‑148 (MQ=255)
      gCCTTGCGCAGGCTGGCGGTGTAGCTGGCCAGGGTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTg                                                                                                                                              <  8:52147/149‑1 (MQ=255)
              cAGGCTGTCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGt                                                                                                                                            <  4:222859/143‑1 (MQ=255)
              cAGGCTGTCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGt                                                                                                                                            >  3:222859/1‑143 (MQ=255)
               aGGCTGTCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTgcgggcg                                                                                                                                     <  6:246250/149‑1 (MQ=255)
               aGGCTGTCGGTGGAGCTGGCCAGGTTGTGGTAGCAGGGCGTACGGCCTGCCTGGGCGTTGCGCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATACGGCAGGAACAGGGTTGCGGCCTCCAGCTCTTCGAGCTGGTgcgggc                                                                                                                                      <  1:235742/148‑1 (MQ=255)
                     tCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCAc                                                                                                                                <  6:248326/148‑1 (MQ=255)
                         tgtAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGTCACGCAgg                                                                                                                           <  7:250407/149‑1 (MQ=255)
                         tgtAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAg                                                                                                                            >  4:256012/1‑148 (MQ=255)
                          gtAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAgg                                                                                                                           >  5:304143/1‑148 (MQ=255)
                           tAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGa                                                                                                                          <  6:304143/148‑1 (MQ=255)
                             ggttggcAGGTTGTTGTTGCAGGGCGTATGGGCGGCCTGGGCGTTTCTCTGTTAACCCAGGTCGCTCATGGGCAGGCTGGTGGCATAAGGCAGTAACTGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAg                                                                                                                        <  8:203110/142‑1 (MQ=255)
                                 gCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCttt                                                                                                                   <  5:233481/149‑1 (MQ=255)
                                 gCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCttt                                                                                                                   >  4:49079/1‑149 (MQ=255)
                                      gttgttGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTc                                                                                                                >  5:258707/1‑147 (MQ=255)
                                       ttgttgTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATTCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAg                                                                                                             <  6:135123/149‑1 (MQ=255)
                                         gttgtAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCGAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAggg                                                                                                           >  1:217214/1‑149 (MQ=255)
                                         gttgtAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAgg                                                                                                            <  1:287168/148‑1 (MQ=255)
                                                 aGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAg                                                                                                   <  2:116384/149‑1 (MQ=255)
                                                   ggCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGgc                                                                                                 >  1:114048/1‑149 (MQ=255)
                                                     cGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGgcgc                                                                                               >  5:197398/1‑149 (MQ=255)
                                                           gccggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCGCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGac                                                                                           >  4:13715/1‑146 (MQ=255)
                                                            ccggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCg                                                                                                        >  1:226497/1‑133 (MQ=255)
                                                            ccggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCg                                                                                                        <  2:226497/133‑1 (MQ=255)
                                                             cggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAgg                                                                                       <  6:197398/149‑1 (MQ=255)
                                                             cggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAgg                                                                                       <  6:258707/149‑1 (MQ=255)
                                                             cggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAgg                                                                                       >  6:275488/1‑149 (MQ=255)
                                                             cggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAgg                                                                                       >  3:29600/1‑149 (MQ=255)
                                                              ggccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGt                                                                                      <  4:29600/149‑1 (MQ=255)
                                                               gccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTa                                                                                     <  7:147008/149‑1 (MQ=255)
                                                                ccTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTa                                                                                     >  7:191182/1‑148 (MQ=255)
                                                                  tGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACAt                                                                                  >  5:148298/1‑149 (MQ=255)
                                                                    ggCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAgg                                                                                       >  6:40524/1‑142 (MQ=255)
                                                                    ggCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAgg                                                                                       <  5:40524/142‑1 (MQ=255)
                                                                    ggCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATca                                                                                >  7:92084/1‑149 (MQ=255)
                                                                           ctctGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGcagcc                                                                          <  6:148298/148‑1 (MQ=255)
                                                                              tGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGcagccag                                                                        <  3:256012/147‑1 (MQ=255)
                                                                                gTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTg                                                                     >  6:217605/1‑148 (MQ=255)
                                                                                     ccAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAgcgg                                                               >  2:119823/1‑149 (MQ=255)
                                                                                         gTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGATCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGcagccag                                                                        <  7:276170/136‑1 (MQ=255)
                                                                                         gTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGcagccag                                                                        >  8:276170/1‑136 (MQ=255)
                                                                                                aTGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTa                                                      <  3:49079/147‑1 (MQ=255)
                                                                                                   cgcAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGTCCTGCGAGGCGCCGATCAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAAt                                                 <  5:217605/149‑1 (MQ=255)
                                                                                                    gcAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAAt                                                 >  1:900/1‑148 (MQ=255)
                                                                                                     cAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGt                                                        >  7:107216/1‑140 (MQ=255)
                                                                                                     cAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCa                                               <  1:119823/149‑1 (MQ=255)
                                                                                                         cTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGCGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAgcgc                                           >  6:2865/1‑149 (MQ=255)
                                                                                                          tggtggCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAgcgc                                           >  6:87092/1‑148 (MQ=255)
                                                                                                            gtggCATAAGGCAGGAACAGGGTTTCTGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGa                                         <  5:229230/148‑1 (MQ=255)
                                                                                                            gtggCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGAt                                        <  2:114048/149‑1 (MQ=255)
                                                                                                                cATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAg                                    >  6:262879/1‑149 (MQ=255)
                                                                                                                      gCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCcgcc                              >  5:66229/1‑147 (MQ=255)
                                                                                                                          gAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACa                                                                                         <  8:245681/86‑1 (MQ=255)
                                                                                                                          gAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACa                                                                                         >  7:245681/1‑86 (MQ=255)
                                                                                                                          gAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACt                          <  2:217214/149‑1 (MQ=255)
                                                                                                                          gAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACt                          <  2:900/149‑1 (MQ=255)
                                                                                                                           aaCAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTg                         >  4:95437/1‑149 (MQ=255)
                                                                                                                              aGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTgg                        >  1:53533/1‑147 (MQ=255)
                                                                                                                                      ggCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGt              >  2:248975/1‑149 (MQ=255)
                                                                                                                                       gCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTAc                                                     <  5:149074/109‑1 (MQ=255)
                                                                                                                                       gCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTAc                                                     >  6:149074/1‑109 (MQ=255)
                                                                                                                                        gcaccAGCTCGAAGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCa            >  3:54347/4‑149 (MQ=255)
                                                                                                                                        cATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCa            >  5:4293/1‑149 (MQ=255)
                                                                                                                                         caccAGCTCGAAGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACa                                                                                   <  3:260252/75‑1 (MQ=255)
                                                                                                                                         caccAGCTCGAAGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACa                                                                                   >  4:260252/3‑77 (MQ=255)
                                                                                                                                         caccAGCTCGAAGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAgg                                   >  4:13897/3‑125 (MQ=255)
                                                                                                                                         caccAGCTCGAAGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAgg                                   <  3:13897/123‑1 (MQ=255)
                                                                                                                                             aGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCATTTTc       >  4:206216/1‑149 (MQ=255)
                                                                                                                                              gCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCAGTTTcc      >  7:2172/1‑149 (MQ=255)
                                                                                                                                                  ttCGTGCTGGTGCGTGCGGCCAGTCTGGAAGGCTTTGTCCAGGGTCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCATTTTCCGTAc  <  5:275488/149‑1 (MQ=255)
                                                                                                                                                  ttCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACCGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCATTTTCCGTAc  >  3:233204/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCCACGGCCTTGCGCAGGCTGTCGGTGTAGCTGGCCAGGTTGTTGTAGCAGGGCGTAAGGCCGGCCTGGGCGTTGCTCTGGTAACCCAGGTCGCTCATGCGCAGGCTGGTGGCATAAGGCAGGAACAGGGTTTCGGCATCCAGCTCTTCGAGCTGGTGCGGGCGGCCACGCAGGAAGCCTTTGTCCAGGGCCGGCGAGGCGCCGAACAGGTACATCAGCAGCCAGCTGTAGCGGCGGAAGTTACGAATCAGCGCGATGTAGGCCGACGACTGGTAGTCGCGGTCATTTTCCGTAC  >  NC_002947/297204‑297498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑