Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 298,992 G→A 8.7% R712C (CGC→TGC)  yhgF ← transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947298,9920GA 8.7% 126.2 / 2.5 46R712C (CGC→TGC) yhgFtranscriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01

CTGATGCACGCCGATGTCGACGAAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATGTCGCGGTCGGTATCGGCAGCAATGC  >  NC_002947/298849‑299138
                                                                                                                                               |                                                                                                                                                  
cTGATGCACGCCGATGTCGACGAAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCgg                                                                                                                                               <  7:2282/149‑1 (MQ=255)
    ttcACGCCGATGTCGACGAAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTACGAGGATCATGCCCGGTTCCAGCTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTTGCGGTCTTGAACTCCGTACGTGGGTCGCGGCCGGGCtt                                                                                                                                           <  5:220716/147‑1 (MQ=255)
    tGCACGCCGATGTCGACGAAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCtt                                                                                                                                           <  2:301059/149‑1 (MQ=255)
          ccGATGTCGACGAAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAg                                                                                                                                     <  1:283740/149‑1 (MQ=255)
                  gacgaAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGc                                                                                                                              <  5:148856/148‑1 (MQ=255)
                  gacgaAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGc                                                                                                                              <  5:144408/148‑1 (MQ=255)
                   acgaAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGACCGGTTCAAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAg                                                                                                                            >  5:219677/1‑149 (MQ=255)
                        ggCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCggg                                                                                                                                              <  8:143660/126‑1 (MQ=255)
                        ggCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCggg                                                                                                                                              >  7:143660/1‑126 (MQ=255)
                             cGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGAAGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGt                                                                                                                   >  6:301578/1‑148 (MQ=255)
                                     gTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCCGTGACGGTTgg                                                                                                          >  3:205985/1‑149 (MQ=255)
                                                   cacGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGATGGCAGGCCGAAGGttt                                                                                            >  7:284719/1‑149 (MQ=255)
                                                    acGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGc                                                                                                                                             <  3:228093/99‑1 (MQ=255)
                                                    acGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGc                                                                                                                                             >  4:228093/1‑99 (MQ=255)
                                                    acGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTtc                                                                                           <  1:56622/149‑1 (MQ=255)
                                                         ctCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGTAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTtcgtcg                                                                                       <  1:249178/147‑1 (MQ=255)
                                                           cGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCgg                                                                                      <  1:225420/147‑1 (MQ=255)
                                                            gAGGATGATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTg                                                                                    <  1:37193/148‑1 (MQ=255)
                                                            gAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTtcgtcg                                                                                       >  2:249178/1‑145 (MQ=255)
                                                                aTCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACtt                                                                               >  7:112484/1‑149 (MQ=255)
                                                                   aTGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTTAACttttt                                                                            <  6:219677/149‑1 (MQ=255)
                                                                   aTGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACttttt                                                                            >  8:129040/1‑149 (MQ=255)
                                                                     gCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCg                                                                          >  1:39699/1‑149 (MQ=255)
                                                                      cccGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTtcgt                                                                                         >  3:81168/1‑133 (MQ=255)
                                                                      cccGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTtcgt                                                                                         <  4:81168/133‑1 (MQ=255)
                                                                         ggTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTc                                                                      >  3:136362/1‑149 (MQ=255)
                                                                             ccAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAg                                                                   <  2:39699/148‑1 (MQ=255)
                                                                                    cTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGa                                                           <  8:112484/149‑1 (MQ=255)
                                                                                       gAGGTCTTCGACGCCGTCCTTGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAg                                                          <  2:236884/147‑1 (MQ=255)
                                                                                                gACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGATGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGt                                               <  8:284719/149‑1 (MQ=255)
                                                                                                 aCGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGt                                               >  7:38264/1‑148 (MQ=255)
                                                                                                   gCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCAc                                            <  7:129040/149‑1 (MQ=255)
                                                                                                         cTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGGCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCa                                      <  5:243789/149‑1 (MQ=255)
                                                                                                           ggAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCagc                                    >  8:10453/1‑149 (MQ=255)
                                                                                                            gAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCAGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCagc                                    >  6:282548/1‑148 (MQ=255)
                                                                                                            gAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCAGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCagc                                    >  6:287005/1‑148 (MQ=255)
                                                                                                                   gCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGAt                            <  4:205985/149‑1 (MQ=255)
                                                                                                                    cGGTCTTGAACTCCGGACGTGGGTCGCAGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATg                           <  5:287005/149‑1 (MQ=255)
                                                                                                                    cGGTCTTGAACTCCGGACGTGGGTCGCAGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATg                           <  5:282548/149‑1 (MQ=255)
                                                                                                                     ggTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATGt                          >  5:260001/1‑149 (MQ=255)
                                                                                                                        cTTGAACCCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCagcga                                  <  5:49178/138‑1 (MQ=255)
                                                                                                                        cTTGAACACCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCagcga                                  >  6:49178/1‑138 (MQ=255)
                                                                                                                           gAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATGTCGcggt                    >  5:45964/1‑149 (MQ=255)
                                                                                                                                 cGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATGTCGCGGTCGGTAt              >  1:286408/1‑149 (MQ=255)
                                                                                                                                      gTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGa                                                           >  2:254047/1‑99 (MQ=255)
                                                                                                                                           tCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATGTCGCGGTCGGTATCGGCAGCAAt    >  7:96719/1‑149 (MQ=255)
                                                                                                                                             gcgGCCGGGCTTGTCCAGCTCTTGCAGGATGTCTGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGTTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGTGGATGTCGCGGTCGGTATCGGCAGCAATgc  <  5:301578/149‑1 (MQ=255)
                                                                                                                                              cgGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCagcgag                                 <  4:252671/117‑1 (MQ=255)
                                                                                                                                              cgGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCagcgag                                 >  3:252671/1‑117 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CTGATGCACGCCGATGTCGACGAAGGCACCGAAGTTGGTAACGTTGGTCACCACGCCTTCGAGGATCATGCCCGGTTCCAGGTCCTTGAGGTCTTCGACGCCGTCCTGGAAGGTGGCGGTCTTGAACTCCGGACGTGGGTCGCGGCCGGGCTTGTCCAGCTCTTGCAGGATGTCGGTGACGGTTGGCAGGCCGAAGGTTTCGTCGGTGAACTTTTTCGGGTCCAGGCGCTTGAGGAAGCTGCTGTCACCAATCAGCGAGCGGATGTCGCGGTCGGTATCGGCAGCAATGC  >  NC_002947/298849‑299138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑