| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,022,780 | C→G | 7.9% | intergenic (‑475/+193) | PP_3547 ← / ← emrB | NAD(P)‑binding oxidoreductase/multidrug transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,022,780 | 0 | C | G | 7.9% | 146.5 / 4.2 | 51 | intergenic (‑475/+193) | PP_3547/emrB | NAD(P)‑binding oxidoreductase/multidrug transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (23/23); new base G (2/2); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
CCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAG > NC_002947/4022632‑4022926 | tctcgctctcgctctcgctctcgctctcgctctcgcttcggcttttgattttgcttctaagcgcgcgatagcccaggcgcggcggattgcgacttcaggaggccgagcgcaGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATc < 8:3283‑M1/38‑1 (MQ=255) cGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTgg < 5:225598/148‑1 (MQ=255) gTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGATACCGGGAGGACCGCGCT‑GCAGGCCCC‑AACCTGGGGCCCAGCGCATGAc > 4:21829/1‑149 (MQ=255) tCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGCCCGCGCG‑GCAGCCCCC‑GTCCAGGGGCCCCGCGAGTGACg > 4:200909/1‑149 (MQ=14) aCTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAg < 2:6759/138‑1 (MQ=255) aCTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTACGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAg > 1:6759/1‑138 (MQ=255) tCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCATTAGCCCGCCCCAAAACGGGCGCCCAGCGCTTCACGGGCacc > 1:257054/1‑145 (MQ=14) cAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCGCCGGGGGTGGGCCCGGGGGGGCCGCGCG‑GCGGGCCCC‑CACCTGGGGGCCCGCGCTTGTCGGGCATCCCTGc > 2:245036/1‑149 (MQ=17) aaCGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGccc < 4:276350/149‑1 (MQ=255) cgACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑AGGATGGGGcctgca > 5:222233/1‑120 (MQ=14) cgACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑AGGATGGGGcctgca < 6:222233/124‑5 (MQ=14) ttgctttggcttttgcttctaagcgcgcggtagttcaggcgatgcagattgcgacttcaggaggccgagcgcaGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTc > 7:117613‑M1/74‑147 (MQ=255) aaaaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑AGGATGGGGcctgca < 4:384/120‑5 (MQ=14) aaaaaGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑AGGATGGGGcctgca > 3:384/1‑116 (MQ=14) aGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCtt < 3:156629/149‑1 (MQ=255) gTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc < 8:223053/149‑1 (MQ=255) gTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc < 5:262499/149‑1 (MQ=255) attttgcttctaagcgcgcgatagcccaggcgccgcggattgcgacttcaggaggccgagcgcaGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTc > 6:187883‑M1/65‑148 (MQ=255) cAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAg < 5:59190/149‑1 (MQ=255) aaaaaCGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGc > 5:276756/1‑148 (MQ=255) aaaaaCGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGc < 6:276756/148‑1 (MQ=255) aaaCGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCt < 2:160957/149‑1 (MQ=255) ctaagcgcgcgatagcccaggcgcggcggattgcgacttcaggaggccgagcgcaGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCt > 7:3283‑M1/56‑148 (MQ=255) tcatcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGt < 6:98016/114‑1 (MQ=32) tcatcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGt > 5:98016/1‑114 (MQ=32) atcCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGCCCCCGCT‑GCAGGCCCC‑CACCTGGGGGCCAGAGCTTGACGGGCATACATGCCCCTCACCACCCGTCGCAAGCCCAGCGCGCGGccgacgga > 6:244812/1‑142 (MQ=11) gCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTTCTCCCGGGAGGACCGCCCA‑GCAGGCCCC‑CACCAGGGGCCCAGCGCATGACGGGCCTCCAAGCCCGGCACCACCCATCCCAACCCCGGCGCGAGGCCTCCAGAACTCg > 2:237386/1‑148 (MQ=2) ccccTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGcctcct < 6:290042/140‑1 (MQ=34) ccccTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGcctcct > 5:290042/1‑140 (MQ=34) tCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTg > 1:199490/1‑149 (MQ=39) ttcaggaggccgagcgcaGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGt > 5:73816‑M1/19‑91 (MQ=255) ttcaggaggccgagcgcaGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGt < 6:73816‑M1/73‑1 (MQ=255) cGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTggg < 3:21829/149‑1 (MQ=40) ccTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgc < 2:257054/148‑1 (MQ=255) cTCGCTCCGGGCTTGCTCCCGGGAGGACGGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgcgc > 1:173057/1‑149 (MQ=255) cTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgcgc > 2:199253/1‑149 (MQ=255) cTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCt > 6:67989/1‑148 (MQ=255) GGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGaa > 2:173057‑M1/1‑149 (MQ=255) TTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCa < 1:298850‑M1/148‑1 (MQ=255) TTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCa < 3:108220‑M1/148‑1 (MQ=255) TTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCa < 1:303657‑M1/148‑1 (MQ=255) TTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGAGGCGCCTGGGCTATCGCGCGCTTAGAAGCa < 3:103694‑M1/148‑1 (MQ=255) GCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCt > 6:302749‑M1/1‑123 (MQ=255) cccGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATc > 2:169407/1‑148 (MQ=255) ccGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATc > 7:183666/1‑147 (MQ=255) ccGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATc < 8:183666/147‑1 (MQ=255) gaCCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCg < 2:147920/148‑1 (MQ=255) ccGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAag < 1:199253/149‑1 (MQ=255) cgcgCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagc < 1:237386/149‑1 (MQ=255) cgcgCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagc > 2:86320/1‑149 (MQ=255) cccc‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCGTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagag > 3:45242/1‑149 (MQ=255) aTCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgag < 2:226926/149‑1 (MQ=255) | CCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCT‑GCAGGCCCC‑ATCCTGGGGCCCAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAG > NC_002947/4022632‑4022926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |