Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	310,280	A→T	5.7%	I699N (ATC→AAC)	PP_0256 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	310,280	0	A	T	5.7%	214.1 / 2.7	70	I699N (ATC→AAC)	PP_0256	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (32/34);  new base T (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

CGGGTTCACCTCCGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTGGCAACTGGCCGCTGGCGCG  >  NC_002947/310132‑310422                                                                                                                                                     |                                                                                                                                               cGGGTTCACCTCCGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCGCTGCTGGAAGCCCAGGCGgatga                                                                                                                                                <  2:280350/149‑1 (MQ=255)          cTCCGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGAGCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCt                                                                                                                                        >  5:264109/1‑148 (MQ=255)          cTCCGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCt                                                                                                                                        <  6:264109/148‑1 (MQ=255)             cGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCTCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTc                                                                                                                                      <  7:277975/147‑1 (MQ=255)             cGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTc                                                                                                                                      <  4:250160/147‑1 (MQ=255)             cGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCg                                                                                                                                     <  1:292654/148‑1 (MQ=255)              gggTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGtt                                                                                                                                   <  4:171419/149‑1 (MQ=255)                    tatGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGaa                                                                                                                             >  8:204616/1‑149 (MQ=255)                     atGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGaa                                                                                                                             >  5:148529/1‑148 (MQ=255)                      tGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGGAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGaa                                                                                                                             >  8:189261/1‑147 (MQ=255)                        ccgccgccTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGc                                                                                                                                <  6:43453/142‑1 (MQ=255)                        ccgccgccTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGc                                                                                                                                >  5:43453/1‑142 (MQ=255)                        ccgccgccTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAg                                                                                                                          >  2:53254/1‑148 (MQ=255)                        ccgccgccTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAg                                                                                                                          <  1:53254/148‑1 (MQ=255)                          gccgccTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCAc                                                                                                                       <  2:245609/149‑1 (MQ=255)                             gccTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTc                                                                                                                    >  2:171676/1‑149 (MQ=255)                                tGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTgg                                                                                                                                 <  6:182444/133‑1 (MQ=255)                                tGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTgg                                                                                                                                 >  5:182444/1‑133 (MQ=255)                                  cccGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGGAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCgcgc                                                                                                                <  7:189261/148‑1 (MQ=255)                                               tCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCacgcacgc                                                                                                   <  7:204616/148‑1 (MQ=255)                                                 gAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCAcgccgc                                                                                                >  2:219497/1‑149 (MQ=255)                                                 gAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCAcgccgc                                                                                                >  7:117358/1‑149 (MQ=255)                                                     gCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTa                                                                                            <  1:171676/149‑1 (MQ=255)                                                         gcagGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCAGGCCGCGGTAGCgg                                                                                        <  2:167307/149‑1 (MQ=255)                                                                aaaGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGgtcgtc                                                                                   >  1:19782/1‑147 (MQ=255)                                                                 aaGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCa                                                                                 <  6:148529/148‑1 (MQ=255)                                                                 aaGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCa                                                                                 <  5:221871/148‑1 (MQ=255)                                                                    ccTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGcc                                                                             >  2:6321/1‑149 (MQ=255)                                                                     cTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGTTGTCGGCTTCGTTgg                                                                                                                                 <  7:247689/96‑1 (MQ=255)                                                                     cTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGTTGTCGGCTTCGTTgg                                                                                                                                 <  7:252120/96‑1 (MQ=255)                                                                     cTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGTTGTCGGCTTCGTTgg                                                                                                                                 >  8:247689/1‑96 (MQ=255)                                                                     cTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGTTGTCGGCTTCGTTgg                                                                                                                                 >  8:252120/1‑96 (MQ=255)                                                                      ttGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCg                                                                            >  3:73489/1‑148 (MQ=255)                                                                              ggCGCACATGTTCATCCCCCCACAGCGAACCGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAgtg                                                                   <  8:117358/149‑1 (MQ=255)                                                                              ggCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAgtg                                                                   >  2:46651/1‑149 (MQ=255)                                                                               gcgcACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAgtgg                                                                  <  4:73489/149‑1 (MQ=255)                                                                                cgcACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAgtggt                                                                 <  6:7753/149‑1 (MQ=255)                                                                                cgcACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAgtggt                                                                 <  7:44723/149‑1 (MQ=255)                                                                                   acaTGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGtgtt                                                              >  3:138053/1‑149 (MQ=255)                                                                                   acaTGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGtgtt                                                              <  6:215074/149‑1 (MQ=255)                                                                                   acaTGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTAGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATa                                                                      <  4:282771/141‑1 (MQ=255)                                                                                   acaTGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTAGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATa                                                                      >  3:282771/1‑141 (MQ=255)                                                                                     aTGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAg                                                                         >  1:288827/1‑136 (MQ=255)                                                                                     aTGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAg                                                                         <  2:288827/136‑1 (MQ=255)                                                                                       gTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAgtg                                                                   >  4:210078/1‑140 (MQ=255)                                                                                       gTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAgtg                                                                   <  3:210078/140‑1 (MQ=255)                                                                                         tCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACt                                                         >  5:169209/1‑148 (MQ=255)                                                                                         tCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACt                                                         >  6:25204/1‑148 (MQ=255)                                                                                             ccccccACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCg                                                    >  1:206734/1‑149 (MQ=255)                                                                                                 ccACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGc                                                 >  5:170631/1‑148 (MQ=255)                                                                                                 ccACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGCCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCCCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGc                                                 >  2:115780/1‑148 (MQ=255)                                                                                                     aGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAgcg                                            >  3:286460/1‑149 (MQ=255)                                                                                                         aaaCGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATc                                        >  8:53800/1‑149 (MQ=255)                                                                                                         aaaCGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATc                                        >  8:144202/1‑149 (MQ=255)                                                                                                             gATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGAtt                                    <  1:46651/149‑1 (MQ=255)                                                                                                              aTGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTa                                                           >  4:208584/1‑125 (MQ=255)                                                                                                              aTGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTa                                                           <  3:208584/125‑1 (MQ=255)                                                                                                                 tCGACCTTCTGCCGCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCa                                 <  6:170631/148‑1 (MQ=255)                                                                                                                   gACCTTCTTCCCCGTCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTTGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGa                              <  2:19782/149‑1 (MQ=255)                                                                                                                   gACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCTGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATg                               <  5:122734/148‑1 (MQ=255)                                                                                                                         cTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAg                         >  1:290087/1‑148 (MQ=255)                                                                                                                         cTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGt                        >  1:235508/1‑149 (MQ=255)                                                                                                                            ccccGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCTTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTg                     <  5:25204/149‑1 (MQ=255)                                                                                                                            ccccGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGt                        <  7:144202/146‑1 (MQ=255)                                                                                                                            ccccGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTg                     >  6:206041/1‑149 (MQ=255)                                                                                                                              ccGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTgg                    >  2:242730/1‑148 (MQ=255)                                                                                                                              ccGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTgg                    >  4:68588/1‑148 (MQ=255)                                                                                                                              ccGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTGGc                   <  4:138053/149‑1 (MQ=255)                                                                                                                                ggCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTGGCaa                 <  3:215352/149‑1 (MQ=255)                                                                                                                                            aGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTGGCAACTGGCCGCTGgc     <  1:6321/149‑1 (MQ=255)                                                                                                                                               cGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTGGCAACTGGCCGCTGGcgcg  <  2:206734/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               CGGGTTCACCTCCGGGTAATATGCCGCCGCCTGCCCGGCAGGTATGTCGAAGGCCAGCAGGGTAAAGCCTTGCACCCGGCGCACATGTTCATCCCCCCACAGCGAAACGATGTCGACCTTCTGCCCCGGCTGGAAGCCCAGGCGGATGATGTCGGCTTCGTTGGCGAACAGCACCTCGCGCTGGCCACGCACGCCGCGGTAGCGGTCGTCCAGGCCGTAGATAGTGGTGTTGTACTGATCGTGCGAGCGCATCGATTGCATGATCAAGTCTGGCAACTGGCCGCTGGCGCG  >  NC_002947/310132‑310422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑