Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,925,611 C→T 8.5% D294N (GAT→AAT) ‡ peaA ← quinohemoprotein amine dehydrogenase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,925,6110CT 8.5% 128.4 / 2.8 47D294N (GAT→AAT) ‡peaAquinohemoprotein amine dehydrogenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (19/24);  new base T (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

GATGAACGCCGGTTGTACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGCCCTCTCCGGCCGTGACGCTCAT  >  NC_002947/3925465‑3925749
                                                                                                                                                   |                                                                                                                                          
gatgcACGCCGGTTGGACCGCCAGCTACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCATGCCGCGCTCATCTTGATCCGCTTCGAAGATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATc                                                                                                                                            <  4:261716/147‑1 (MQ=255)
  tgaACGCCGGTTGTACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCa                                                                                                                                        <  4:253780/149‑1 (MQ=255)
    aaCGCCGGTTGTACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAAc                                                                                                                                      <  2:140149/149‑1 (MQ=255)
    aaCGCCGGTTGTACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAAc                                                                                                                                      >  2:10143/1‑149 (MQ=255)
      cGCCGGTTGTACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCt                                                                                                                                    <  1:10143/149‑1 (MQ=255)
         cGGTTGTACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTca                                                                                                                                 >  3:169703/1‑149 (MQ=255)
               tACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGc                                                                                                                           >  1:143664/1‑149 (MQ=255)
                aCCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGcc                                                                                                                          >  8:293039/1‑149 (MQ=255)
                         aaCTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTc                                                                                                                 <  4:163739/149‑1 (MQ=255)
                                    gccttCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGtt                                                                                                        <  4:169703/147‑1 (MQ=255)
                                    gccttCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTg                                                                                                       >  7:115190/1‑148 (MQ=255)
                                       ttcttTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCGATGCCAACCtgcgc                                                                                                                                >  3:239906/1‑116 (MQ=255)
                                       ttcttTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCGATGCCAACCtgcgc                                                                                                                                <  4:239906/120‑5 (MQ=255)
                                        tcttTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGAACAg                                                                                                  >  2:30017/1‑149 (MQ=255)
                                             tACGGCGCTGACATCCACCCCGCGCTCATCCCGATCGGCCTCGCACATACGGCCCTTCATCTCGCCATCCAGCGCCGCGAA‑CACCTCGCCCAGGCTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATACCCGTTGTACAGGAtc                                                                                              <  8:115190/148‑2 (MQ=255)
                                                  cgcTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAg                                                                                        <  7:102422/149‑1 (MQ=255)
                                                   gcTTAAATCCAGGCCGCGATCATCGTGATCTGCTTCGAACATACGGCCTTTCATCTCGCCATCCATCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGc                                                                                       <  7:292600/149‑1 (MQ=255)
                                                     tGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCg                                                                                     >  6:294072/1‑149 (MQ=255)
                                                       aaaTCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCCGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCt                                                                                   >  1:211664/1‑149 (MQ=255)
                                                        aaTCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCt                                                                                   <  6:201982/148‑1 (MQ=255)
                                                            cAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGt                                                                              <  2:211664/149‑1 (MQ=255)
                                                             aGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGtt                                                                             >  4:125488/1‑149 (MQ=255)
                                                             aGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGtt                                                                             >  3:19035/1‑149 (MQ=255)
                                                                 cgcgCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAAc                                                                         <  1:30017/149‑1 (MQ=255)
                                                                    gcTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CAGCTGGCGCAGGTTGGCATCGCCAACCTTATCATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACgtt                                                                      <  6:219665/149‑3 (MQ=255)
                                                                     cTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGgtg                                                                     <  5:294072/149‑1 (MQ=255)
                                                                        aTCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCAGATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTa                                                                   <  7:84010/148‑1 (MQ=255)
                                                                         tCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTg                                                                                                       <  6:71054/111‑1 (MQ=255)
                                                                         tCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTg                                                                                                       >  5:71054/1‑111 (MQ=255)
                                                                         tCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTAcc                                                                 <  7:293039/149‑1 (MQ=255)
                                                                            tGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTc                                                              <  5:273242/149‑1 (MQ=255)
                                                                                      cGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAgcg                                                    >  1:267550/1‑149 (MQ=255)
                                                                                       gAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAgcgc                                                   >  3:92264/1‑149 (MQ=255)
                                                                                              cGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGa                                             <  3:125488/148‑1 (MQ=255)
                                                                                              cGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGAc                                            <  4:5620/149‑1 (MQ=255)
                                                                                                 ccTTTCATCTCGCCATCCAGCGCGGCGAAGAACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCt                                          >  1:22638/1‑149 (MQ=255)
                                                                                                          tcGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAATCGCCCTTGACCTCGACCTTg                                  >  7:210395/1‑147 (MQ=255)
                                                                                                              cATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTa                            >  6:8208/1‑149 (MQ=255)
                                                                                                                   aGCGCGGCGAA‑CACCTGGCCCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGcc                       <  2:143664/149‑1 (MQ=255)
                                                                                                                    gcgcGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGaa                                >  8:3004/1‑139 (MQ=255)
                                                                                                                    gcgcGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGaa                                <  7:3004/139‑1 (MQ=255)
                                                                                                                                acCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGCCCTCTCCGGCCGt           <  4:92264/149‑1 (MQ=255)
                                                                                                                                acCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGCCCTCTCCGGCCGt           >  6:270087/1‑149 (MQ=255)
                                                                                                                                acCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGCCCTCTCCGGCCGt           >  1:77448/1‑149 (MQ=255)
                                                                                                                                      cgcAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGCCCTCTCCGGCCGTGACGCt     <  8:210395/149‑1 (MQ=255)
                                                                                                                                        aaGGTTGGCGATGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTaa                                                       <  3:250435/96‑1 (MQ=255)
                                                                                                                                        aaGGTTGGCGATGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTaa                                                       >  4:250435/2‑97 (MQ=255)
                                                                                                                                         aGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGCCCTCTCCGGCCGTGACGCtcat  <  5:8208/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
GATGAACGCCGGTTGTACCGCCAGCAACTGCGCCTTGCCTTCTTTTACGGCGCTGAAATCCAGGCCGCGCTCATCGTGATCGGCTTCGAACATACGGCCTTTCATCTCGCCATCCAGCGCGGCGAA‑CACCTGGCGCAGGTTGGCATCGCCAACCTTCACATTGCCCCGCCATTCATAACCGTTGTACAGGATGGCCGAGCCGCTGCCGTTGAACGGTGTACCGTCGGCGTAAGCGCCCTTGACCTCGACCTTGAAGGTATCGCCCTCTCCGGCCGTGACGCTCAT  >  NC_002947/3925465‑3925749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑