Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,049,910 C→G 26.2% intergenic (+43/‑58) quiA → / → oprB‑III quinate dehydrogenase/carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,049,9100CG26.2% 66.1 / 27.9 42intergenic (+43/‑58)quiA/oprB‑IIIquinate dehydrogenase/carbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base C (11/20);  new base G (4/7);  total (15/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

CTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCA  >  NC_002947/4049762‑4050058
                                                                                                                                                    |                                                                                                                                                    
cTACGTCTCGCCGAAGACCGGCAAGCAGAACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGc                                                                                                                                                      >  1:175007/1‑149 (MQ=255)
   cGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGccc                                                                                                                                                    <  6:21446/148‑1 (MQ=255)
        cGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGt                                                                                                                                                <  1:207758/147‑1 (MQ=255)
        cGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGt                                                                                                                                                >  2:207758/1‑147 (MQ=255)
        cGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGccc                                                                                                                                                    <  6:103678/143‑1 (MQ=255)
        cGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGccc                                                                                                                                                    >  5:103678/1‑143 (MQ=255)
         gCCGAAGACCGGCAAGCAGTACGTGGTGAGCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACGATGTGATTGCTTACGCTCTGCCGGAAACCCGCGTCGCCTGCTTCGCGGGCCAGCCCCCCCCGACCAGGccccgggcgg                                                                                                                                             >  1:111092/1‑143 (MQ=255)
          ccGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCCCGCCAACAAAGccccaggatgg                                                                                                                                            >  6:201554/1‑142 (MQ=255)
            gAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGa                                                                                                                                            >  8:249005/1‑147 (MQ=255)
            gAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGa                                                                                                                                            <  7:249005/147‑1 (MQ=255)
            gAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGc                                                                                                                                          <  8:24667/149‑1 (MQ=255)
              aGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCgg                                                                                                                                        <  1:143570/149‑1 (MQ=255)
              aGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCgg                                                                                                                                        <  8:12822/149‑1 (MQ=255)
               gACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCTGCTCCTACAGGGGCCTTGTAGGAGCggg                                                                                                                                       <  3:105501/149‑1 (MQ=255)
                  cGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCggg                                                                                                                                       >  8:55283/1‑146 (MQ=255)
                  cGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTGTAGGAGCggg                                                                                                                                       <  7:55283/146‑1 (MQ=255)
                   ggCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGACAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTg                                                                                                                                   <  7:269209/149‑1 (MQ=255)
                               gtggtgATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGcc                                                                                                                       >  5:126182/1‑149 (MQ=255)
                               gtggtgATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGcc                                                                                                                       >  7:268409/1‑149 (MQ=255)
                                     aTCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCaa                                                                                                                 <  1:17419/149‑1 (MQ=255)
                                       cACGGCTGGCGGCGCCCGCCCGGCGCGTGCCGGTGGCGAGTTGGTGATGGCTTACGCGCTGCCGTAAACCCGCGTCGCCGGCGGCGCGGGCAAGCCCGCGCCTACACGGCCCCTGTCGTAGCGGGCTGGCCCGCGAATGCCCCTCaaa                                                                                                                <  2:111092/148‑1 (MQ=255)
                                           actgGCGGGCGCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCCAGCCCGCTCCGACAAGGCCCCTGTAGGGGCGGGCGTGCCCGCGAATGGCCCGCAAAGGCaa                                                                                                           >  4:290797/2‑149 (MQ=255)
                                                       cgccAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTg                                                                                                                                                 >  5:202271/1‑99 (MQ=255)
                                                       cgccAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAGGGGCCTTg                                                                                                                                                 <  6:202271/99‑1 (MQ=255)
                                                       cgccAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTg                                                                                               >  8:224678/1‑149 (MQ=255)
                                                       cgccAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTg                                                                                               >  3:262057/1‑149 (MQ=255)
                                                       cgccAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGCCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTg                                                                                               <  6:162399/149‑1 (MQ=255)
                                                         ccAGTCGACTGACCGTGGCGAATATGTTATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTGGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTa                                                                                             <  5:201554/149‑1 (MQ=255)
                                                                aCTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCAt                                                                                      <  7:224678/149‑1 (MQ=255)
                                                                    aCCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCg                                                                                   <  6:126182/148‑1 (MQ=255)
                                                                                 tgtgATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGccc                                                                                                                      <  3:239247/100‑1 (MQ=255)
                                                                                 tgtgATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGccc                                                                                                                      >  4:239247/1‑100 (MQ=255)
                                                                                  gtgATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCGAAGACAACCCGAGATTCTGTaa                                                                                            <  3:285627/125‑1 (MQ=255)
                                                                                        tCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCAcc                                                               <  2:175007/148‑1 (MQ=255)
                                                                                        tCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCAcc                                                               <  3:53038/148‑1 (MQ=255)
                                                                                              gCTCTGCCGTAAAACCGCGGCGCCTGCTTCGCGGGCAAGCCCGCTCCAACAAGGCCCCTGTAGGAGGGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCctgctg                                                         <  1:291972/148‑1 (MQ=255)
                                                                                                tctGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCctgctgctg                                                      <  4:262057/149‑1 (MQ=255)
                                                                                                  tGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTTGCATCACCCCCACCctgctgctg                                                      <  3:254353/147‑1 (MQ=255)
                                                                                                  tGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGctggc                                                    >  2:181353/1‑149 (MQ=255)
                                                                                                       tAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGctggccctgg                                               <  3:290797/149‑1 (MQ=255)
                                                                                                       tAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGctggccctgg                                               <  8:268409/149‑1 (MQ=255)
                                                                                                                        cTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGccc                                <  1:181353/147‑1 (MQ=255)
                                                                                                                                     cccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGcaccac                                     >  5:201344/1‑129 (MQ=255)
                                                                                                                                     cccGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGcaccac                                     <  6:201344/129‑1 (MQ=255)
                                                                                                                                       cGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGAtct                >  1:52061/1‑148 (MQ=255)
                                                                                                                                        gCTCCTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCa              >  6:303378/1‑149 (MQ=255)
                                                                                                                                        gCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCa              >  8:150584/1‑149 (MQ=255)
                                                                                                                                            cTACAGGGGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGAc          <  5:303378/149‑1 (MQ=255)
                                                                                                                                                    ccccTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCgcagca  <  7:29746/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTACGTCTCGCCGAAGACCGGCAAGCAGTACGTGGTGATCACTGCTGGCGGCGCCCGCCAGTCGACTGACCGTGGCGACTATGTGATTTCTTACGCTCTGCCGTAAAACCGCGTCGCCTGCTTCGCGGGCAAGCCCGCTCCTACAAGGCCCCTGTAGGAGCGGGCTTGCCCGCGAATGCCCCTCAAAGACAACCCGAGATTCTGTAATGCCATCCGCAATTCGCATCACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCA  >  NC_002947/4049762‑4050058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑