Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,071,349 C→T 8.3% R270R (CGG→CGA)  mdtB ← multidrug transporter membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,071,3490CT 8.3% 135.2 / 2.2 49R270R (CGG→CGA) mdtBmultidrug transporter membrane protein
Reads supporting (aligned to +/- strand):  ref base C (20/24);  new base T (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

CGATACATCGAGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGGCATCGAGCATCGACACCCGGGTCGGG  >  NC_002947/4071205‑4071493
                                                                                                                                                |                                                                                                                                                
cGATACATCGAGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCt                                                                                                                                              <  2:257976/149‑1 (MQ=255)
  aTACATCGAGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCg                                                                                                                                            >  8:195979/1‑149 (MQ=255)
      aTCGAGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTtct                                                                                                                                        >  6:219523/1‑149 (MQ=255)
       tCGAGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCACCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTtctc                                                                                                                                       >  1:161532/1‑149 (MQ=255)
         gAGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCg                                                                                                                                      <  6:175778/148‑1 (MQ=255)
          aGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGgc                                                                                                                                    >  8:262148/1‑149 (MQ=255)
              ccgccgGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCg                                                                                                                                >  6:11453/1‑149 (MQ=255)
                  cgGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTcga                                                                                                                            <  7:35061/149‑1 (MQ=255)
                  cgGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTcga                                                                                                                            <  7:1379/149‑1 (MQ=255)
                    gCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTtct                                                                                                                                        <  4:180815/135‑1 (MQ=255)
                    gCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTtct                                                                                                                                        >  3:180815/1‑135 (MQ=255)
                            tCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCgg                                                                                                                   <  5:219523/148‑1 (MQ=255)
                                  aTCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATc                                                                                                            <  2:289544/149‑1 (MQ=255)
                                  aTCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATc                                                                                                            <  5:11136/149‑1 (MQ=255)
                                    cGACGGCAGCAGGCCCTTGATTCGGTCGACCACTTCGATCACGTTGGCTCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATcc                                                                                                           <  7:44167/148‑1 (MQ=255)
                                     gACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCtt                                                                                                         <  3:251556/149‑1 (MQ=255)
                                     gACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCAGACGCTGGATGTTCAGCAGCAGTGCATGGTTCTCGTTCGCACAGGAGGCCAGCCGCTCGTGCTCGGAGCCGTCGACGATTTCGGCCACATCCtt                                                                                                         >  6:97527/1‑149 (MQ=255)
                                        ggCAGCAGGCCCTTGATGCGGTCGACCACATCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAg                                                                                                      >  2:215312/1‑149 (MQ=255)
                                          cagcagGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGgc                                                                                                    <  1:215312/149‑1 (MQ=255)
                                             cagGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCa                                                                                                 <  5:11453/149‑1 (MQ=255)
                                                           ggTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCCCCgttg                                                                                   >  7:66863/1‑149 (MQ=255)
                                                               gACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCTCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGttgttg                                                                                <  7:195979/148‑1 (MQ=255)
                                                                     ttCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGc                                                                           >  8:90076/1‑147 (MQ=255)
                                                                          tCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGTCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGAt                                                                     >  3:169124/1‑148 (MQ=255)
                                                                            aCGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAg                                                                  >  7:17602/1‑149 (MQ=255)
                                                                                 ggCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTg                                                              <  2:161532/148‑1 (MQ=255)
                                                                                  gcgcCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGTCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGc                                                            <  4:169124/149‑1 (MQ=255)
                                                                                   cgcCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCg                                                           <  7:262148/149‑1 (MQ=255)
                                                                                          cTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTc                                                                                                        <  8:249886/97‑1 (MQ=255)
                                                                                          cTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTActcct                                                    >  3:117806/1‑149 (MQ=255)
                                                                                          cTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTActcct                                                    <  7:90076/149‑1 (MQ=255)
                                                                                            gACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCAcc                                                                                       >  3:251780/1‑112 (MQ=255)
                                                                                            gACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCAcc                                                                                       <  4:251780/112‑1 (MQ=255)
                                                                                                   gATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGgaga                                             <  7:33449/147‑1 (MQ=255)
                                                                                                      gTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAgcg                                          >  1:6856/1‑147 (MQ=255)
                                                                                                                   gCATGGTTCTCCTTCGCCAAGGCCGCCAGACGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTgg                            >  6:307204/1‑148 (MQ=255)
                                                                                                                    cATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCgg                                                 >  1:276493/1‑126 (MQ=255)
                                                                                                                    cATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCgg                                                 <  2:276493/126‑1 (MQ=255)
                                                                                                                            tcGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCTGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGagag                                            >  4:120488/1‑123 (MQ=255)
                                                                                                                            tcGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCTGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGagag                                            <  3:120488/123‑1 (MQ=255)
                                                                                                                              gTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGc                                                            <  7:93563/105‑1 (MQ=255)
                                                                                                                              gTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGgcatcgagcatc                 <  8:17602/148‑1 (MQ=255)
                                                                                                                               ttCGCCCAGGCGGCCAGTCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAg                                                                             >  2:135605/1‑87 (MQ=255)
                                                                                                                               ttCGCCCAGGCGGCCAGTCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAg                                                                             <  1:135605/87‑1 (MQ=255)
                                                                                                                                   cccAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGGCATCGAGCATCGACAcc           >  2:90400/1‑149 (MQ=255)
                                                                                                                                   cccAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGGCATCGAGCATCGACAcc           >  1:240948/1‑149 (MQ=255)
                                                                                                                                    ccAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGGCATCGAGCATCGACAccc          >  8:148325/1‑149 (MQ=255)
                                                                                                                                            ccAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGGCATCGAGCATCGACACCCGGGTCggg  >  7:97724/1‑149 (MQ=255)
                                                                                                                                            ccAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGGCATCGAGCATCGACACCCGGGTCggg  <  8:97724/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGATACATCGAGGCCCGCCGGCAGGTTGTCGGTGATCGACGGCAGCAGGCCCTTGATGCGGTCGACCACTTCGATCACGTTGGCGCCCGGCTGACGCTGGATGTTCAGCAGCACTGCATGGTTCTCGTTCGCCCAGGCGGCCAGCCGCTCGTTCTCGGCGCCGTCGACGATTTCGGCCACATCCTTCAGGCGCAGCGGCGCACCGTTGTTGTAGGCCAGGATCAGGTTGGCGTACTCCTCGGGAGAGCGCAGCTGGTCGTTGGCATCGAGCATCGACACCCGGGTCGGG  >  NC_002947/4071205‑4071493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑