Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,196,302 T→G 10.2% intergenic (+144/+225) PP_5618 → / ← PP_5619 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,196,3020TG10.2% 133.3 / 6.6 49intergenic (+144/+225)PP_5618/PP_5619Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base G (2/3);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

CCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGC  >  NC_002947/4196166‑4196445
                                                                                                                                        |                                                                                                                                               
ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCg                                                                                                                                     >  7:167540/1‑149 (MQ=255)
  aCTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCggttca                                                                                                                                        <  2:57707/144‑5 (MQ=255)
                ctctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCAc                                                                                                                     >  5:270958/1‑149 (MQ=255)
                 tctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCAGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACg                                                                                                                    >  5:290571/1‑149 (MQ=255)
                      gcgAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGc                                                                                                                                           >  6:264094/1‑121 (MQ=255)
                      gcgAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGc                                                                                                                                           <  5:264094/121‑1 (MQ=255)
                                      cccGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCa                                                                                                                                 >  4:288791/1‑115 (MQ=255)
                                      cccGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCa                                                                                                                                 <  3:288791/115‑1 (MQ=255)
                                          aCAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGACACGCTTGCGCGCCCCACTTGCCTgaac                                                                                            >  8:265762/1‑145 (MQ=255)
                                            aaGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAg                                                                                                                                <  6:150802/110‑1 (MQ=255)
                                            aaGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAg                                                                                                                                >  5:150802/1‑110 (MQ=255)
                                            aaGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgcag                                                                                            >  3:233782/1‑146 (MQ=255)
                                                cttagcaagaTCTGCTAGGCGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAAt                                                                                     >  5:105529/8‑149 (MQ=38)
                                                  gCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAAtt                                                                                    <  7:265762/148‑1 (MQ=255)
                                                   cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCt                                                                                  <  7:260494/149‑1 (MQ=255)
                                                    aTGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTg                                                                                               >  1:145363/1‑135 (MQ=255)
                                                    aTGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTg                                                                                               <  2:145363/135‑1 (MQ=255)
                                                    aTGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCt                                                                                  >  4:279614/1‑148 (MQ=255)
                                                      gagaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGaa                                                                               >  8:287690/1‑149 (MQ=255)
                                                       agaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAAACGAGACGTCGACACCGTCGACGgctc                                                                                                                                              <  7:119419/85‑1 (MQ=37)
                                                       agaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAAACGAGACGTCGACACCGTCGACGgctc                                                                                                                                              >  8:119419/1‑85 (MQ=37)
                                                         aTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCGACTTGCCTGCAGCAGAAATTCTGAACAt                                                                            <  1:173362/149‑1 (MQ=255)
                                                                            tAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                         >  7:123804/1‑149 (MQ=255)
                                                                              aGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                         <  6:270958/147‑1 (MQ=255)
                                                                              aGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                         <  6:144042/147‑1 (MQ=255)
                                                                                     cGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATtgct                                                 <  8:167540/148‑1 (MQ=255)
                                                                                          ccTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                           >  4:270300/1‑149 (MQ=255)
                                                                                            tATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                         >  5:213191/1‑149 (MQ=255)
                                                                                              tGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                         <  6:290571/147‑1 (MQ=255)
                                                                                                ggCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTa                                      <  7:287690/148‑1 (MQ=255)
                                                                                                   ccGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGAc                                  >  7:75756/1‑149 (MQ=255)
                                                                                                    cGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTc                                                                                   >  6:276878/1‑99 (MQ=255)
                                                                                                    cGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTc                                                                                   <  5:276878/99‑1 (MQ=255)
                                                                                                      gCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCa                               <  8:75756/149‑1 (MQ=255)
                                                                                                          tCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGaa                            <  3:279614/148‑1 (MQ=255)
                                                                                                            gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGt                                       >  6:22074/1‑135 (MQ=255)
                                                                                                            gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGt                                       <  5:22074/135‑1 (MQ=255)
                                                                                                            gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                         <  6:213191/149‑1 (MQ=255)
                                                                                                            gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                         <  6:105529/149‑1 (MQ=255)
                                                                                                                 ccGAGACGTCGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                         >  2:227103/1‑144 (MQ=255)
                                                                                                                 ccGAGACGTCGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                         <  1:227103/144‑1 (MQ=255)
                                                                                                                    aGCCGTCGACAGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCga                 <  3:63579/149‑1 (MQ=255)
                                                                                                                     gCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCg                  >  4:224056/1‑147 (MQ=255)
                                                                                                                      acGTCGACACCGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCg                  >  5:264530/2‑146 (MQ=255)
                                                                                                                        gTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCgaag               >  6:294802/1‑147 (MQ=255)
                                                                                                                        gTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCgaag               <  5:294802/147‑1 (MQ=255)
                                                                                                                            aCGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTa         <  6:171779/149‑1 (MQ=255)
                                                                                                                             cGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGCGACAGCGAAGAAGCTAg        >  3:21014/1‑149 (MQ=255)
                                                                                                                             cGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAg        <  3:270300/149‑1 (MQ=255)
                                                                                                                                cgtCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGAc     >  4:31942/2‑149 (MQ=255)
                                                                                                                                    gACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGc  <  3:31942/148‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                               
CCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGC  >  NC_002947/4196166‑4196445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑